Test device
The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed.
1. A method of performing an identification testing or antimicrobial susceptibility of bacteria or fungi, including the steps of:
preparing a suspension of either the bacteria or the fungi; culturing the bacteria or the fungi by adding the suspension into a microplate having a plurality of wells that each contain a culture fluid and a different one of a plurality of antimicrobial agents or a different one of a plurality of antifungal agents, and mixing the suspension and culture fluid in the microplate;
obtaining at multiple times during an observation time, via an input device, a plurality of images by performing microscopic observation of a state of culturing the bacteria or the fungi;
performing, via a processor, on the plurality of images obtained by the microscopic observation at least one image processing of binarization processing, grey scale image processing, processing of calculating areas of the bacteria or the fungi, processing of calculating a number of the bacteria or the fungi, processing of calculating a circularity of the bacteria or the fungi, processing of calculating an aspect ratio of the bacteria or the fungi, or processing of calculating a perimeter length of the bacteria or the fungi;
generating, via the processor, based on a result of the image processing, influence determination information showing influence given by a drug to the bacteria or the fungi, with respect to plural types of drugs and plural types of concentrations; and
displaying, via a display, the influence determination information in a time series based on the observation time;
wherein each of the different one of the plurality of antimicrobial agents or each of the different one of a plurality of antifungal agents are respectively contained in the culture fluid of each of the plurality of wells; and
wherein the step of generating the influence determination information includes:
determining whether the bacteria or the fungi proliferate or proliferation thereof is interrupted under a predetermined culture condition by comparing the plurality of images obtained by the microscopic observation, specifying a minimum inhibitory concentration by determining whether the bacteria or the fungi proliferate or the proliferation thereof is interrupted under the plurality of concentration conditions, with respect to a target drug, performing antimicrobial susceptibility determination of the bacteria or the fungi by comparing the minimum inhibitory concentration with a break point after specifying the minimum inhibitory concentration in the target drug, and wherein the step of displaying includes: displaying a result of the antimicrobial susceptibility determination on the display.
2. The method according to claim 1 ,
wherein the step of generating the influence determination information includes: determining, at predetermined time intervals, whether or not the bacteria or the fungi proliferate or the proliferation thereof is interrupted under each of the plurality of concentration conditions of the target drug.
3. The method according to claim 1 ,
wherein the step of generating the influence determination information includes: generating a graph which plots a time series change of areas of the bacteria or the fungi by acquiring, at predetermined time intervals, the areas of the bacteria or the fungi based on a result of the microscopic observation on whether or not the bacteria or the fungi proliferate under each of the plurality of concentration conditions of the target drug, and
wherein the step of displaying includes: displaying the generated graph the display.
4. The method according to claim 1 ,
wherein the step of displaying includes: aligning and displaying the images obtained by the microscopic observation under a target culture condition on the display in a time series.
5. The method according to claim 1 ,
wherein the step of displaying includes: aligning and displaying the images obtained by the microscopic observation in accordance with the plurality of concentration conditions of the target drug.
6. The method according to claim 1 , wherein the step of displaying includes: displaying, at predetermined time intervals, the result of the antimicrobial susceptibility determination on the display, and displaying the result of the antimicrobial susceptibility determination regarding the plurality of bacteria or fungi in parallel.
7. The method according to claim 1 ,
wherein the step of displaying includes: displaying, at predetermined time intervals, the result of the antimicrobial susceptibility determination of different patients on the display to be capable of distinguishing strains of the bacteria or the fungi by comparison.
8. The method according to claim 1 ,
wherein the step of generating the influence determination information includes: determining whether the bacteria or the fungi proliferate or proliferation thereof is interrupted under a bacteria identification culture condition or a predetermined culture condition and determining whether the bacteria or the fungi proliferate or proliferation thereof is interrupted under plural concentration conditions with respect to a target drug, by comparing the images obtained by the microscopic observation with images accumulated in a database which stores images of the bacteria of fungi in a case where culture is performed with each type of drugs and each type of concentrations for each species of the bacteria or the fungi.
9. The method according to claim 1 , wherein the suspension is prepared using a single colony of either the bacteria or the fungi.