Method of producing natural killer cells and composition for treating cancer

1. A method of producing natural killer cells, comprising:

co-culturing PBMCs with a combination of feeder cells in the presence of IL-2 and IL-21.

2. The method of claim 1 , further comprising isolating at least one of CD56+ cells and/or CD3−/CD56+ cells and/or CD3− from the PBMCs, which is conducted by using at least one of CD56 microbeads and CD3 microbeads.

3. The method of claim 1 , wherein the combination of feeder cells comprises irradiated Jurkat cells and irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells.

4. The method of claim 3 , wherein the ratio of the irradiated Jurkat cells and the irradiated EBV-LCL cells is about 1:0.1-5 or 0.1-5:1.

5. The method of claim 3 , wherein each of the irradiated Jurkat cells and the irradiated EBV-LCL cells are obtained by irradiation of 50-500 Gy.

6. The method of claim 3 , wherein the co-culturing comprises co-culturing for 1-50 days.

7. The method of claim 3 , further comprising:

co-culturing the PBMCs cells with a combination of feeder cells, in the presence of IL-2 for a first period; and

subsequently co-culturing the PBMCs with the combination of feeder cells, in the presence of IL-21 for a second period.

8. The method of claim 7 , wherein the IL-21 is added once or more during the first six days of every fourteen-day cycle during the second period.

9. The method of claim 3 , wherein the IL-21 is added once or more during Day 0-6 of the second period.

10. The method of claim 9 , wherein the IL-21 and the combination of feeder cells are added once or more during Day 0-6 of the second period.

11. A composition made by the method of claim 1 .

12. The method of claim 1 , wherein IL-2 is present at a concentration of 50-1000 IU/ml and IL-21 is present at a concentration of 10-100 ng/ml.

13. A composition for treating cancer in a patient in need thereof, comprising:

an effective amount of CD56+ natural killer cells derived from peripheral blood, wherein the effective amount is in a range of about 1×10 6 to 5×10 8 cells per kg of the patient's body weight, and wherein the CD56+ natural killer cells are at least about 90% pure;

IL-2 having a concentration of 50-50,000 IU/mL; and

a pharmaceutically acceptable carrier.

14. A method of making a therapeutic composition, the method comprising:

co-culturing PBMCs with a combination of feeder cells in the presence of one or more cytokines to produce an expanded NK cell population; and

storing the expanded NK cell population in a storage solution that comprises IL-2, wherein the IL-2 is present in an amount sufficient to inhibit apoptosis of the expanded NK cell population, wherein the expanded NK cell population is stored for at least 48 hours, and wherein the expanded NK cell population is at least about 90% pure.

15. A method of treating a human subject in need of cell immunotherapy, comprising:

storing at least a therapeutically effective amount of an expanded NK cell population in a storage solution that comprises IL-2, wherein the IL-2 is present in an amount sufficient to inhibit apoptosis of the expanded NK cell population, wherein the expanded NK cell population comprises CD56+ cells and/or CD3−/CD56+ cells, and wherein the expanded NK cell population is stored for at least 48 hours; and

administering the therapeutically effective amount of the expanded NK cell population to the human subject.

16. A method of treating a cancer, comprising:

administering a therapeutic composition to a patient, the therapeutic composition comprising:

an effective amount of at least one of CD56+ and/or CD3−/CD56+ natural killer cells derived from peripheral blood mononuclear cells (PBMCs), wherein the effective amount is in a range of about 1×10 6 to 5×10 8 cells per kg of the patient's body weight, and wherein the natural killer cells are at least about 90% pure;

IL-2 having a concentration of 50-50,000 IU/mL; and

a pharmaceutically acceptable carrier.

17. A method of treating a cancer, comprising:

administering a therapeutic composition to a patient who has cancer, the therapeutic composition comprising having been prepared by co-culturing PBMCs with a combination of feeder cells in the presence of IL-2 at a concentration of 50-1000 IU/ml and IL-21 at a concentration of 10-100 ng/ml.

18. A method of expanding human natural killer cells in culture, comprising:

isolating at least one of human CD56+ cells and/or human CD3−/CD56+ cells from the human PBMCs; and

co-culturing the at least one of human CD56+ cells and/or human CD3−/CD56+ cells with a combination of human feeder cells in the presence of at least two human cytokines;

wherein the combination of feeder cells comprises irradiated Jurkat cells and irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells,

wherein the at least two cytokines comprise IL-2 and IL-21.