IP Library › Granted Patent US 12,325,031
Granted Patent B2
US 12,325,031 · App. 16/972,858 · Granted Jun 10, 2025

Graphene-based dielectrophoresis sensor and method

Inventors: Steven John Koester (Edina, MN); Sang-Hyun Oh (Plymouth, MN)
Assignee: Regents of the University of Minnesota
B03C5/005G01N27/44756G01N33/487B03C2201/26
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Quick Facts
Patent No.
US 12,325,031
App. No.
16/972,858
Granted
Jun 10, 2025
Kind
B2
Abstract

A dielectrophoretic (DEP) sensor includes a graphene electrode adjacent a channel for confining a target particle in a liquid, a surface probe attached to a surface of the graphene electrode, the surface probe having a selective reaction with the target particle, and a voltage source electrically connected to the graphene electrode and configured to apply a voltage to the graphene electrode to cause DEP trapping of the target particle at the graphene electrode.

Claims (19)

1. A dielectrophoretic (DEP) sensor, comprising:

a graphene electrode adjacent a channel for confining a target particle in a liquid, the graphene electrode extending between a first electrical contact and a second electrical contact;

a surface probe attached to a surface of the graphene electrode, the surface probe having a selective reaction with the target particle;

a second electrode overlapping with the graphene electrode, the second electrode being connected to the second electrical contact;

a voltage source electrically connected to the second electrical contact and the second electrode and configured to vary a voltage between the graphene electrode and the second electrode to cause DEP trapping of the target particle at the graphene electrode; and

a source meter electrically connected to the first electrical contact and the second electrical contact and configured to apply a measurement signal to the graphene electrode, the measurement signal being sufficient to readout a response from the DEP sensor indicative of a presence of the target particle, measure a current through the graphene electrode in response to the measurement signal, and detect the presence of the target particle at the graphene electrode based on the current.

2. The DEP sensor of claim 1 , wherein the surface probe and the target particle are a complementary pair selected from the group consisting of molecules, proteins, aptamers, antibodies, lipids, vesicles, cell-derived particles, functionalized nanoparticles and whole cells.

3. The DEP sensor of claim 1 , wherein the graphene electrode comprises a plurality of strips.

4. The DEP sensor of claim 3 , wherein the plurality of strips are parallel to each other.

5. The DEP sensor of claim 1 , wherein the DEP sensor is a resistance-based sensor.

6. The DEP sensor of claim 1 , wherein the DEP sensor is a capacitance based sensor.

7. The DEP sensor of claim 1 , wherein the source meter is configured to apply a DC signal as the measurement signal.

8. The DEP sensor of claim 1 , wherein the source meter is configured to apply an AC signal as the measurement signal.

9. The DEP sensor of claim 1 , wherein the voltage source is configured to apply an AC excitation as the voltage and the source meter is configured so that an amplitude of the measurement signal is smaller than the AC excitation.

10. The DEP sensor of claim 3 , wherein each of the plurality of strips is electrically connected to a neighboring strip.

11. The DEP sensor of claim 10 , wherein the plurality of strips and electrical connections form a continuous, serpentine electrode that overlaps the second electrode.

12. The DEP sensor of claim 1 , wherein the second electrode comprises a plurality of parallel strips each connected at an end to a common strip, the common strip being connected to the second electrical contact.

