Nucleic acid-based botulinum neurotoxin for therapeutic use
The invention relates to a botulinum neurotoxin-encoding nucleic acid for therapeutic use. The invention further relates to the transfection of skeletal muscle cells and smooth muscle cells and the glands of the skin, and of other skin cells with botulinum neurotoxin (BoNT)-encoding nucleic acids (RNA or DNA) with or without the use of a secretory signal, for therapeutic and/or cosmetic purposes.
1. A nucleic acid for use as a drug and/or in therapeutic methods comprising a botulinum neurotoxin (BoNT)-encoding nucleic acid sequence, wherein the BoNT-encoding nucleic acid sequence is modified such that the linker region in the encoded protein between the light and heavy chains comprises a recognition motif for a protease and wherein the BoNT-encoding nucleic acid sequence, excluding the recognition motif for the protease, comprises a sequence according to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5 or a sequence having 80% sequence identity thereto.
2. A method of treating an organ or muscle in a subject, the method comprising directly applying the nucleic acid according to claim 1 to the organ and/or muscle to be treated.
3. The method according to claim 2 , wherein the subject is treated for spasticity, dystonia, saliva leakage, movement disorders, impaired muscle activity, osteosynthesis and/or hyperhydrosis.
4. A method of transfecting skeletal muscle cells, smooth muscle cells, smooth muscle cells of the glands or the skin and/or other skin cells, the method comprising transfecting the muscle cells, smooth muscle cells, smooth muscle cells of the glands or the skin and/or other skin cells with the nucleic acid according to claim 1 .
5. The nucleic acid according to claim 1 , wherein said nucleic acid is a DNA or RNA.
6. The nucleic acid according to claim 1 , wherein the BoNT-encoding nucleic acid sequence encodes BoNT/A, BoNT/B, BoNT/C, BoNT/D, BoNT/E, BoNT/F or BoNT/G.
7. The nucleic acid according to claim 1 , additionally comprising a secretory signal-encoding sequence.
8. The nucleic acid according to claim 1 additionally comprising a scaffold/matrix attachment region (S/MAR) element.
9. A method of cosmetically treating a subject, the method comprising administering a nucleic acid according to claim 1 to the subject, thereby cosmetically treating the subject.
10. The method of claim 9 , wherein said botulinum neurotoxin expressed in vivo is activated by a simultaneously or subsequently injected protease.
11. The method according to claim 9 , wherein the nucleic acid additionally comprising a secretory signal-encoding sequence.
12. The method according to claim 9 , wherein the nucleic acid additionally comprising a S/MAR element.
13. A pharmaceutical composition comprising a nucleic acid according to claim 1 and a pharmaceutically acceptable carrier and/or excipient.
14. The nucleic acid according to claim 1 , wherein the recognition motif for the respective protease is LVPRGS (SEQ ID NO: 7).