Granted Patent
B2
US 12,453,743 · App. 17/059,874 · Granted Oct 28, 2025
Mesenchymal stem cell (MSC) growth factor and extracellular vesicle preparation in frozen or powdered form and methods of use
Assignee:
DIRECT BIOLOGICS, LLC
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Abstract
Disclosed are mesenchymal stem cell growth factor compositions and methods of their use to treat skin disorder, alleviate the effects of aging, treat wounds, orthopedic disorders, sexual dysfunction and/or reduce inflammation.
Claims (11)
1 . A pharmaceutical acellular composition for human administration, wherein the pharmaceutical acellular composition comprises:
a therapeutically effective amount of a human bone marrow mesenchymal stem cell (MSC) secretome comprising epidermal growth factor (EGF), bone morphogenetic protein 1 (BMP-1), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and interleukin-6 (IL-6), wherein the therapeutically effective amount is sufficient to reduce inflammation in the human, wherein the pharmaceutical acellular composition is in a powdered form.
2 . The pharmaceutical acellular composition of claim 1 , wherein the bone marrow MSC secretome is secreted and separated from the human bone marrow MSC.
3 . A pharmaceutical solution comprising the pharmaceutical acellular composition of claim 1 and saline.
4 . The pharmaceutical acellular composition of claim 1 , wherein 0.01% to 10% by weight of the pharmaceutical acellular composition is the bone marrow MSC secretome.
5 . The pharmaceutical acellular composition of claim 1 , wherein the bone marrow MSC secretome comprises extracellular vesicles having a size of 35 nm to 200 nm.
6 . The pharmaceutical acellular composition of claim 5 , wherein the extracellular vesicles are 50 nm to 150 nm in size.
7 . The pharmaceutical solution of claim 3 , wherein the pharmaceutical solution is a frozen liquid.
8 . The pharmaceutical acellular composition of claim 1 , wherein the pharmaceutical acellular composition comprises sucrose, D-mannose, lactose, trehalose, or glucose, or a combination of two or more thereof.
9 . The pharmaceutical acellular composition of claim 1 , wherein the pharmaceutical acellular composition is sterilized.
10 . The pharmaceutical acellular composition of claim 1 , wherein the bone marrow MSC secretome further comprises insulin-like growth factor (IGF).
Assignments (4)
CORRECTIVE ASSIGNMENT TO CORRECT THE ATTORNEY DOCKET NO. PREVIOUSLY RECORDED ON REEL 72425 FRAME 272. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT.
Recorded Oct 20, 2025
From: MOSELEY, TIMOTHY ALEXANDER
To: DIRECT BIOLOGICS, LLC
Reel/Frame 073116/0865 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 30, 2025
From: MOSELEY, TIMOTHY ALEXANDER
To: DIRECT BIOLOGICS, LLC
Reel/Frame 072425/0272 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 24, 2025
From: PETTINE, KENNETH ALLEN
To: DIRECT BIOLOGICS, LLC
Reel/Frame 072925/0519 →
NUNC PRO TUNC ASSIGNMENT
Recorded Sep 1, 2021
From: PETTINE, KENNETH ALLEN
To: DIRECT BIOLOGICS LLC
Reel/Frame 057393/0691 →
Continuity (5)
Provisional Application
62698673
· Jul 16, 2018
Provisional Application
62688012
· Jun 21, 2018
Provisional Application
62681172
· Jun 6, 2018
Provisional Application
62677744
· May 30, 2018
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