Promoter polynucleotide, signal polypeptide and use thereof
Provided are a promoter polynucleotide, a signal polypeptide and a polynucleotide encoding the signal polypeptide, and use thereof. A vector and a host cell each including the promoter polynucleotide and the polynucleotide encoding the signal polypeptide may efficiently express and/or extracellularly secrete a foreign protein.
1. A recombinant polynucleotide comprising a promoter having the nucleotide sequence of SEQ ID NO:1 and further comprising a first polynucleotide operably linked to the promoter, the first polynucleotide encoding a protein and the protein is a foreign protein with regard to Lactobacillus paracasei.
2. The recombinant polynucleotide of claim 1 , wherein the recombinant polynucleotide is a vector.
3. The recombinant polynucleotide of claim 2 , wherein the vector is a cloning vector or an expression vector.
4. The recombinant polynucleotide of claim 3 , wherein the vector is the expression vector.
5. The recombinant polynucleotide of claim 1 , further comprising a second polynucleotide encoding a signal polypeptide.
6. The recombinant polynucleotide of claim 5 , wherein the second polynucleotide encoding a signal polypeptide is operably linked between the promoter and the first polynucleotide.
7. The recombinant polynucleotide of claim 5 , wherein the second polynucleotide encoding a signal polypeptide is operably linked to the promoter and the first polynucleotide is fused in frame to the second polynucleotide encoding a signal polypeptide.
8. The recombinant polynucleotide of claim 5 , wherein the second polynucleotide encoding a signal polypeptide has the nucleotide sequence of SEQ ID NO:3.
9. A host cell comprising the recombinant polynucleotide of claim 1 .
10. The host cell of claim 9 , wherein the host cell is a lactic acid bacterium or belongs to the genus Escherichia.
11. The host cell of claim 10 , wherein the host cell is the lactic acid bacterium.
12. The host cell of claim 11 , wherein the lactic acid bacterium belongs to the genus Lactobacillus, Lactococcus, Bifidobacteria, Streptococcus, Leuconostoc, Weissella, Pediococcus , or Enterococcus.
13. The host cell of claim 9 , wherein the recombinant polynucleotide comprises a second polynucleotide encoding a signal polypeptide.
14. A method of producing a protein, the method comprising culturing the host cell of claim 9 in a medium; and recovering the protein from the cell or the medium.
15. The method of claim 14 , wherein the protein is recovered from the medium.
16. A method of producing a recombinant cell comprising introducing a vector to a host cell, wherein the host cell is Lactobacillus paracasei KCTC 13422BP, Lactobacillus plantarum KCTC 13421BP, or Lactobacillus brevis KCTC 13423BP, and wherein the vector comprises the recombinant polynucleotide of claim 1 .
17. A recombinant polynucleotide comprising a promoter having the nucleotide sequence of SEQ ID NO:1, wherein the recombinant polynucleotide is a vector, wherein the vector comprises E. coli ori, which is an origin of DNA replication of E. coli.