Microfluidic microchips by 3D printing
Function fabrication in a microfluidic device manufactured with a custom 3D printer. The functions may include, for example, transporting or routing fluid, fluid mixing through flow and/or diffusion, blocking fluid (valve), pumping fluid, providing chemical reaction regions, providing analyte capture regions, and providing analyte separation regions. The fluid may be a liquid or a gas.
1. A microfluidic device comprising,
a structure of photo-cured resin,
the structure of multiple adjacent layers of photo-cured resin with each layer having one or more resin regions of preselected cross-linking
with multiple photo-cured resin layers of the photo-cured adjacent layers having one or more regions containing non-crosslinked resin where there is no crosslinking of resin, resulting in no resin or void regions when non-crosslinked resin is removed, such that void regions within multiple adjacent layers are disposed to provide a three-dimensional network of channels configured for microfluidic function.
2. A device as in claim 1 wherein a wall of a channel is defined by one or more adjacent layers where resin in a second resin region adjacent to the void region defining the channel is crosslinked further or less than the adjacent crosslinked resin region.
3. A device as in claim 1 wherein the at least one layer has one or more regions with the same or different crosslinking to a region of an adjacent layer.
4. A device as in claim 1 wherein at least one layer has a different thickness to other layers in the device.
5. A device as in claim 4 wherein the at least one layer with a different thickness has one or more regions with the same or different crosslinking to a region of other layers in the device.
6. A device as in claim 4 wherein the at least one layer is thinner than overlying adjacent layers on both surfaces and the at least one layer has a cured resin region adjacent to a void region in both of the adjacent layers to provide a flexible membrane between first and second channels provided by the no resin regions.
7. A device as in claim 6 wherein the membrane functions as a membrane pump wherein an oscillating pressure in first channel provides a pumping action in the second channel.
8. A device as in claim 6 wherein the flexible membrane is activated by a change in pressure in the first channel, and the activation switches a function in the second channel.
9. A device as in claim 8 wherein the function in the second channel is switching between blocking the second channel and unblocking the second channel.
10. A device as in claim 9 wherein the function functions by change in pressure in the first channel to selectively extend the membrane into the second channel sufficient to block the second channel and selectively withdrawing the membrane from the second channel to unblock the second channel.
11. A device as in claim 1 wherein a layer on at least one of its two surfaces, has an overlying adjacent layer that does not extend over all of its at least one surface.
12. A device as in claim 11 wherein on its at least one surface has at least two overlying adjacent layers, with at least one of the adjacent layers having a thickness different than other overlying adjacent layers on the at least one surface.
13. A device as in claim 12 wherein the at least one of the overlying adjacent layers is thinner than the other overlying adjacent layers on the at least one surface, and the thinner adjacent layer is duplicated for several new layers forming a stack of thinner layers.
14. A device as in claim 13 wherein a wall of the channel is defined by the stack of thinner layers being adjacent to void regions of the layers.
15. A device as in claim 1 wherein above layers are above a void region, the above layers crosslinked to a degree that during fabrication reduce the UV light from UV doses being applied above for new curing layers to reduce crosslinking to uncured resin in the void region.
16. A device as in claim 1 wherein the device comprises a truly 3D network of interconnecting channels of different functions.
17. A device as in claim 16 wherein a function of a channel is one or more function selected from transporting or routing fluid, fluid mixing through flow and/or diffusion, blocking fluid (valve), pumping fluid, providing chemical reaction regions, providing analyte capture regions, and providing analyte separation regions, where the fluid is a liquid or a gas.
18. A device as in claim 1 having at least one channel for mixing two or more liquids, with a network of channels to transport the liquids to and withdraw the mixed liquids from the at least one channel.
19. A device as in claim 18 wherein the at least one channel for mixing liquids has a dilution function for diluting a first liquid with a second liquid.
20. A device as in claim 19 wherein there are two or more channels for mixing liquid with a dilution function, the two or more channels are interconnected to provide a serial dilution.