Patent
The present disclosure relates to the field of plant breeding and, more specifically, to the development of white rust ( Albugo occidentalis ) resistant and downy mildew ( Peronospora farinosa f. sp. spinaceae) resistant spinach plants having elite agronomic traits. The resistance to white rust is conferred by one or more alleles that co-segregate with at least one molecular marker selected from the group of SEQ ID No. 1-10. The resistance to downy mildew is conferred by an allele that co-segregates with at least one molecular marker selected from the group of SEQ ID No. 11-13. The disclosure relates further to the use of the molecular markers.
1. A cultivated spinach ( Spinacia oleracea L.) plant, wherein said plant is resistant to white rust caused by the plant pathogen Albugo occidentalis (Ao), wherein said resistance is provided by one or more resistance alleles conferring resistance to said plant pathogen, and wherein said alleles co-segregate with at least one molecular marker selected from the group of SEQ ID NO: 1 comprising a T to C SNP at nucleotide 101, SEQ ID NO: 2 comprising a T to A SNP at nucleotide 101, SEQ ID NO: 3 comprising an A to G SNP at nucleotide 101, SEQ ID NO: 4 comprising a G to A SNP at nucleotide 101, SEQ ID NO: 5 comprising a T to C SNP at nucleotide 101 and a G to A SNP at nucleotide 158, SEQ ID No. SEQ ID NO: 6 comprising a C to A SNP at nucleotide 101, SEQ ID NO: 7 comprising a G to T SNP at nucleotide 101, SEQ ID NO: 8 comprising a C to T SNP at nucleotide 101, SEQ ID NO: 9 comprising a C to T SNP at nucleotide 101, and SEQ ID NO: 10 comprising an A to G SNP at nucleotide 101.
2. The cultivated spinach plant of claim 1 , wherein the Ao resistance is conferred by two or more resistance alleles comprising:
(a) a first resistance allele located on chromosome 1, wherein said allele co-segregates with at least one molecular marker selected from the group of SEQ ID NO: 1 comprising the T to C SNP at nucleotide 101, SEQ ID NO: 2 comprising the T to A SNP at nucleotide 101, and SEQ ID NO: 3 comprising the A to G SNP at nucleotide 101;
(b) a second resistance allele located on chromosome 2, wherein said allele co-segregates with at least one molecular marker selected from the group of SEQ ID NO: 4 comprising the G to A SNP at nucleotide 101, SEQ ID NO: 5 comprising the T to C SNP at nucleotide 101 and the G to A SNP at nucleotide 158, and SEQ ID NO: 6 comprising the C to A SNP at nucleotide 101; and
(c) a third resistance allele located on chromosome 3, wherein said allele co-segregates with at least one molecular marker selected from the group of SEQ ID NO: 7 comprising the G to T SNP at nucleotide 101, SEQ ID NO: 8 comprising the C to T SNP at nucleotide 101, SEQ ID NO: 9 comprising the C to T SNP at nucleotide 101, and SEQ ID NO: 10 comprising the A to G SNP at nucleotide 101.
3. The cultivated spinach plant of claim 2 , wherein the first resistance allele, the second resistance allele, and the third resistance allele are present in the plant in homozygous state.
4. The cultivated spinach plant of claim 1 , wherein said plant is further resistant to downy mildew caused by the plant pathogen Peronospora farinosa f sp. spinaciae (Pfs), wherein the resistance to Pfs is to at least Pfs races 1 to 15 and race 17, and wherein said resistance is provided by a fourth resistance allele conferring resistance to said plant pathogen.
5. The cultivated spinach plant of claim 4 , wherein said fourth resistance allele is located on chromosome 3, and wherein said fourth resistance allele co-segregates with at least one molecular marker selected from the group of SEQ ID NO: 11 comprising an A to G SNP at nucleotide 101, SEQ ID NO: 12 comprising a G to A SNP at nucleotide 101, and SEQ ID NO: 13 comprising a T to C SNP at nucleotide 101.
6. The cultivated spinach plant of claim 5 , wherein the fourth resistance allele is present in the plant in homozygous state.
7. A propagating material derived from the cultivated spinach plant of claim 1 , wherein said propagating material is selected from the group of stems, cuttings, petioles, hypocotyls, cotyledons, flowers, anthers, pollen, ovaries, roots, root tips, protoplasts, callus, microspores, stalks, ovules, shoots, seeds, embryos, embryo sacs, egg cells, cells, meristems, buds, and leaves, and said propagating material is capable of growing into the cultivated spinach plant of claim 1 .
8. A progeny plant of the cultivated spinach plant of claim 1 , wherein said progeny plant retains the one or more resistance alleles which confer resistance to Ao.
9. A progeny plant of the cultivated spinach plant of claim 4 , wherein said progeny plant retains the resistance alleles which confer resistance to Ao and Pfs.
10. A tissue culture comprising regenerable cells from the propagating material of claim 7 .
11. A cultivated spinach plant regenerated from the tissue culture of claim 10 , wherein said plant retains the one or more resistance alleles which confer resistance to Ao.
12. A tissue culture of the cultivated spinach plant of claim 4 .
13. A cultivated spinach plant regenerated from the tissue culture of claim 12 , wherein said plant retains one or more of the resistance alleles which confer resistance to Ao and Pfs.
14. A cultivated spinach plant according to claim 1 , said plant produced by growing seed of inbred spinach variety designated as ‘X17-003-104-8’, representative sample of seed having been deposited under NCIMB Accession Number 43477.
15. The cultivated spinach plant according to claim 4 , wherein the plant was produced by growing seed of inbred spinach variety designated as ‘X17-003-104-8’, representative sample of seed having been deposited under NCIMB Accession Number 43477.
16. The seed of inbred spinach variety designated as ‘X17-003-104-8’, representative sample of seed having been deposited under NCIMB Accession Number 43477.
17. A method of generating a cultivated spinach plant comprising resistance to Ao or Ao and Pfs, said method comprising the steps:
(a) crossing the spinach plant produced by growing the seeds deposited under NCIMB Accession Number 43477 with itself or with a second spinach plant; and
(b) selecting an F1 progeny plant comprising resistance to Ao or an F1 progeny plant comprising resistance to Ao and Pfs.
18. The method of claim 17 , further comprising the steps:
(c) crossing the F1 progeny plant with a second spinach plant to produce backcross progeny plants; and
(d) selecting a backcross progeny plant comprising resistance to Ao or a backcross progeny plant comprising resistance to Ao and Pfs.
19. A method of identifying the resistant spinach plant of claim 1 , said method comprising the identification of one or more molecular markers selected from the group of the resistance alleles of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10.
20. A method of identifying the resistant spinach plant of claim 5 , said method comprising the identification of one or more molecular markers selected from the group of the resistant alleles of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, and SEQ ID NO: 13.
21. A spinach plant comprising the one or more molecular markers identified by the method of claim 19 .
22. A spinach plant comprising the one or more molecular markers identified by the method of claim 20 .
23. A propagating material derived from the cultivated spinach plant of claim 4 , wherein said propagating material is selected from one or more of stems, cuttings, petioles, hypocotyls, cotyledons, flowers, anthers, pollen, ovaries, roots, root tips, protoplasts, callus, microspores, stalks, ovules, shoots, seeds, embryos, embryo sacs, egg cells, cells, meristems, buds, and leaves, and said propagating material is capable of growing into the cultivated spinach plant of claim 4 .
24. A cell culture comprising regenerable cells from the propagating material of claim 23 .