Campaign-ready series of recombinant adeno-associated virus (rAAV) complementing plasmids
In some aspects, the disclosure relates to compositions and methods for production of recombinant adeno-associated virus (rAAV) particles. The disclosure is based, in part, on isolated nucleic acids and systems that include only two vectors and are suitable for production of multiple different single gene viral vectors in a multiple small-scale campaign mode (e.g., 10 13 to 10 16 viral particles.)
1. A recombinant adeno-associated virus (rAAV) production system comprising:
(i) a first vector comprising the nucleic acid sequence set forth in any one of SEQ ID NOs: 1-9;
and,
(ii) a second vector comprising one or more nucleic acids encoding an expression cassette comprising a transgene flanked by adeno-associated virus inverted terminal repeat (ITR) sequences.
2. The rAAV production system of claim 1 , wherein the adeno-associated virus inverted terminal repeat (ITR) sequences are AAV2 ITRs.
3. The rAAV production system of claim 1 , wherein the transgene is a protein or an inhibitory nucleic acid, optionally wherein the inhibitory nucleic acid is dsRNA, siRNA, miRNA, or amiRNA.
4. The rAAV production system of claim 1 , wherein the expression cassette further comprises a promoter operably linked to the transgene, optionally wherein the promoter is a H1, U6, CB, CBA, CB6, Desmin, CMV, AAT, or MHK promoter.
5. The rAAV production system of claim 1 , further comprising a host cell.
6. An isolated nucleic acid comprising the sequence set forth in any one of SEQ ID NOs: 1-9.
7. A method for producing a recombinant adeno-associated virus (rAAV), the method comprising introducing the rAAV production system of claim 1 into a host cell that expresses an Ad-Ela helper function.
8. The method of claim 7 , wherein the first vector and second vector of the rAAV production system are introduced into the host cell in (i) a single transfection reaction or (ii) separate transfection reactions.
9. The method of claim 7 , further comprising the step of culturing the cells after introduction of the rAAV production system.
10. A method for producing a recombinant adeno-associated virus (rAAV), the method comprising:
(i) introducing the isolated nucleic acid of claim 6 into a host cell that expresses an Ad-Ela helper function; and
(ii) introducing a vector comprising one or more nucleic acids encoding an expression cassette comprising a transgene flanked by adeno-associated virus inverted terminal repeat (ITR) sequences.
11. The method of claim 10 , wherein the host cell is a mammalian cell.
12. The method of claim 10 , wherein the isolated nucleic acid and the vector are introduced into the host cell in (i) a single transfection reaction or (ii) separate transfection reactions.
13. The method of claim 10 , further comprising the step of culturing the cells after the introduction of the nucleic acid and the vector.
14. An apparatus for production of recombinant adeno-associated virus (rAAV) particles, the apparatus comprising:
(i) a container housing the rAAV production system of claim 1 ; and,
(ii) a population of host cells,
wherein the rAAV production system and the host cells are suspended in a cell culture medium.
15. The rAAV production system of claim 5 , wherein the host cell is a mammalian cell.
16. The method of claim 9 , wherein the culturing occurs in the presence of an antibiotic cognate to the antibiotic-resistance gene of the first vector.
17. The method of claim 10 , wherein the mammalian cell is a HEK293 cell, HEK293T cell, or a CHO cell.
18. The method of claim 13 , wherein the culturing occurs in the presence of an antibiotic cognate to the antibiotic-resistance gene of the first vector.