Autoimmune suppressor and application thereof
The present disclosure relates to a dual inhibiting antibody that targets human interleukin-4 and interleukin-13, a manufacturing method therefor and use thereof.
1. An antibody or a functional fragment thereof that specifically binds to IL-4Rα, wherein the antibody or functional fragment thereof comprises a heavy chain CDR1 selected from amino acid sequence SEQ ID NO: 172, a heavy chain CDR2 selected from amino acid sequence SEQ ID NO: 173, and a heavy chain CDR3 selected from amino acid sequence SEQ ID NO: 174; and a light chain CDR1 selected from amino acid sequence SEQ ID NO: 222, a light chain CDR2 selected from amino acid sequence SEQ ID NO: 223, and a light chain CDR3 selected from amino acid sequence SEQ ID NO: 224.
2. The antibody or functional fragment thereof according to claim 1 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 171, and a light chain variable region selected from amino acid sequence SEQ ID NO: 221.
3. The antibody or functional fragment thereof according to claim 1 , wherein the antibody or functional fragment thereof inhibits the interaction of hIL-4 with hIL-4Rα; preferably, the antibody or functional fragment thereof also inhibits the binding of hIL-4Rα to a complex hIL-13Rα/hIL-13; and optionally, the antibody or functional fragment thereof is humanized.
4. The antibody or functional fragment thereof according to claim 1 , wherein the antibody or a functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 176, and a light chain variable region selected from amino acid sequence SEQ ID NO: 221.
5. The antibody or functional fragment thereof according to claim 1 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 196, and a light chain variable region selected from amino acid sequence SEQ ID NO: 211.
6. The antibody or functional fragment thereof according to claim 1 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 196, and a light chain variable region selected from amino acid sequence SEQ ID NO: 216.
7. A method of treating an allergy, asthma, or chronic obstructive pulmonary disease, comprising administering to a mammalian patient in need thereof the antibody or functional fragment thereof according to claim 1 .
8. The method according to claim 7 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 171, and a light chain variable region selected from amino acid sequence SEQ ID NO: 221.
9. The method according to claim 7 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 176, and a light chain variable region selected from amino acid sequence SEQ ID NO: 221.
10. The method according to claim 7 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 196, and a light chain variable region selected from amino acid sequence SEQ ID NO: 211.
11. The method according to claim 7 , wherein the antibody or functional fragment thereof comprises a heavy chain variable region selected from amino acid sequence SEQ ID NO: 196, and a light chain variable region selected from amino acid sequence SEQ ID NO: 216.