METHODS FOR MEASURING AND STABILIZING STAT3 INHIBITORS
Methods for stabilizing Stat3 inhibitors in a blood sample are provided. Methods for assessing the level of such Stat3 inhibitors are also provided.
1 . A method for the treatment of a blood sample in vitro, the method comprising mixing the blood sample in vitro with a composition comprising a fluoride salt, wherein said blood sample is preserved for further analysis.
2 . The method of claim 1 , wherein the fluoride salt comprises LiF, NaF, KF, CsF, or NH4F.
3 . The method of claim 1 , wherein the fluoride salt comprises sodium fluoride.
4 . The method of claim 1 , wherein a compound of Formula III in the blood sample is stabilized.
5 . The method of claim 4 , wherein the compound of Formula III is stabilized at room temperature for at least two hours after blood collection.
6 . The method of claim 1 , wherein the composition is provided in a blood collection device.
7 . The method of claim 6 , wherein the blood sample is placed in the blood collection device and the mixing is performed in the blood collection device.
8 . The method of claim 7 , wherein the blood sample is stored in the blood collection device for a predetermined period of time during which the level of the compound of Formula III is substantially constant.
9 . The method of claim 8 , wherein the predetermined period of time is at least two hours
10 . The method of claim 1 , wherein the blood sample and the composition are mixed at a 1:1 ratio.
11 . The method of claim 1 , wherein the blood sample is from a patient who has been administered either a compound of Formula III.
12 . The method of claim 1 , wherein the method is further defined as a method for stabilizing a compound of Formula III in the blood sample.
13 . The method of claim 1 , wherein the blood sample is a whole blood sample, a plasma sample, or a serum sample.
14 . The method of claim 1 , wherein the composition further comprises sodium sulfite.
15 . The method of claim 1 , wherein the composition further comprises ascorbic acid.
16 . The method of claim 1 , wherein the composition further comprises an anticoagulant.
17 . A method for determining the amount of a compound of Formula III in a blood sample, the method comprising:
(a) obtaining a blood sample from a patient who has been administered a compound of Formula III;
(b) performing the method of claim 1 on the blood sample;
(c) storing the treated blood for a predetermine period of time during which the level of the compound of Formula III is substantially constant; and
(d) determining the amount of the compound of Formula III in the blood sample.
18 . The method of claim 17 , wherein steps (a) and (b) occur simultaneously.
19 . The method of claim 17 , wherein the predetermined period of time is less than 30 minutes.
20 . The method of claim 17 , wherein the predetermined period of time is at least two hours.
21 . The method of claim 17 , wherein the predetermined period of time is at most four hours.
22 . A blood collection device comprising a composition comprising a fluoride salt, sodium sulfite, and ascorbic acid.
23 . The blood collection device of claim 22 , wherein the fluoride salt comprises LiF, NaF, KF, CsF, or NH4F.
24 . The blood collection device of claim 22 , wherein the fluoride salt is sodium fluoride.
25 . The blood collection device of claim 22 , further comprising an anticoagulant.
26 . The blood collection device of claim 22 , further comprising a blood sample that is preserved for further analysis.
27 . The blood collection device of claim 26 , wherein a compound of Formula III in the blood sample are stabilized.
28 . The blood collection device of claim 22 , where the device is a blood collection tube, an evacuated blood collection tube, a vacutainer, or an aspiration system.
29 . A kit comprising:
(a) the blood collection device of claim 22 ; and
(b) test substances for determining a level of a compound of Formula III.
30 . A method for stabilizing a compound of Formula III in a blood sample in vitro, the method comprising adjusting the pH of the blood sample to about 5, wherein said blood sample is preserved for further analysis.
31 . The method of claim 30 , wherein the compound of Formula III and/or the compound of Formula IIIx is stabilized at room temperature for at least two hours after blood collection.
32 . The method of claim 30 , wherein the blood sample is from a patient who has been administered either a compound of Formula III.
33 . The method of claim 30 , wherein the blood sample is a whole blood sample, a plasma sample, or a serum sample.
34 . A method for determining the amount of a compound of Formula III in a blood sample, the method comprising:
(a) obtaining a blood sample from a patient who has been administered a compound of Formula III;
(b) performing the method of claim 30 on the blood sample;
(c) storing the treated blood for a predetermine period of time during which the level of the compound of Formula III is substantially constant; and
(d) determining the amount of the compound of Formula III in the blood sample.
35 . The method of claim 34 , wherein steps (a) and (b) occur simultaneously.
36 . The method of claim 34 , wherein the predetermined period of time is less than 30 minutes.
37 . The method of claim 34 , wherein the predetermined period of time is at least two hours.
38 . The method of claim 34 , wherein the predetermined period of time is at most four hours.