Solid form of (-)-Ambrox formed by a bioconversion of homofarnesol in the presence of a biocatalyst
A solid form of (−)-Ambrox formed by a bioconversion process.
1. A solid form of the compound according to the formula (I)
wherein, said solid form:
exhibits an x-ray diffraction pattern having at least one of the following peaks at diffraction angles 2 theta of 15.6, 16.2, 16.7, 17.0, 17.4, 18.3+/−0.2°;
it comprises elongate crystals having an average diameter of at least 10 to 400 microns measured by laser granulometry;
it comprises elongate crystals having a length along their longest dimension of greater than 100 micron; and/or
it has an value of 90 or more; an value of less than 1 and greater the −1; and a value, which is less than 8, wherein , and values represent CIELAB chromaticity coordinates.
2. The solid form according to claim 1 , wherein the x-ray diffraction pattern exhibits all of the following peaks at diffraction angles 2 theta of 15.6, 16.2, 16.7, 17.0, 17.4 and 18.3+/−0.2°.
3. The solid form according to claim 2 , characterized by an x-ray diffraction pattern as depicted in FIG. 1 .
4. The solid form according to claim 1 , which is a product of a bioconversion process.
5. The solid form according to claim 4 , wherein the bioconversion process is an enzyme-catalyzed cyclization of homofarnesol comprising a mixture of 7E,3E and 7E,3Z geometric isomers of homofarnesol, wherein the reaction is carried out in the presence of a biocatalyst.
6. The solid form according to claim 5 , wherein the biocatalyst is a recombinant microorganism expressing the gene coding the enzyme, or an isolated enzyme, or an immobilized enzyme.
7. The solid form according to claim 6 , wherein the enzyme is a wild-type squalene hopene cyclase, or a variant of the wild-type squalene hopene cyclase.
8. The solid form according to claim 1 , having an value of 90 or more; an value of less than 1 and greater than −1; and a value, which is less than 8, wherein , and represent Cielab chromaticity coordinates.
9. A perfume composition comprising the solid form of (−)-Ambrox as defined in claim 1 , dissolved or dispersed in said composition.
10. A household care, personal care, laundry care or air care composition comprising the perfume composition according to claim 9 .
11. The solid form according to claim 1 , wherein it comprises elongate crystals having an average diameter of at least 10 to 400 microns measured by laser granulometry.
12. The solid form according to claim 1 , wherein it comprises elongate crystals having a length along their longest dimension of greater than 100 micron.
13. The solid form according to claim 6 , wherein the cyclization reaction is carried out in the presence of an SHC biocatalyst capable of bioconverting homofarnesol to (−)-Ambrox.