Biomarkers for graft-versus-host disease
Biomarkers are provided that are predictive of a subject's responsiveness to a JAK inhibitor. The biomarkers, compositions, and methods described herein are useful in selecting appropriate treatment modalities for a subject having, suspected of having, or at risk of developing Graft-Versus-Host Disease.
1. A method of treating a human subject having, suspected of having, or at risk of developing Graft-Versus-Host Disease (GvHD), comprising administering to the human subject a JAK inhibitor, wherein the human subject has been previously determined to have (i) a baseline concentration of at least one protein selected from the group consisting of IL8, IL-24, IL6, AREG, CXCL1, G-CSF, CCL20, MMP-1, CXCL5, MMP12, CHRDL2, SIGLEC10, KRT19, CXCL6, RETN, CD177, MUC-16, TNFRSF6B, CKAP4, CHI3L1, ST2, TNFSF14, PGLYRP1, IL-4RA, SLAMF8, LCN2, CCL19, MFGE8, U-PAR, CEACAM8, CAIX, CLEC4D, CD163, TRAIL-R2, CLM-1, DPP10, NCF2, MMP-9, B4GALT1, PTPRJ, IL-1RT2, TMSB10, FSTL3, FGR, CCL3, CLEC7A, JAM-A, CAPG, TNF-R1, TFF2, VSIG4, SIGLEC1, HAVCR2, SELE, NUCB2, CXCL16, BTN3A2, GLRX, FUT3/FUT5, LILRB4, MSLN, MCP-1, CD38, DSC2, SLAMF7, PARP-1, IL-27, PCSK9, LAIR1, EPHA2, AGRP, ADAM 8, IL-18R1, OPG, IGFBP-2, VIM, TIMIP1, WISP-1, OPN, SEMA4C, ADM, DKK3, LTBR, TNFRSF10A, CLEC10A, PDGF-R-alpha, SHPS-1, TNFRSF14, NID2, VCAM1, ANGPTL1, LTA4H, WFDC2, IFN-gamma-R1, PTX3, COL1A1, NID1, NECTIN2, Siglec-9, EPHB4, CRIM1, MCFD2, and SPON2 in a biological sample obtained from the human subject that is lower than a control, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor, and/or (ii) a baseline concentration of at least one protein selected from the group consisting of NBL1, AOC3, PLTP, KIT, F11, TWEAK, NCAM1, CTSV, NTRK3, F7, CLUL1, TNFB, AGR3, TRANCE, APOM, FGF-BP1, CNTN5, CRTAC1, SERPINA9, CNDP1, GDF-8, sFRP-3, SCGB3A1, CGA, GCG, SCF, GAL, SERPINA5, PON3, CTRC, REG4, CCL25, and FABP2 in a biological sample obtained from the human subject that is higher than a control, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor.
2. The method of claim 1 , comprising administering to the human subject a JAK inhibitor, wherein the human subject has been previously determined to have (i) a baseline concentration of at least one protein selected from the group consisting of IL8, IL-24, IL6, AREG, CXCL1, G-CSF, CCL20, MMP-1, CXCL5, MMP12, CHRDL2, SIGLEC10, KRT19, CXCL6, RETN, CD177, MUC-16, TNFRSF6B, CKAP4, CHI3L1, ST2, TNFSF14, PGLYRP1, IL-4RA, SLAMF8, LCN2, CCL19, MFGE8, U-PAR, CEACAM8, CAIX, CLEC4D, CD163, TRAIL-R2, CLM-1, DPP10, NCF2, MMP-9, B4GALT1, PTPRJ, IL-1RT2, TMSB10, FSTL3, and FGR in a biological sample obtained from the human subject that is lower than a control, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor, and/or (ii) a baseline concentration of at least one protein selected from the group consisting of CGA, GCG, SCF, GAL, SERPINA5, PON3, CTRC, REG4, CCL25, and FABP2 in a biological sample obtained from the human subject that is higher than a control, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor.
