The present disclosure concerns methods of using novel bacterial strains of 0617-T307, 0917-T305, 0917-T306, 0917-T307, 0118-T319, 0318-T327, and 0418-T328, the cell broth and novel metabolites produced from the bacterial strains, that can inhibit the growth of a variety of microbial species for a variety of crops. The methods include use of novel, potent antimicrobial metabolites produced from the strains corresponding to compounds having Formulas (I), (II), and (III):
1. A method of controlling a bacterial crop disease, comprising the steps of
(i) producing an agricultural composition comprising:
(a) a bacterial fermentate produced from a Pseudomonas bacteria selected from the group consisting of Pseudomonas soli 0617-T307 (Accession No. PTA-126796), Pseudomonas soli 0917-T305 (Accession No. PTA-126797), Pseudomonas soli 0917-T306 (Accession No. PTA-126798), Pseudomonas soli 0917-T307 (Accession No. PTA-126799), Pseudomonas mosselii 0118-T319 (Accession No. PTA-126800), Pseudomonas mosselii 0318-T327 (Accession No. PTA-126801), and Pseudomonas mosselii 0418-T328 (Accession No. PTA-126802); or a protective supernatant derived therefrom; or
(b) an agriculture composition comprising at least one of Formula (I) or Formula (II):
and
(ii) applying said agricultural composition a crop to inhibit the growth of a pathogenic microorganism.
2. The method of 1 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
3. The method of claim 1 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.
4. The method according to any of claims 1 - 3 , wherein the crop is selected from the group consisting of bananas, apples, pears, crabapples, citrus, potatoes, pumpkins, onions, rice, African violets, plant species of Cruciferae, Solanaceae, Cucurbitaceae including carrots, potatoes, tomatoes, eggplants, leafy greens, squashes and cucurbits, peppers and green peppers, olives, stone and pome fruit plants including olives, peaches, and walnuts.
5. A method of controlling a bacterial crop disease, comprising:
applying an agricultural composition comprising between about 1.0×10 5 and 1.0×10 9 cfu per mL Pseudomonas bacteria to a crop to inhibit the growth of a pathogenic microorganism,
wherein the Pseudomonas bacteria is selected from the group consisting of Pseudomonas soli and Pseudomonas mosselii.
6. The method of claim 5 , wherein the Pseudomonas bacteria is selected from the group consisting of Pseudomonas soli 0617-T307 (Accession No. PTA-126796), Pseudomonas soli 0917-T305 (Accession No. PTA-126797), Pseudomonas soli 0917-T306 (Accession No. PTA-126798), Pseudomonas soli 0917-T307 (Accession No. PTA-126799), Pseudomonas mosselii 0118-T319 (Accession No. PTA-126800), Pseudomonas mosselii 0318-T327 (Accession No. PTA-126801), and Pseudomonas mosselii 0418-T328 (Accession No. PTA-126802).
7. The method of claim 5 , wherein the composition comprises between about 5.0×10 7 and 2.0×10 8 cfu per mL Pseudomonas bacteria.
8. The method of claim 5 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
9. The method according to any of claims 5 - 8 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.
10. The method according to any of claims 5 - 8 , wherein the crop is selected from the group consisting of bananas, apples, pears, crabapples, citrus, potatoes, pumpkins, onions, rice, African violets, plant species of Cruciferae, Solanaceae, Cucurbitaceae including carrots, potatoes, tomatoes, eggplants, leafy greens, squashes and cucurbits, peppers and green peppers, olives, stone and pome fruit plants including olives, peaches, and walnuts.
11. A method of a controlling bacterial crop disease, comprising:
(i) producing an agricultural composition comprising a bacterial fermentate from Pseudomonas bacteria selected from the group consisting of Pseudomonas soli 0617-T307 (Accession No. PTA-126796), Pseudomonas soli 0917-T305 (Accession No. PTA-126797), Pseudomonas soli 0917-T306 (Accession No. PTA-126798), Pseudomonas soli 0917-T307 (Accession No. PTA-126799), Pseudomonas mosselii 0118-T319 (Accession No. PTA-126800), Pseudomonas mosselii 0318-T327 (Accession No. PTA-126801), and Pseudomonas mosselii 0418-T328 (Accession No. PTA-126802), and
(ii) applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism.
12. The method of 11 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
13. The method claim 11 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.
14. The method of claim 11 , wherein the pathogenic microorganism is a pathogenic E. amylovora being a streptomycin-resistant E. amylovora.
15. The method according to any of claims 11 - 14 , wherein the crop is selected from the group consisting of bananas, apples, pears, crabapples, citrus, potatoes, tomatoes, eggplants, leafy greens, squashes, cucurbits, peppers, green peppers, stone fruit, pome fruit, olives, peaches, and walnuts.
16. The method of claim 11 , wherein the pathogenic microorganism is selected from Flavobacterium columnare #2 and Flavobacterium columnare MS-FC-4.
17. The method of claim 11 , wherein the pathogenic microorganism is E. coli O157:H7.
18. The method of claim 11 , comprising:
applying said agricultural composition comprising between about 1.0×10 5 and 1.0×10 9 cfu per mL Pseudomonas bacteria to the crop to inhibit the growth of the pathogenic microorganism.
19. The method according to claim 18 , wherein the agricultural composition comprises between about 5.0×10 7 and 2.0×10 8 cfu per mL Pseudomonas bacteria.
20. A method of controlling a bacterial crop disease, comprising the steps of
(i) producing an agricultural composition comprising at least one of Formula (I) or Formula (II):
and
(ii) applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism.
21. The method of 20 , wherein the bacterial crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
22. The method of claim 20 , wherein the crop is selected from the group consisting of bananas, apples, pears, crabapples, citrus, potatoes, pumpkins, onions, rice, African violets, plant species of Cruciferae, plant species of Solanaceae , plant species of Cucurbitaceae , carrots, tomatoes, eggplants, leafy greens, squashes, cucurbits, peppers, green peppers, stone fruit, pome fruit, olives, peaches, and walnuts.
23. The method of claim 20 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasihensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.
24. The method of controlling the bacterial crop disease of claim 20 , wherein the agricultural composition comprises Formula (I):
25. The method of 24 , wherein the bacterial crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
26. The method of claim 24 , wherein the crop is selected from the group consisting of bananas, apples, pears, crabapples, citrus, potatoes, pumpkins, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae , carrots, tomatoes, eggplants, leafy greens, squashes, cucurbits, peppers, green peppers, stone fruit, pome fruit, olives, peaches, and walnuts.
27. The method of claim 24 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.