Expression of unfolded protein response proteins improves plant biomass and growth
Described herein are expression cassettes, plant cells, plant seeds, plants, and methods useful for improving the glucan content and growth of plants.
1. A plant cell, plant seed, or plant comprising an expression system comprising (a) a first expression cassette comprising a first promoter operably linked to a nucleic acid segment encoding an IRE1 polypeptide comprising an amino acid sequence with at least 95% sequence identity to any of SEQ ID NO: 9 and 11, 12, 13, 14, or 15; and (b) a second expression cassette comprising a second promoter operably linked to a nucleic acid segment encoding a CSLF6 polypeptide comprising an amino acid sequence with at least 95% sequence identity to any of SEQ ID NO: 1, 5, 6, 7, or 8, wherein the plant cell, plant seed, or plant are selected from the group consisting of Brachypodium distachyon , wheat, barely, corn, rice, or sorghum.
2. The plant of claim 1 , wherein a population of the plants having the expression system has an average dry stem mass that is at least 5% greater than an average dry stem mass of a corresponding wild type population of plants of the same age, where the wild type population of plants does not have the expression system.
3. The plant of claim 1 , wherein a population of the plants having the expression system has an average glucan content that is at least 5% greater than a glucan content of a corresponding wild type population of plants of the same age, where the wild type population of plants does not have the expression system.
4. The plant cell, plant seed, or plant of claim 1 , wherein the first promoter or the second promoter is a strong or inducible promoter.
5. The plant cell, plant seed, or plant of claim 1 , wherein the first promoter or the second promoter is a tissue-specific promoter.
6. The plant cell, plant seed, or plant of claim 1 , wherein the first promoter and the second promoter are separately selected from a CaMV 35S promoter, CaMV 19S promoter, nos promoter, Adh1 promoter, sucrose synthase promoter, a-tubulin promoter, ubiquitin promoter, actin promoter, cab promoter, PEPCase promoter, R gene complex promoter, poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter, cauliflower mosaic virus promoter, Z10 promoter from a gene encoding a 10 kDa zein protein, Z27 promoter from a gene encoding a 27 kDa zein protein, pea rbcS gene and the actin promoter from rice promoter, or phaseolin promoter.
7. A method comprising growing a plant seed or plant comprising an expression system comprising (a) a first expression cassette comprising a first promoter operably linked to a nucleic acid segment encoding an IRE1 polypeptide comprising an amino acid sequence with at least 95% sequence identity to any of SEQ ID NO: 9 and 11, 12, 13, 14, or 15; and (b) a second expression cassette comprising a second promoter operably linked to a nucleic acid segment encoding a CSLF6 polypeptide comprising an amino acid sequence with at least 95% sequence identity to any of SEQ ID NO: 1, 5, 6, 7, or 8, to thereby produce a mature plant, wherein the plant cell, plant seed, or plant are selected from the group consisting of Brachypodium distachyon , wheat, barely, corn, rice, or sorghum.
8. The method of claim 7 , further comprising harvesting biomass from the mature plant.
9. The method of claim 8 , further comprising isolating glucan, oligosaccharides, disaccharides, monosaccharides, or a combination thereof from the biomass.
10. The method of claim 8 , wherein the first promoter or the second promoter is a strong or inducible promoter.
11. The method of claim 8 , wherein the first promoter or the second promoter is a tissue-specific promoter.
12. The method of claim 7 , wherein the first promoter and the second promoter are separately selected from a CaMV 35S promoter, CaMV 19S promoter, nos promoter, Adh1 promoter, sucrose synthase promoter, a-tubulin promoter, ubiquitin promoter, actin promoter, cab promoter, PEPCase promoter, R gene complex promoter, poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter, cauliflower mosaic virus promoter, Z10 promoter from a gene encoding a 10 kDa zein protein, Z27 promoter from a gene encoding a 27 kDa zein protein, pea rbcS gene and the actin promoter from rice promoter, or phaseolin promoter.
13. The plant of claim 1 , wherein a population of the plants having the expression system has an average height that is the same as or at least 5% greater than an average height of a corresponding wild type population of plants of the same age, where the wild type population of plants does not have the expression system.
14. The method of claim 7 , wherein a population of the plants having the expression system has an average height that is the same as or at least 5% greater than an average height of a corresponding wild type population of plants of the same age, where the wild type population of plants does not have the expression system.