3. A method of treating a human subject having, suspected of having, or at risk of developing Graft-Versus-Host Disease (GvHD), comprising:
providing a biological sample obtained from the human subject;
measuring in the biological sample: (i) a reduced concentration, as compared to a control, of at least one protein selected from the group consisting of IL8, IL-24, IL6, AREG, CXCL1, G-CSF, CCL20, MMP-1, CXCL5, MMP12, CHRDL2, SIGLEC10, KRT19, CXCL6, RETN, CD177, MUC-16, TNFRSF6B, CKAP4, CHI3L1, ST2, TNFSF14, PGLYRP1, IL-4RA, SLAMF8, LCN2, CCL19, MFGE8, U-PAR, CEACAM8, CAIX, CLEC4D, CD163, TRAIL-R2, CLM-1, DPP10, NCF2, MMP-9, B4GALT1, PTPRJ, IL-1RT2, TMSB10, FSTL3, FGR, CCL3, CLEC7A, JAM-A, CAPG, TNF-R1, TFF2, VSIG4, SIGLEC1, HAVCR2, SELE, NUCB2, CXCL16, BTN3A2, GLRX, FUT3/FUT5, LILRB4, MSLN, MCP-1, CD38, DSC2, SLAMF7, PARP-1, IL-27, PCSK9, LAIR1, EPHA2, AGRP, ADAM 8, IL-18R1, OPG, IGFBP-2, VIM, TIMIP1, WISP-1, OPN, SEMA4C, ADM, DKK3, LTBR, TNFRSF10A, CLEC10A, PDGF-R-alpha, SHPS-1, TNFRSF14, NID2, VCAM1, ANGPTL1, LTA4H, WFDC2, IFN-gamma-R1, PTX3, COL1A1, NID1, NECTIN2, Siglec-9, EPHB4, CRIM1, MCFD2, and SPON2, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with a JAK inhibitor, and/or (ii) an increased concentration, as compared to a control, of at least one protein selected from the group consisting of NBL1, AOC3, PLTP, KIT, F11, TWEAK, NCAM1, CTSV, NTRK3, F7, CLUL1, TNFB, AGR3, TRANCE, APOM, FGF-BP1, CNTN5, CRTAC1, SERPINA9, CNDP1, GDF-8, sFRP-3, SCGB3A1, CGA, GCG, SCF, GAL, SERPINA5, PON3, CTRC, REG4, CCL25, and FABP2, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with a JAK inhibitor; and
after the measuring, administering a JAK inhibitor to the human subject.
4. The method of claim 3 , comprising:
providing a biological sample obtained from the human subject;
measuring in the biological sample: (i) a reduced concentration, as compared to a control, of at least one protein selected from the group consisting of IL8, IL-24, IL6, AREG, CXCL1, G-CSF, CCL20, MMP-1, CXCL5, MIMP12, CHRDL2, SIGLEC10, KRT19, CXCL6, RETN, CD177, MUC-16, TNFRSF6B, CKAP4, CHI3L1, ST2, TNFSF14, PGLYRP1, IL-4RA, SLAMF8, LCN2, CCL19, MFGE8, U-PAR, CEACAM8, CAIX, CLEC4D, CD163, TRAIL-R2, CLM-1, DPP10, NCF2, MMP-9, B4GALT1, PTPRJ, IL-1RT2, TMSB10, FSTL3, and FGR, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor, and/or (ii) an increased concentration, as compared to a control, of at least one protein selected from the group consisting of CGA, GCG, SCF, GAL, SERPINA5, PON3, CTRC, REG4, CCL25, and FABP2, wherein the control is the concentration of the at least one protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor; and
after the measuring, administering the JAK inhibitor to the human subject.
5. The method of claim 1 , wherein a second therapeutic agent is administered to the human subject in combination with the JAK inhibitor.
