Combination therapies for treating myelodysplastic syndromes and acute myeloid leukemia
Methods, kits, and compositions are provided herein that can be used to treat hematopoietic disorders using an anti-CD47 agent such as an antibody and a hypomethylating agent, such as azacitidine.
1. A method of treating myelodysplastic syndrome (MDS) or acute myeloid leukemia (AML) in a subject comprising:
a. determining or having determined the presence of at least one p53 mutation in the subject, before administering step;
b. selecting the subject that has at least one p53 mutation; and
c. administering to the subject (i) magrolimab and (ii) azacitidine;
thereby treating the subject.
2. The method of claim 1 , wherein the subject is relapsed or refractory to at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or greater than 10 prior lines of cancer therapy.
3. The method of claim 1 , wherein the magrolimab is administered intravenously.
4. The method of claim 1 , wherein the azacitidine is administered intravenously, subcutaneously, or orally.
5. The method of claim 1 , wherein the administration of the magrolimab and the azacitidine reduces the p53 mutational burden in the subject relative to the p53 mutational burden present in the subject prior to the administration.
6. The method of claim 1 , wherein the administration of the magrolimab and the azacitidine reduces the level of leukemia stem cells present in the bone marrow of the subject as compared to the level of leukemia stem cells present in the bone marrow of the subject before the administration.
7. The method of claim 1 , wherein the method comprises administering the magrolimab and the azacitidine to the subject for at least two distinct cycles of four weeks each, the first cycle comprising (1) administering a priming dose of the magrolimab in the range of 1 mg to 10 mg of antibody per kg of body weight on Day 1 and 4, (2) administering a dose of at least 15 mg of the magrolimab per kg of body weight on day 8, (3) administering a dose of at least 30 mg of the magrolimab per kg of body weight on days 11, 15, and 22, and (4) administering a dose of at least 75 mg/m2 of the azacitidine on each of days 1-7; and the second cycle comprising (1) administering a dose of at least 30 mg of the magrolimab per kg of body weight once every week on days 1, 8, 15, and 22, and (2) administering a dose of at least 75 mg/m2 of the azacitidine on each of days 1-7.
8. The method of claim 7 , wherein the second cycle is repeated as one or more additional cycles without limit or until a clinical benefit is reduced or lost or no longer observed.
9. The method of claim 7 , wherein a third cycle of four weeks comprises (1) administering a dose of at least 30 mg of the magrolimab per kg of body weight once every two weeks on days 1 and 15, and (2) administering a dose of at least 75 mg/m2 of the azacitidine on each of days 1-7.
10. The method of claim 7 , wherein a third cycle of four weeks comprises (1) administering a dose of at least 30 mg of the magrolimab per kg of body weight once every week on days 1, 8, 15, and 22 and (2) administering a dose of at least 75 mg/m2 of the azacitidine on each of days 1-7.
11. The method of claim 9 , wherein the third cycle is repeated as one or more additional cycles without limit or until a clinical benefit is reduced or lost or no longer observed.
12. The method of claim 1 , wherein the subject is a human subject.