13. The DEP sensor of claim 1 , wherein the graphene electrode and the second electrode are both planar electrodes.

14. The DEP sensor of claim 13 , wherein the graphene electrode and the second electrode are separated by an electrically insulating layer.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 15, 2021
From: KOESTER, STEVEN JOHN; OH, SANG-HYUN
To: REGENTS OF THE UNIVERSITY OF MINNESOTA
Reel/Frame 055926/0439 →
Continuity (2)
Provisional Application 62680777 · Jun 5, 2018
Related Publication 20210245172A1 · Aug 12, 2021
References Cited (101)
US 4647362A · Watanabe · 1987 [cited by applicant]
US 5569367A · Betts et al. · 1996 [cited by applicant]
US 7635420B1 · Li et al. · 2009 [cited by applicant]
US 10888875B2 · Oh et al. · 2021 [cited by applicant]
US 20030146100A1 · Huang · 2003 [cited by examiner]
US 20030157587A1 · Gomez et al. · 2003 [cited by applicant]
US 20060063183A1 · Segawa · 2006 [cited by examiner]
US 20060201811A1 · Hamers et al. · 2006 [cited by applicant]
US 20070246364A1 · Amlani et al. · 2007 [cited by applicant]
US 20110108422A1 · Heller et al. · 2011 [cited by applicant]
US 20110192726A1 · Chen et al. · 2011 [cited by applicant]
US 20120037919A1 · Xu et al. · 2012 [cited by applicant]
US 20120103813A1 · Sato · 2012 [cited by examiner]
US 20120134880A1 · Kurkina · 2012 [cited by examiner]
US 20130248368A1 · Kim et al. · 2013 [cited by applicant]
US 20140216935A1 · Vezenov · 2014 [cited by applicant]
US 20140291606A1 · Avouris et al. · 2014 [cited by applicant]
US 20150107999A1 · Weber et al. · 2015 [cited by applicant]
US 20150218630A1 · Sun et al. · 2015 [cited by applicant]
US 20170028408A1 · Menachery et al. · 2017 [cited by applicant]
US 20170292934A1 · Azpiroz · 2017 [cited by examiner]
US 20170368557A1 · Chi et al. · 2017 [cited by applicant]
US 20180361400A1 · Oh et al. · 2018 [cited by applicant]
US 20210220840A1 · Oh et al. · 2021 [cited by applicant]
CN 103531482 · 2014 [cited by applicant]
CN 107462621 · 2017 [cited by applicant]
EP 2434278 · 2012 [cited by applicant]
JP 2006084301 · 2006 [cited by applicant]
JP 2007006858 · 2007 [cited by applicant]
JP 2012065649 · 2012 [cited by applicant]
JP 2012247189 · 2012 [cited by applicant]
JP 2013224947 · 2013 [cited by applicant]
JP 2016180652 · 2016 [cited by applicant]
JP 2018009993 · 2018 [cited by applicant]
JP 2018021827 · 2018 [cited by applicant]
JP 2018036154 · 2018 [cited by applicant]
KR 20180033711 · 2018 [cited by applicant]
KR 101900049 · 2018 [cited by applicant]
KR 20200074785 · 2020 [cited by applicant]
KR 102134394 · 2020 [cited by applicant]
Xie et al., “Development of a 3D Graphene Electrode Dielectrophoretic Device,” Journal of Visualized Experiments, Jun. 2014, 88, e51696, 11 pages (Year: 2014). [cited by applicant]
Pumera et al., “Graphene in biosensing,” Mater. Today, Jul. 6, 2011, 14(7-8):308-315. [cited by applicant]
Suvarnaphaet et al., “Graphene-Based Materials for Biosensors: A Review,” Sensors, Sep. 21, 2017, 17(10):2161, 24 pages. [cited by applicant]
Ameri et al., “Utilization of graphene electrode in transparent microwell arrays for high throughput cell trapping and lysis,” Biosensors Bioelectronics., 61:625-630, 2014. [cited by applicant]
Barik et al., “Dielectrophoresis-Enhanced Plasmonic Sensing with Gold Nanohole Arrays,” Nano Lett., 14:2006-2012, 2014. [cited by applicant]
Barik et al., “Graphene-edge dielectrophoretic tweezers for trapping of biomolecules,” Nat. Communications, 8:1867, Nov. 2017, 9 pages. [cited by applicant]
Barik et al., “Ultralow-Power Electronic Trapping of Nanoparticles with Sub-10 nm Gold Nanogap Electrodes,” Nano Lett., 16:6317-6324, 2016. [cited by applicant]
Bonaccorso et al., “Graphene photonics and optoelectronics,” Nature Photonics., 4:611-622, Sep. 2010. [cited by applicant]
Chen et al., “Label-Free Detection of DNA Hybridization using Transistors Based on CVD Grown Graphene,” Biosensors Bioelectronics, 41:103-109, Mar. 2013. [cited by applicant]