6. The method of claim 5 , wherein the second therapeutic agent is a corticosteroid, methotrexate, cyclosporine, mycophenolate mofetil, tacrolimus, sirolimus, everolimus, antithymocyte globulin, alemtuzumab, cyclophosphamide, ibrutinib, imatinib, infliximab, etanercept, tocilizumab, basiliximab, daclizumab, rituximab, denileukin diftitox, pentostatin, thalidomide, halofuginone, hydroxychloroquine, or mesenchymal stem cells.
7. The method of claim 5 , wherein the second therapeutic agent is a corticosteroid.
8. The method of claim 7 , wherein the corticosteroid is methylprednisolone or prednisone.
9. The method of claim 1 , wherein the control is a pre-established cut-off value.
10. A method of treating a human subject having, suspected of having, or at risk of developing Graft-Versus-Host Disease (GvHD), comprising:
measuring, in a first biological sample obtained from the human subject prior to administering a JAK inhibitor, the concentration of at least one protein selected from the group consisting of EPO, THPO, GCG, FGF-21, Flt3L, CCL28, FABP4, FAM3B, IL6, FABP2, CCL25, VWC2, FAM19A5, PLIN1, MCP-1, NPPC, SCF, FBP1, SMOC2, TSHB, hK14, IL8, CD8A, PPY, CPA2, CHRDL2, BMP-6, FAM3C, TNNI3, MK, ADAM-TS 15, MMP12, CEACAM5, CXCL16, hK11, SLITRK2, GHRL, KAZALD1, Notch 3, CLMP, TFPI-2, GDF-2, GAL, Ep-CAM, IGFBP-1, GDF-8, CTRC, LEP, GDF-15, ROBO2, FR-alpha, KIM1, Gal-3, APLP1, CST5, CCL11, CX3CL1, RAGE, CD70, MSLN, FGF-23, SPINK1, DKK3, REN, VEGFA, CYR61, ITGB6, MMP7, CCL20, TGF-alpha, TN-R, SERPINA12, CADM3, RSPO3, MUC-16, CDON, TNFRSF19, RGMB, TMPRSS15, TACSTD2, SCGB3A2, CNTN1, IGF1R, DDC, NTF4, SCARF2, MYOC, SORCS2, MOG, RORI, VSIG2, LTBP2, ITGB5, ADAM 22, EDA2R, SCGB3A1, CA6, hK8, PSIP1, AMN, NCAM1, COLEC12, AGR2, P4HB, MAD homolog 5, CA12, DNER, TMPRSS5, LPL, GDNFR-alpha-3, GT, VEGFD, JAM-B, TGFBR3, ADAM 