Granted Patent
B2
US 12,247,974 · App. 17/027,029 · Granted Mar 11, 2025
Device and method for chemical analysis
Inventors:
Mohammad E. Taslim (Needham, MA); Mohammed Fotouhi (Weston, MA); Mehdi Abedi (Brighton, MA); Reza Mollaaghababa (Natick, MA); Bahram Fotouhi (Cupertino, CA); Kashayar Javaherian (Lexington, MA); Edward Alvin Greenfield (Stoughton, MA); Namal Nawana (Weston, MA)
Assignee:
Graphene-Dx, Inc.
G01N33/5302B01L3/5027G01N1/286G01N1/4044G01N1/4077G01N27/045G01N33/02G01N33/025G01N33/5308G01N33/54366G01N33/5438G01N33/544G01N33/551G01N33/552G01N33/68B01L2200/04B01L2300/023B01L2300/0663G01N2001/2866G01N2001/4088G01N2333/415
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Abstract
A processing and detection system for detecting presence of at least one gluten protein in a food sample comprises a food processor including: a reservoir containing a process liquid for processing the food sample; a body that comprises a chamber configured to receive the food sample; and a pressing surface configured to press on the reservoir to cause the process liquid to exit the reservoir and mix with the food sample, thereby generating a processed food liquid; and an exit port configured to conduct the processed food liquid out of the food processor; and a cartridge including: at least one sensor configured to receive the processed food liquid and to generate an electrical signal in response to interaction with the at least one gluten protein in the processed food liquid, and an analyzer in electrical communication with the at least one sensor for detecting the electrical signal and determining the presence of the at least one gluten protein in the food sample based on the detected electrical signal.
Claims (16)
1. A system, comprising:
a cartridge, comprising:
a first sensor comprising a first graphene layer disposed on an underlying substrate, wherein said first graphene layer is functionalized with a first antibody exhibiting specific binding to a first analyte, and
a second sensor comprising a second graphene layer disposed on said underlying substrate, wherein said second graphene layer is functionalized with a second antibody exhibiting specific binding to a second analyte different than said first analyte,
at least one measurement circuitry in communication with said first and said second sensor for generating first and second signals in response to a change in at least one electrical property of said first and said second sensor, respectively, in response to interaction of said first and said second graphene layer with a first and second portion of a liquid sample, respectively, and
an analyzer comprising a processor and a memory storing instructions, wherein the processor is configured to receive said first and said second signals and execute the instructions to employ the second signal as a calibration signal against which the first signal is evaluated.
2. The system of claim 1 , wherein the cartridge further comprises a sample delivery component having an inlet for receiving the liquid sample and a plurality of channels for distributing the first portion and the second portion of said received liquid sample to said first and second sensor, respectively.
3. The system of claim 1 , wherein each of said first and second sensor comprises a plurality of conductive pads electrically coupled to the respective functionalized graphene layer to allow said at least one measurement circuitry to measure said at least one electrical property of that functionalized graphene layer.
4. The system of claim 1 , wherein said underlying substrate comprises a semiconductor.
5. The system of claim 4 , wherein said semiconductor comprises silicon.
6. The system of claim 5 , further comprising a silicon dioxide layer disposed between said silicon and said first and second graphene layers.
7. The system of claim 1 , further comprising at least one linker for coupling said first and second antibodies to said first and second sensor, respectively.
8. The system of claim 7 , wherein said at least one linker comprises a first linker that is coupled to the first graphene layer via pi-pi interaction at one end thereof and is covalently attached to said first antibody at a second end thereof and a second linker that is coupled to the second graphene layer via pi-pi interaction at one end thereof and is covalently attached to said second antibody at a second end thereof.
9. The cartridge of claim 1 , wherein said second antibody is an isotype of said first antibody.
10. The system of claim 1 , wherein at least one of said first and second sensor is functionalized with an antibody exhibiting specific binding to a pathogen.
11. The system of claim 1 , wherein said underlying substrate is formed of a material selected from the group comprising: silicon, polymeric materials, polyurethane, polyethylene terephthalate, and glass.
Assignments (8)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 10, 2025
From: NAWANA, NAMAL
To: GRAPHENE-DX, INC.
Reel/Frame 070163/0608 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 5, 2025
From: NAWANA, NAMAL
To: GRAPHENE-DX, LLC
Reel/Frame 070114/0973 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 8, 2024
From: TASLIM, MOHAMMAD E.; FOTOUHI, MOHAMMED; ABEDI, MEHDI; MOLLAAGHABABA, REZA; FOTOUHI, BAHRAM; JAVAHERIAN, KASHAYAR; GREENFIELD, EDWARD
To: RITE TASTE, LLC
Reel/Frame 069219/0339 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 17, 2021
From: TASLIM, MOHAMMAD E.; FOTOUHI, MOHAMMED; ABEDI, MEHDI; MOLLAAGHABABA, REZA; FOTOUHI, BAHRAM; JAVAHERIAN, KASHAYAR; GREENFIELD, EDWARD ALVIN
To: RITE TASTE, LLC
Reel/Frame 055621/0360 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 17, 2021
From: NAWANA, NAMAL
To: GRAPHENE-DX, LLC
Reel/Frame 055621/0392 →
Continuity (7)
Provisional Application
62676079
· May 24, 2018
Provisional Application
62206471
· Aug 18, 2015
Provisional Application
62059731
· Oct 3, 2014
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