SPORE-BASED PROBIOTIC SUPPLEMENTATION IN CHICKENS AND EFFECT ON SALMONELLA LOADS
This disclosure relates to a method of evaluating effects of probiotic supplementation on performance and Salmonella load in broilers challenged with Salmonella enterica serovar Enteritidis, said probiotic containing Bacillus subtilis, Bacillus licheniformis, and a cellulose carrier.
1 . A method for treating a chicken for a Salmonella infection, comprising the steps of
administering to the chicken in need of such treatment an effective amount of a spore-based probiotic composition comprising strains Bacillus subtilis (HU58) and Bacillus licheniformis SL-307, each strain comprising Bacillus spores, wherein the Salmonella infection is reduced in severity.
2 . The method of claim 1 , wherein the probiotic composition comprises a chicken feed including a cellulose carrier.
3 . The method of claim 1 , wherein the chicken feed comprises Bacillus subtilis (HU58) at a dose of 10 mg/kg feed and Bacillus licheniformis SL-307 at a dose of 100 mg/kg feed.
4 . The method of claim 1 , wherein a caecal bacterial load of the Salmonella infection is reduced by about 25% copy numbers of S. enteritidis expressed in log 10 CFU/g of caecal contents after about 5 days of treatment.
5 . The method of claim 1 , wherein a caecal bacterial load of the Salmonella infection is reduced by about 50% copy numbers of S. enteritidis expressed in log 10 CFU/g of caecal contents after about 10 days of treatment.
6 . The method of claim 1 , wherein the Salmonella infection is derived from Salmonella enterica serovar Enteritidis.
7 . A method for treating or preventing a Salmonella infection in a chicken, comprising the steps of
administering to the chicken in need of such treatment an effective amount of a spore-based probiotic composition comprising strains Bacillus subtilis (HU58) and Bacillus licheniformis SL-307, each strain comprising Bacillus spores.
8 . The method of claim 7 , wherein the probiotic composition comprises a chicken feed including a cellulose carrier.
9 . The method of claim 7 , wherein the chicken feed comprises Bacillus subtilis (HU58) at a dose of 10 mg/kg feed and Bacillus licheniformis SL-307 at a dose of 100 mg/kg feed.
10 . The method of claim 7 , wherein a caecal bacterial load of the Salmonella infection is reduced by about 25% copy numbers of S. enteritidis expressed in log 10 CFU/g of caecal contents after about 5 days of treatment.
11 . The method of claim 7 , wherein a caecal bacterial load of the Salmonella infection is reduced by about 50% copy numbers of S. enteritidis expressed in log 10 CFU/g of caecal contents after about 10 days of treatment.
12 . The method of claim 7 , wherein the Salmonella infection is derived from Salmonella enterica serovar Enteritidis.