23, PDGFC, XG, TNFRSF13B, PON3, LAMA4, MSMB, TNFRSF4, PLC, TFF3, uPA, B4GAT1, DKKL1, ST3GAL1, WNT9A, LRP11, MMP-2, SEZ6L2, RARRES2, OPG, AGRP, SERPINA9, TF, GPNMB, PCSK9, INHBC, PAM, CLECIA, BMP-4, BCAN, TNFRSF12A, GIF, CES2, STC1, LRRN1, TWEAK, ADM, METRNL, CD200, CD83, SKR3, CD109, TRAIL, ACAN, CCL3, BOC, BCL2L11, PDGFRB, PRTG, SEZ6L, MDGA1, FLRT2, NTRK2, NPTXR, DRAXIN, OMD, COL1A1, CXL17, AGR3, FUT3/FUT5, WFIKKN2, NPDC1, ERBB4, TNFSF13B, CCL15, GALNT2, NTRK3, CCL16, GDNF, Gal-4, VIM, KLK13, MMP-10, SCARB2, CD300LG, IGSF3, NCAN, IL1RL2, Gal-1, ANGPTL3, PCDH17, UNC5C, LOX-1, NUCB2, CNTN2, TR-AP, MASP1, BCAM, NT-3, CCDC80, LAYN, AMBP, DLK-1, KLK10, GPC1, ENAH, CLUL1, XPNPEP2, CTSL1, GCP5, DCN, COMP, MIA, CNTN5, FCRLB, IGF2R, TRAIL-R2, CRHBP, Dkk-4, SPINT1, AP-N, MMP-3, B4GALT1, FGF-5, SSC4D, ERBB2, CRISP2, PAMR1, CNTN4, MERTK, PRELP, ENTPD6, EDIL3, PREB, TGFBI, ITGAV, SAA4, CLEC14A, IGFBPL1, MFAP5, PLA2G7, U-PAR, CANT1, PEAR1, EGFR, ST6GAL1, PODXL, TGFR-2, VASN, CD58, EFNA4, VEGFR-3, TFPI, CSTB, ROBO1, KLK6, LGMN, OPN, TNFRSFIOA, BLM hydrolase, CFC1, TIE2, QPCT, TYRO3, DDR1, NRP2, TREM1, ENTPD5, DPP6, CD99L2, AXL, MATN2, CPE, FURIN, S100A11, CPXM1, CDHR5, IL7, PDGF-R-alpha, FSTL3, HB-EGF, KLK12, PGF, F7, CTSD, OPTC, IFN-gamma-R1, PD-L2, HO-1, TNFRSF21, SPINT2, EZR, EDAR, SPON2, Alpha-2-MRAP, CD4, APOM, ESAM, ARSA, IL-17D, LRIG1, SEMA3F, EPHB6, CTSH, SCARA5, ALCAM, WFDC2, IL7R, SORT1, VEGFR-2, WISP-1, NOV, F11, LTBR, GALNT10, PIgR, DLL1, SPINK5, CLECIA, ERBB3, CD164, CKAP4, PODXL2, PTK7, PTH1R, ENPP2, GFRA2, BST1, CD97, MRC2, TIEl, NOMO1, ITGB1, UMOD, IGFBP-7, EPHA2, SHPS-1, BAMBI, SEMA4C, CDH5, CSF-1, NID1, ICAM-2, MARCO, LAP TGF-beta-1, AMIGO2, TNFSF13, PAR-1, IL-17RA, TM, SOD2, CD59, ADAM-TS13, CRIM1, PVR, CEACAM1, CTSS, Nr-CAM, PRKRA, GSAP, hOSCAR, STX16, TCN2, PSMA1, CD48, CLSTN2, GCNT1, SIGLEC1, TRIM5, CD244, SOST, CD200R1, TXLNA, CRX, ITGB2, ICAi, FOXO1, LY9, ERBB2IP, TLT-2, FCGR2A, IL-5R-alpha, FCGR3B, CD74, TPSABI, GP6, SCAMP3, PGLYRP1, LAG3, SELP, IRF9, PTX3, FASLG, MESDC2, INPPL1, TYMP, MBL2, PAPPA, SNAP23, NEMO, TRANCE, AHCY, ARHGEF12, SNAP29, IRAK4, HCLS1, CA13, CRKL, PRDX5, YES1, CEACAM8, AXIN1, GRAP2, CD84, ICAM3, VSIG4, THBS4, CRTAM, PDCD1, PIK3AP1, NUB1, SLAMF8, DCTN1, CASP-3, IL-24, PPP1R9B, LAT2, DDX58, FKBPlB, MAP4K5, MMP-9, CD27, ITGB1BP2, SELL, AREG, KLRD1, ANG-1, CCL5, CCL17, CD6, SKAP1, EGF, DAPP1, LAT, CD5, SH2D1A, TNC, CD69, ZBTB16, CXCL11, TANK, CD2AP, LAIR-2, ST1A1, STK4, CXCL9, GZMB, GZMH, SIT1, BANK1, CXCL10, RNASE3, PDGF subunit B, and IL2-RA;
