Transformant, and method using said transformant to detect presence or absence of reduced phosphorous compound
It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.
1. A cyanobacterial transformant comprising a disrupted gene encoding a phosphate transporter protein, a disrupted gene encoding a phosphate ester transporter protein, and an inserted gene encoding a hypophosphite transporter protein, wherein:
the cyanobacterial transformant is incapable of utilizing phosphate for proliferation but is capable of utilizing phosphite for proliferation;
the hypophosphite transporter protein includes a hypophosphite binding protein as a constituent element; and
a gene encoding a PtxB signal peptide derived from Anabaena sp. PCC7120 is substituted for a gene encoding a signal peptide of the hypophosphite binding protein.
2. The cyanobacterial transformant as set forth in claim 1 , further comprising an inserted gene encoding a phosphite dehydrogenase protein.
3. The cyanobacterial transformant as set forth in claim 1 , further comprising a disrupted gene encoding an alkaline phosphatase protein.
4. A method comprising;
culturing the cyanobacterial transformant of claim 1 in a culture medium; and
after culturing, detecting the cyanobacterial transformant proliferation, which is indicative of the presence of reduced phosphorous compound in the culture medium.