Lyophilized formulation of stem cell-derived exosomes and anti-inflammatory composition including the same as active ingredient
The lyophilized formulation of stem cell-derived exosomes and the anti-inflammatory composition including the same as an active ingredient is able to stabilize stem cell-derived exosomes and exhibit excellent anti-inflammatory effects, and particularly, exhibit remarkable anti-inflammatory effects as compared with not-lyophilized stem cell-derived exosomes isolated and purified from conditioned media of stem cells. Therefore, the lyophilized formulation of stem cell-derived exosomes and the anti-inflammatory composition including the same as an active ingredient is able to effectively prevent, suppress, alleviate, ameliorate or treat inflammatory response or inflammatory diseases.
1. An anti-inflammatory composition comprising:
a lyophilized formulation of stem cell-derived exosomes comprising stem cell-derived exosomes, and a combination of methionine, mannitol and trehalose as a cryoprotectant; and
a diluent;
wherein the diluent comprises water for injection, physiological saline, phosphate buffered saline, purified water, or deionized water; and
wherein the diluent further comprises hyaluronic acid or hyaluronate.
2. The anti-inflammatory composition of claim 1 , wherein the composition is prepared as a suspension.
3. The anti-inflammatory composition of claim 1 , wherein the composition is a pharmaceutical composition, a cosmetic composition, or a skin external preparation.
4. The anti-inflammatory composition of claim 3 , wherein the pharmaceutical composition is an injectable formulation.
5. A method for alleviating, ameliorating or treating inflammation of skin of a subject in need thereof, the method comprising step of:
(a) treating an inflammatory area in the skin of the subject with an anti-inflammatory composition, wherein the anti-inflammatory composition comprises a lyophilized formulation of stem cell-derived exosomes which comprises stem cell-derived exosomes and a combination of methionine, mannitol and trehalose as a cryoprotectant.
6. The method of claim 5 , wherein the anti-inflammatory composition further comprises a diluent.
7. The method of claim 6 , wherein the diluent comprises water for injection, physiological saline, phosphate buffered saline, purified water, or deionized water.
8. The method of claim 7 , wherein the diluent further comprises hyaluronic acid or hyaluronate.
9. The method of claim 6 , wherein the anti-inflammatory composition is prepared as a suspension.
10. The method of claim 6 , wherein the anti-inflammatory composition is an injectable formulation.
11. The method of claim 6 , wherein the anti-inflammatory composition is administered to the skin by microneedling, iontophoresis, or injection.
12. The method of claim 5 , further comprising steps of:
(b) performing iontophoresis by allowing a microcurrent to flow through the skin treated with the anti-inflammatory composition; and
(c) delivering the anti-inflammatory composition inside the skin by the microcurrent.
13. The method of claim 12 , wherein the anti-inflammatory composition is used in at least one form selected from the group consisting of a patch, a mask pack, a mask sheet, a cream, a tonic, an ointment, a suspension, an emulsion, a paste, a lotion, a gel, an oil, a spray, an aerosol, a mist, a foundation, a powders, and an oilpaper.
14. The method of claim 13 , wherein the anti-inflammatory composition is applied to or soaked in at least one surface of the patch, the mask pack, or the mask sheet.
15. The method of claim 14 , wherein the step (a) is performed by:
(a1) applying the anti-inflammatory composition directly to the skin; or
(a2) contacting or attaching the mask pack, the mask sheet or the patch, which has the anti-inflammatory composition applied thereto or soaked therein, to the skin; or
sequentially performing (a1) and (a2).
16. The method of claim 15 , wherein the step (b) is performed by contacting or attaching an iontophoresis device to the skin.
17. The method of claim 16 , wherein the iontophoresis device comprises at least one battery selected from the group consisting of flexible batteries, lithium-ion secondary batteries, alkaline batteries, dry cells, mercury batteries, lithium batteries, nickel-cadmium batteries, and reverse electrodialysis batteries.
18. A method for alleviating, ameliorating or treating inflammation of skin of a subject in need thereof, the method comprising step of:
(a1) applying an anti-inflammatory composition to the skin of the subject;
or (a2) contacting or attaching a patch, a mask pack or a mask sheet, which has the anti-inflammatory composition applied thereto or soaked therein, to the skin; or (a3) sequentially performing (a1) and (a2),
wherein the anti-inflammatory composition comprises a lyophilized formulation of stem cell-derived exosomes which comprises stem cell-derived exosomes and a combination of methionine, mannitol and trehalose as a cryoprotectant.
19. The method of claim 18 , wherein the anti-inflammatory composition further comprises a diluent.
20. The method of claim 19 , wherein the diluent comprises water for injection, physiological saline, phosphate buffered saline, purified water, or deionized water.
21. The method of claim 20 , wherein the diluent further comprises hyaluronic acid or hyaluronate.
22. The method of claim 18 , wherein the anti-inflammatory composition is prepared as a suspension.
23. The method of claim 18 , further comprising step (b) removing the patch, the mask pack or the mask sheet from the skin after step (a2) or (a3), and applying the anti-inflammatory composition to the skin.
24. A method for alleviating, ameliorating or treating inflammation of skin of a subject in need thereof, the method comprising
administering a therapeutically effective amount of an anti-inflammatory composition to the subject, wherein the anti-inflammatory composition comprises a lyophilized formulation of stem cell-derived exosomes which comprises stem cell-derived exosomes and a combination of methionine, mannitol and trehalose as a cryoprotectant.
25. The method of claim 24 , wherein the anti-inflammatory composition further comprises a diluent.
26. The method of claim 25 , wherein the diluent comprises water for injection, physiological saline, phosphate buffered saline, purified water, or deionized water.
27. The method of claim 26 , wherein the diluent further comprises hyaluronic acid or hyaluronate.
28. The method of claim 24 , wherein the anti-inflammatory composition is prepared as a suspension.