administering the JAK inhibitor to the human subject; and
measuring, in a second biological sample obtained from the human subject after administering the JAK inhibitor, a reduced concentration, as compared to the concentration measured in the first biological sample, of at least one protein selected from the group consisting of PRKRA, GSAP, hOSCAR, STX16, TCN2, PSMA1, CD48, CLSTN2, GCNT1, SIGLEC1, TRIM5, CD244, SOST, CD200R1, TXLNA, CRX, ITGB2, ICA1, FOXO1, LY9, ERBB2IP, TLT-2, FCGR2A, IL-5R-alpha, FCGR3B, CD74, TPSAB1, GP6, SCAMP3, PGLYRP1, LAG3, SELP, IRF9, PTX3, FASLG, MESDC2, INPPL1, TYMP, MBL2, PAPPA, SNAP23, NEMO, TRANCE, AHCY, ARHGEF12, SNAP29, IRAK4, HCLS1, CA13, CRKL, PRDX5, YES1, CEACAM8, AXIN1, GRAP2, CD84, ICAM3, VSIG4, THBS4, CRTAM, PDCD1, PIK3AP1, NUB1, SLAMF8, DCTN1, CASP-3, IL-24, PPP1R9B, LAT2, DDX58, FKBPlB, MAP4K5, MMP-9, CD27, ITGB1BP2, SELL, AREG, KLRD1, ANG-1, CCL5, CCL17, CD6, SKAP1, EGF, DAPP1, LAT, CD5, SH2D1A, TNC, CD69, ZBTB16, CXCL11, TANK, CD2AP, LAIR-2, ST1A1, STK4, CXCL9, GZMB, GZMH, SIT1, BANK1, CXCL10, RNASE3, PDGF subunit B, and IL2-RA, and/or an increased concentration, as compared to the concentration measured in the first biological sample, of at least one protein selected from the group consisting of EPO, THPO, GCG, FGF-21, Flt3L, CCL28, FABP4, FAM3B, IL6, FABP2, CCL25, VWC2, FAM19A5, PLIN1, MCP-1, NPPC, SCF, FBP1, SMOC2, TSHB, hK14, IL8, CD8A, PPY, CPA2, CHRDL2, BMP-6, FAM3C, TNNI3, MK, ADAM-TS 15, MMP12, CEACAM5, CXCL16, hK11, SLITRK2, GHRL, KAZALD1, Notch 3, CLMP, TFPI-2, GDF-2, GAL, Ep-CAM, IGFBP-1, GDF-8, CTRC, LEP, GDF-15, ROBO2, FR-alpha, KIM1, Gal-3, APLP1, CST5, CCL11, CX3CL1, RAGE, CD70, MSLN, FGF-23, SPINKI, DKK3, REN, VEGFA, CYR61, ITGB6, MMP7, CCL20, TGF-alpha, TN-R, SERPINA12, CADM3, RSPO3, MUC-16, CDON, TNFRSF19, RGMB, TMPRSS15, TACSTD2, SCGB3A2, CNTN1, IGF1R, DDC, NTF4, SCARF2, MYOC, SORCS2, MOG, ROR1, VSIG2, LTBP2, ITGB5, ADAM 