Methods for using mosaicism in nucleic acids sampled distal to their origin
Disclosed herein are methods for improving detection and monitoring of human diseases. The methods can be used to provide spatial and/or developmental localization of the source of each differential mutation within the body. The methods can also be used to generate a mutation map of a subject. And the mutation map can be used to monitoring state(s) of health of one or more tissues of a subject.
1. A method for identifying mosaic variants specific to a subject from a non-fetal source in a blood sample from a subject, comprising:
(a) obtaining nucleic acid molecules from (i) a plasma fraction comprising cell-free nucleic acid molecules and (ii) a buffy coat fraction of said blood sample from said subject;
(b) assaying said nucleic acid molecules from said plasma fraction and said buffy coat fraction to generate sequence information comprising sequences derived from said plasma fraction and sequences derived from said buffy coat fraction, wherein said assaying comprises whole genome sequencing;
(c) subsequent to (b), identifying mosaic variants specific to said subject in said sequences derived from said plasma fraction by subtracting out a blood-specific signal of said sequences derived from said buffy coat fraction, wherein said sequences derived from said nucleic acid molecules of said buffy coat fraction are a normal; and
(d) providing a report based on an identification of said mosaic variants specific to said subject.
2. The method of claim 1 , further comprising obtaining an additional blood or plasma sample at a later time point, repeating (a)-(c), and reporting changes in said mosaic variants specific to said subject.
3. The method of claim 2 , wherein said changes are a quantified signal of a variant from said mosaic variants specific to said subject.
4. The method of claim 2 , wherein said mosaic variants specific to said subject are identified using capture probes in said additional blood or plasma sample.
5. The method of claim 1 , wherein said mosaic variants specific to said subject are selected from the group consisting of: copy number variation, small insertions and deletions (inDels), single nucleotide polymorphisms (SNPs), multiple nucleotide polymorphisms (MNPs), and any combination thereof.
6. The method of claim 1 , wherein said mosaic variants specific to said subject are causal or predisposing for a disease.
7. The method of claim 1 , wherein said mosaic variants specific to said subject are non-causal for a disease.
8. The method of claim 2 , further comprising enriching said nucleic acid molecules of said plasma fraction or said buffy coat fraction.
9. The method of claim 8 , further comprising enriching said nucleic acid molecules of said plasma fraction and said buffy coat fraction.
10. The method of claim 8 , wherein said nucleic acid molecules are enriched based on mean size, mean GC content, genomic regions, genomic region feature, or a combination thereof.
11. The method of claim 8 , wherein said enriching comprises using a capture probe that hybridizes to one or more of said nucleic acid molecules.
12. The method of claim 11 , wherein said capture probe is attached to an array or a bead.
13. The method of claim 1 , further comprising, prior to (b), amplifying said nucleic acid molecules.
14. The method of claim 1 , wherein said subject is suffering from cancer.
15. The method of claim 1 , wherein said subject is a human subject.
16. The method of claim 1 , wherein said blood sample is derived from a whole blood sample.
17. The method of claim 1 , wherein said cell-free nucleic acid molecules comprise deoxyribonucleic acids (DNA).
18. The method of claim 1 , wherein said buffy coat fraction comprises white blood cells.
19. The method of claim 1 , wherein said nucleic acid molecules of said buffy coat fraction are derived from leukocytes.
20. The method of claim 1 , further comprising providing a therapeutic intervention based on an identification of said mosaic variants specific to said subject.