Method for adenovirus purification

1. A method for adenovirus purification comprising the following steps: a) capturing adenovirus from an adenovirus-containing cell culture harvest on an anion exchanger resin, wherein the anion exchange resin comprises an agarose base matrix modified with a quaternary ammonium strong ion exchange ligand; b) eluting said adenovirus with a shallow conductivity gradient with an increasing salt concentration (mM) of 15-25% over the gradient; c) adding said eluted adenovirus to a shell bead resin comprising a porous, inert shell and a porous core, wherein the core is provided with hydrophobic interaction ligands; and d) eluting said adenovirus from said shell bead resin in the flow through, wherein the adenovirus eluted in step d) comprises less than 1 ng/ml host cell protein (HCP).

2. The method according to claim 1 , wherein the salt is selected from NaCl, KCl and LiCl, or any combinations thereof.

3. The method according to claim 1 , wherein the salt is NaCl and the gradient is increasing 18-20% and the salt concentration is between 0-700 mM.

4. The method according to claim 1 , wherein the anion exchange resin is packed in a column and the shell bead resin is packed in another column, and wherein the adenovirus eluted from the anion exchanger resin is added to the shell bead resin in a volume corresponding to 15-30 column volumes (CV) of the column comprising shell bead resin.

5. The method according to claim 4 , wherein the adenovirus eluted from the anion exchanger resin is added to the shell bead resin in a volume corresponding to 25-30 column volumes (CV) of the column comprising shell bead resin.

6. The method according to claim 1 , wherein the porosity of the core and shell is the same of the shell bead resin.

7. The method according to claim 1 , wherein the porosity of the core and shell is different of the shell bead resin.