22, EDA2R, SCGB3A1, CA6, hK8, PSIP1, AMN, NCAM1, COLECi2, AGR2, P4HB, MAD homolog 5, CA12, DNER, TMPRSS5, LPL, GDNFR-alpha-3, GT, VEGFD, JAM-B, TGFBR3, ADAM 23, PDGFC, XG, TNFRSF13B, PON3, LAMA4, MSMB, TNFRSF4, PLC, TFF3, uPA, B4GAT1, DKKL1, ST3GAL1, WNT9A, LRP11, MMP-2, SEZ6L2, RARRES2, OPG, AGRP, SERPINA9, TF, GPNMB, PCSK9, INHBC, PAM, CLECI1A, BMP-4, BCAN, TNFRSF12A, GIF, CES2, STC1, LRRN1, TWEAK, ADM, METRNL, CD200, CD83, SKR3, CD109, TRAIL, ACAN, CCL3, BOC, BCL2L11, PDGFRB, PRTG, SEZ6L, MDGA1, FLRT2, NTRK2, NPTXR, DRAXIN, OMD, COL1A1, CXL17, AGR3, FUT3/FUT5, WFIKKN2, NPDC1, ERBB4, TNFSF13B, CCL15, GALNT2, NTRK3, CCL16, GDNF, Gal-4, VIM, KLK13, MMP-10, SCARB2, CD300LG, IGSF3, NCAN, IL1RL2, Gal-1, ANGPTL3, PCDH17, UNC5C, LOX-1, NUCB2, CNTN2, TR-AP, MASP1, BCAM, NT-3, CCDC80, LAYN, AMBP, DLK-1, KLK10, GPC1, ENAH, CLUL1, XPNPEP2, CTSL1, GCP5, DCN, COMP, MIA, CNTN5, FCRLB, IGF2R, TRAIL-R2, CRHBP, Dkk-4, SPINT1, AP-N, MMP-3, B4GALT1, FGF-5, SSC4D, ERBB2, CRISP2, PAMR1, CNTN4, MERTK, PRELP, ENTPD6, EDIL3, PREB, TGFBI, ITGAV, SAA4, CLEC14A, IGFBPL1, MFAP5, PLA2G7, U-PAR, CANT1, PEAR1, EGFR, ST6GAL1, PODXL, TGFR-2, VASN, CD58, EFNA4, VEGFR-3, TFPI, CSTB, ROBO1, KLK6, LGMN, OPN, TNFRSFIOA, BLM hydrolase, CFC1, TIE2, QPCT, TYRO3, DDR1, NRP2, TREM1, ENTPD5, DPP6, CD99L2, AXL, MATN2, CPE, FURIN, S100A11, CPXM1, CDHR5, IL7, PDGF-R-alpha, FSTL3, HB-EGF, KLK12, PGF, F7, CTSD, OPTC, IFN-gamma-R1, PD-L2, HO-1, TNFRSF21, SPINT2, EZR, EDAR, SPON2, Alpha-2-MRAP, CD4, APOM, ESAM, ARSA, IL-17D, LRIG1, SEMA3F, EPHB6, CTSH, SCARA5, ALCAM, WFDC2, IL7R, SORT1, VEGFR-2, WISP-1, NOV, F11, LTBR, GALNT10, PIgR, DLL1, SPINK5, CLECIA, ERBB3, CD164, CKAP4, PODXL2, PTK7, PTH1R, ENPP2, GFRA2, BST1, CD97, MRC2, TIE1, NOMO1, ITGB1, UMOD, IGFBP-7, EPHA2, SHPS-1, BAMBI, SEMA4C, CDH5, CSF-1, NID1, ICAM-2, MARCO, LAP TGF-beta-1, AMIGO2, TNFSF13, PAR-1, IL-17RA, TM, SOD2, CD59, ADAM-TS13, CRIM1, PVR, CEACAM1, CTSS, and Nr-CAM.