Chou et al., “Electrodeless Dielectrophoresis of Single- and Double-Stranded DNA,” Biophys. Journal, 83(4):2170-2179, Oct. 2002. [cited by applicant]
Cinti et al., “Electrochemical Biosensors for Rapid Detection of Foodborne [cited by applicant]
Deen et al., “Graphene-Based Quantum Capacitance Wireless Vapor Sensors,” IEEE Sensors Journal, 14(5):1459-1466, Dec. 2013. [cited by applicant]
Dong et al., “Electrical Detection of DNA Hybridization with Single-Base Specificity Using Transistors Based on CVD-Grown Graphene Sheets,” Adv. Mater., 22(14):1649-1653, Apr. 2010. [cited by applicant]
Ebrish et al., “Operation of multi-finger graphene quantum capacitance varactors using planarized local bottom gate electrodes,” Appl. Phys. Letters, 100:143102, Apr. 2012, 4 pages. [cited by applicant]
Electroschematics.com [online], “Varactors,” Sep. 28, 2009, retrieved on Mar. 19, 2020, retrieved from URL<https://www.electroschematics.com/varactors/>, 2 pages. [cited by applicant]
EP Extended Search Report in EP Appln. No. 19816011.1, dated Jun. 22, 2021, 8 pages. [cited by applicant]
Fowler et al., Practical Chemical Sensors from Chemically Derived Graphene, ACS Nano., 3(2):301-306, 2009. [cited by applicant]
Freedman et al., “Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping,” Nat Commun., 7:10217, 2016, 9 pages. [cited by applicant]
Gascoyne et al., “Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments,” Proc. IEEE Inst. Electr. Electron Eng., 92(1):22-42, Jan. 2004. [cited by applicant]
Geiselmann et al., “Three-dimensional optical manipulation of a single electron spin,” Nat Nanotechnology., 8:175-179, Mar. 2013. [cited by applicant]
Giuliodori et al., “Development of a graphene oxide-based assay for the sequence-specific detection of double-stranded DNA molecules,” PLoS One, 12(8):e0183952, Aug. 2017, 17 pages. [cited by applicant]
Grigorenko et al., “Graphene plasmonics,” Nature Photonics., 6:749-758, Nov. 2012. [cited by applicant]
Guo et al., “Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of [cited by applicant]
Jose et al., “Individual Template-Stripped Conductive Gold Pyramids for Tip-Enhanced Dielectrophoresis,” ACS Photonics., 1:464-470, 2014. [cited by applicant]
Kakatkar et al., “Detection of DNA and Poly-l-lysine using CVD Graphene-Channel FET Biosensors,” Nanotechnology, 26(12):125502, Mar. 2015, 5 pages. [cited by applicant]
Kim et al., “Multitarget Dielectrophoresis Activated Cell Sorter,” Anal. Chem., 80(22):8656-8661, Nov. 2008. [cited by applicant]
Kurkina et al., “Label-free electrical biosensing based on electrochemically functionalized carbon nanostructures,” Dissertation for the degree of Doctor Rerum Naturalium, Institut fur Anorganische und Analytische Chemi… [cited by applicant]
Kuzyk., “Dielectrophoresis at the nanoscale,” Electrophoresis., 32:2307-2313, 2011. [cited by applicant]
Lee et al., “Optical separation of mechanical strain from charge doping in graphene,” Nat. Commun., 3:1024, Aug. 2012, 8 pages. [cited by applicant]
Li et al., “Graphene-templated supported lipid bilayer nanochannels,” Nano Lett., 16:5022-5026, 2016. [cited by applicant]
Li et al., “Large-Area Synthesis of High-Quality and Uniform Graphene Films on Copper Foils,” Science, 324(5932):1312-1314, Jun. 2009. [cited by applicant]
Liu et al., “Biological and chemical sensors based on graphene materials,” Chem. Soc. Rev., 41(6):2283-2307, Mar. 2012. [cited by applicant]
Loan et al., “Hall Effect Biosensors with Ultraclean Graphene Film for Improved Sensitivity of Label-Free DNA Detection,” Biosensors Bioelectronics, 99:85-91, Jan. 2018. [cited by applicant]
Low and Avouris., “Graphene Plasmonics for Terahertz to Mid-Infrared Applications,” ACS Nano., 8(2):1086-1101, 2014. [cited by applicant]
Low et al., “Polaritons in layered two-dimensional materials,” Nature Mater., 16:182-194, Feb. 2017. [cited by applicant]
Lukacs et al., “Size-dependent DNA Mobility in Cytoplasm and Nucleus,” J Biol Chem., 275(3):1625-1629, Jan. 21, 2000. [cited by applicant]
