In vitro and in vivo intracellular delivery of siRNA via self-assembled nanopieces
The compositions and methods of the invention provide compositions and methods for preferential targeting of tissues to delivery therapeutic or diagnostic agents. For example, such compounds are useful in the treatment of joint disorders those affecting articulating joints, e.g., injury-induced osteoarthritis as well as autoimmune diseases affecting joint tissue such as rheumatoid arthritis.
1. A method of treating rheumatoid arthritis comprising the systemic administration of an effective amount of a rosette nanopiece and a nucleic acid, wherein said nanopiece comprises a compound of Formula I or Formula II,
or a salt thereof,
wherein,
X is CH or N;
R 2 is hydrogen or a linker group;
Y is absent when R 2 is hydrogen or is an amino acid or polypeptide;
R 1 is hydrogen or aliphatic,
wherein the amino acid side chain is selected from:
wherein, the compounds of Formula (I), Formula (II), or a salt thereof, or a combination of Formula (I) and Formula (II) self-assemble to form a nanotube, and
wherein the nucleic acid comprises an siRNA selected from the group consisting of TNF-α siRNA, a sequence comprising AAG CCT GTA GCC CAC GTC GTA (SEQ ID NO: 229), a sequence comprising GGC ACC ACT AGT TGG TTG TCT TTG (SEQ ID NO: 230), an anti-miR-181a comprising SEQ ID NO: 228, and an anti-miR-181a comprising SEQ ID NO: 229.
2. The method of claim 1 , wherein the nanopiece comprises a compound selected from
or a salt thereof.