11. The method of claim 10 , comprising:
measuring, in the first biological sample obtained from the human subject prior to administering the JAK inhibitor, the concentration of at least one protein selected from the group consisting of EPO, THPO, GCG, FGF-21, Flt3L, CCL28, FABP4, FAM3B, IL6, FABP2, CCL25, VWC2, FAM19A5, PLIN1, MCP-1, NPPC, SCF, FBP1, SMOC2, TSHB, hK14, IL8, CD8A, PPY, CPA2, CHRDL2, BMP-6, FAM3C, TNNI3, MK, ADAM-TS 15, MMP12, CEACAM5, CXCL16, hK11, SLITRK2, GHRL, KAZALD1, Notch 3, CLMP, TFPI-2, GDF-2, GAL, Ep-CAM, IGFBP-1, GDF-8, CTRC, LEP, GDF-15, ROBO2, FR-alpha, KIM1, Gal-3, TNC, CD69, ZBTB16, CXCL11, TANK, CD2AP, LAIR-2, ST1A1, STK4, CXCL9, GZMB, GZMH, SIT1, BANK1, CXCL10, RNASE3, PDGF subunit B, and IL2-RA;
administering the JAK inhibitor to the human subject; and
measuring, in the second biological sample obtained from the human subject after administering the JAK inhibitor, a reduced concentration, as compared to the concentration measured in the first biological sample, of at least one protein selected from the group consisting of TNC, CD69, ZBTB16, CXCL11, TANK, CD2AP, LAIR-2, ST1A1, STK4, CXCL9, GZMB, GZMH, SIT1, BANK1, CXCL10, RNASE3, PDGF subunit B, and IL2-RA, and/or an increased concentration, as compared to the concentration measured in the first biological sample, of at least one protein selected from the group consisting of EPO, THPO, GCG, FGF-21, Flt3L, CCL28, FABP4, FAM3B, IL6, FABP2, CCL25, VWC2, FAM19A5, PLIN1, MCP-1, NPPC, SCF, FBP1, SMOC2, TSHB, hK14, IL8, CD8A, PPY, CPA2, CHRDL2, BMP-6, FAM3C, TNNI3, MK, ADAM-TS 15, MMP12, CEACAM5, CXCL16, hK11, SLITRK2, GHRL, KAZALD1, Notch 3, CLMP, TFPI-2, GDF-2, GAL, Ep-CAM, IGFBP-1, GDF-8, CTRC, LEP, GDF-15, ROBO2, FR-alpha, KIM1, and Gal-3.
12. The method of claim 10 , wherein a second therapeutic agent is administered to the human subject in combination with the JAK inhibitor.
13. The method of claim 12 , wherein the second therapeutic agent is a corticosteroid, methotrexate, cyclosporine, mycophenolate mofetil, tacrolimus, sirolimus, everolimus, antithymocyte globulin, alemtuzumab, cyclophosphamide, ibrutinib, imatinib, infliximab, etanercept, tocilizumab, basiliximab, daclizumab, rituximab, denileukin diftitox, pentostatin, thalidomide, halofuginone, hydroxychloroquine, or mesenchymal stem cells.
14. The method of claim 12 , wherein the second therapeutic agent is a corticosteroid.
15. The method of claim 14 , wherein the corticosteroid is methylprednisolone or prednisone.
16. The method of claim 1 , wherein the biological sample is blood, serum, plasma, urine, spinal fluid, saliva, lacrimal fluid, or sweat.
17. The method of claim 1 , wherein the concentration of the at least one protein is measured by an immunological method.
18. The method of claim 17 , wherein the immunological method is selected from the group consisting of enzyme-linked immunosorbent assay, enzyme immunoassay, radioimmunoassay, chemiluminescent immunoassay, electrochemiluminescence immunoassay, latex turbidimetric immunoassay, latex photometric immunoassay, immuno-chromatographic assay, and western blotting.
19. The method of claim 1 , wherein the concentration of the at least one protein is measured by mass spectrometry.
20. The method of claim 1 , wherein the JAK inhibitor is ruxolitinib.
21. The method of claim 1 , wherein the JAK inhibitor is itacitinib, 4-[3-(cyanomethyl)-3-(3′,5′-dimethyl-1H,1′H-4,4′-bipyrazol-1-yl)azetidin-1-yl]-2,5-difluoro-N-[(1S)-2,2,2-trifluoro-1-methylethyl]benzamide or a pharmaceutically acceptable salt thereof, or ((2R,5S)-5-{2-[(1R)-1-hydroxyethyl]-1H-imidazo[4,5-d]thieno[3,2-b]pyridin-1-yl}tetrahydro-2H-pyran-2-yl)acetonitrile or a pharmaceutically acceptable salt thereof.
22. The method of claim 1 , wherein the GvHD is acute GvHD.
23. The method of claim 1 , wherein the GvHD is chronic GvHD.
24. The method of claim 1 , wherein the biological sample is blood, plasma, or serum.
25. The method of claim 3 , wherein the control is a pre-established cut-off value.
26. The method of claim 3 , wherein the biological sample is blood, serum, plasma, urine, spinal fluid, saliva, lacrimal fluid, or sweat.
