Methods of inducing insulin production
The present invention provides a method of inducing insulin production in a cell by up-regulating a target gene involved in insulin production in said cell using an saRNA (short activating ribonucleic acid) which specifically targets a target antisense RNA transcript present in said cell.
1. An saRNA which upregulates a target gene involved in insulin production in a cell, wherein the target gene is Neurogenin 3 (Ngn3), and wherein said saRNA comprises a strand of up to 30 nucleotides in length comprising a sequence selected from SEQ ID No. 11-14.
2. The saRNA of claim 1 , wherein said saRNA is a single-stranded RNA.
3. The saRNA of claim 1 , wherein said saRNA is a double-stranded RNA.
4. The saRNA of claim 3 , wherein each strand of the double-stranded RNA has 19 to 25 base pairs in length with 1-5 unpaired bases on each 3′ end of the strand forming a 3′ overhang.
5. The saRNA of claim 4 , wherein said 3′ overhand is formed of 2 uracils.
6. The saRNA of claim 1 , comprising at least one modification.
7. The saRNA of claim 1 , wherein the cell is induced to produce insulin in a glucose-responsive manner.
8. The saRNA of claim 1 , wherein the cell is induced to secrete insulin.
9. The saRNA of claim 1 , wherein the cell is an adult or embryonic stem cell, a pluripotent cell, a multipotent cell, or a somatic cell.
10. The saRNA of claim 1 , wherein the cell is a liver cell or a pancreatic cell.
11. A pharmaceutical composition comprising one or more saRNAs according to claim 1 .