Ma et al., “Acetone Sensing Using Graphene Quantum Capacitance Varactors,” 2016 IEEE Sensors Conference, Orlando, Florida, USA, Oct. 30-Nov. 3, 2016, 3 pages. [cited by applicant]
Neto et al., “The electronic properties of graphene,” Rev Mod Phys., 81:109-162, Jan.-Mar. 2009. [cited by applicant]
Novoselov et al., “Electric Field Effect in Atomically Thin Carbon Films,” Science, 306(5696):666-669, Oct. 2004. [cited by applicant]
Park et al., “Development of multiplex PCR assay for simultaneous detection of [cited by applicant]
PCT International Preliminary Report on Patentability in International Appln. No. PCT/US2019/035558, dated Dec. 8, 2020, 6 pages. [cited by applicant]
PCT International Search Report and Written Opinion in International Appln. No. PCT/US2019/035558, dated Sep. 10, 2019, 8 pages. [cited by applicant]
Pelton., “Modified spontaneous emission in nanophotonic structures,” Nature Photonics., 9:427-435, Jul. 2015. [cited by applicant]
Ping et al., “Scalable Production of High-Sensitivity, Label-Free DNA Biosensors Based on Back-Gated Graphene Field Effect Transistors,” ACS Nano, 10(9):8700-8704, Aug. 2016. [cited by applicant]
Regtmeier et al., “Dielectrophoretic Trapping and Polarizability of DNA: The Role of Spatial Conformation,” Anal Chem., 82(17):7141-7149, Sep. 1, 2010. [cited by applicant]
Rodrigo et al., “Mid-infrared plasmonic biosensing with graphene,” Science., 349(6244):165-168, Jul. 10, 2015. [cited by applicant]
Sanghavi et al., “Electrokinetic Preconcentration and Detection of Neuropeptides at Patterned Graphene-Modified Electrodes in a Nanochannel,” Anal. Chemistry, 86(9):4120-4125, May 2014. [cited by applicant]
Schneider et al., “Tailoring the hydrophobicity of graphene for its use as nanopores for DNA translocation,” Nat Commun., 4:2619, 2013, 7 pages. [cited by applicant]
Sheehan, and L. J. Whitman, “Detection Limits for Nanoscale Biosensors,” Nano Lett., 5(4):803-807, Apr. 2005. [cited by applicant]
Squires et al., “Making it stick: convection, reaction and diffusion in surface-based biosensors,” Nature Biotechnology., 26(4):417-426, Apr. 2008. [cited by applicant]
UMN.edu [online], “Researchers develop graphene nano ‘tweezers’ that can grab individual biomolecules,” Dec. 4, 2017, retrieved on Jul. 7, 2021, retrieved from URL<https://cse.umn.edu/college/news/researchers-develop-gr… [cited by applicant]
WHO.int [online], “Food safety,” available on or before Nov. 21, 2014 via Internet Archive: Wayback Machine URL<https://web.archive.org/web/20141121212113/http://www.who.int/mediacentre/factsheets/fs399/en/>, retrieved … [cited by applicant]
Xu et al., “Electrophoretic and Field-Effect Graphene for All-Electrical DNA Array Technology,” Nat. Commun., 5:4866, Sep. 2014, 9 pages. [cited by applicant]
Xu et al., “Nucleic Acid Biosensor Synthesis of an All-in-One Universal Blocking Linker Recombinase Polymerase Amplification with a Peptide Nucleic Acid-Based Lateral Flow Device for Ultrasensitive Detection of Food Pat… [cited by applicant]
Yan et al., “Damping pathways of mid-infrared plasmons in graphene nanostructures,” Nature Photonics., 7:394-399, May 2013. [cited by applicant]
Zhang et al., “Capacitive sensing of glucose in electrolytes using graphene quantum capacitance varactors,” ACS Appl. Mater. Interfaces, 9(44):38863-38869, Oct. 2017. [cited by applicant]
Zhang et al., “Glucose sensing with graphene varactors,” 2016 IEEE Sensors Conference, Orlando, Florida, USA, Oct. 30-Nov. 3, 2016, 3 pages. [cited by applicant]
Zheng et al., “Manipulating Nanoparticles in Solution with Electrically Contacted Nanotubes Using Dielectrophoresis,” Langmuir, 20(20):8612-8619, Sep. 28, 2004. [cited by applicant]
U.S. Appl. No. 16/010,980, filed Jun. 18, 2018, Sang-Hyun Oh. [cited by applicant]
U.S. Appl. No. 17/121,285, filed Dec. 14, 2020, Sang-Hyun Oh. [cited by applicant]
Suehiro et al., “Selective detection of bacteria using dielectrophoretic impedance measurement method combined with antigen-antibody reaction,” Conference Record of the 2001 IEEE Industry Applications Conference, 36th I… [cited by applicant]