27. The method of claim 3 , wherein the biological sample is blood, plasma, or serum.
28. The method of claim 3 , wherein the concentration of the at least one protein is measured by an immunological method.
29. The method of claim 3 , wherein the concentration of the at least one protein is measured by mass spectrometry.
30. The method of claim 3 , wherein the JAK inhibitor is ruxolitinib.
31. The method of claim 3 , wherein the JAK inhibitor is itacitinib, 4-[3-(cyanomethyl)-3-(3′,5′-dimethyl-1H,l′H-4,4′-bipyrazol-1-yl) azetidin-1-yl]-2,5-difluoro-N-[(1S)-2,2,2-trifluoro-1-methylethyl] benzamide or a pharmaceutically acceptable salt thereof, or ((2R,5S)-5-{2- [(1R)-1-hydroxyethyl]-1H-imidazo [4,5-d]thieno [3,2-b]pyridin-1-yl}tetrahydro-2H-pyran-2-yl) acetonitrile or a pharmaceutically acceptable salt thereof.
32. The method of claim 3 , wherein the GvHD is acute GvHD.
33. The method of claim 3 , wherein the GvHD is chronic GvHD.
34. The method of claim 1 , comprising administering to the human subject a JAK inhibitor, wherein the human subject has been previously determined to have (i) a baseline concentration of IL8 protein in a biological sample obtained from the human subject that is lower than a control, wherein the control is the concentration of IL8 protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor, and/or (ii) a baseline concentration of NBL1 protein in a biological sample obtained from the human subject that is higher than a control, wherein the control is the concentration of NBL1 protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor.
35. The method of claim 34 , wherein the JAK inhibitor is ruxolitinib.
36. The method of claim 34 , wherein the JAK inhibitor is itacitinib, 4-[3-(cyanomethyl)-3-(3′,5′-dimethyl-1H,l′H -4,4′-bipyrazol-1-yl)azetidin-1-yl]-2,5-difluoro-N-[(1S) -2,2,2-trifluoro-1-methylethyl] benzamide or a pharmaceutically acceptable salt thereof, or ((2R,5S)-5-{2-[(1R)-1-hydroxyethyl]-1H-imidazo [4,5-d]thieno [3,2-b] pyridin-1-yl} tetrahydro-2H-pyran-2-yl)acetonitrile or a pharmaceutically acceptable salt thereof.
37. The method of claim 34 , wherein the biological sample is blood, plasma, or serum.
38. The method of claim 3 , comprising:
providing a biological sample obtained from the human subject;
measuring in the biological sample: (i) a reduced concentration, as compared to a control, of IL8 protein, wherein the control is the concentration of IL8 protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor, and/or (ii) an increased concentration, as compared to a control, of NBL1 protein, wherein the control is the concentration of NBL1 protein in a sample or samples obtained from one or more subjects having GvHD that have not responded to treatment with the JAK inhibitor; and
after the measuring, administering the JAK inhibitor to the human subject.
39. The method of claim 38 , wherein the JAK inhibitor is ruxolitinib.
40. The method of claim 38 , wherein the JAK inhibitor is itacitinib, 4-[3-(cyanomethyl)-3-(3′,5′-dimethyl-1H,1′H-4,4′-bipyrazol-1-yl) azetidin-1-yl]-2,5-difluoro-N-[(1S) -2,2,2-trifluoro-1-methylethyl]benzamide or a pharmaceutically acceptable salt thereof, or ((2R,5S)-5-{2-[(1R)-1-hydroxyethyl]-1H-imidazo [4,5-d]thieno [3,2-b]pyridin-1-yl}tetrahydro-2H-pyran-2-yl)acetonitrile or a pharmaceutically acceptable salt thereof.
41. The method of claim 38 , wherein the biological sample is blood, plasma, or serum.