Stable inoculant compositions and methods for producing same
The present disclosure provides non-aqueous inoculant compositions and methods for enhancing the survival and/or stability of microbial spores in an inoculant composition. In some embodiments, inoculant compositions of the present disclosure comprise microbial spores, one or more dispersants, one or more dust suppressants and a solid non-aqueous carrier.
1. An inoculant composition comprising fungal spores in a carrier,
said carrier comprising one or more maltodextrins having a dextrose equivalent value of about 15 to about 20 and one or more disaccharides, and
said one or more maltodextrins and said one or more disaccharides comprising about 70 to about 95% w/w (weight by weight) of said inoculant composition based upon the total weight of said inoculant composition.
2. The inoculant composition of claim 1 , in which said fungal spores comprise about 5 to about 30% w/w of said inoculant composition based upon the total weight of said inoculant composition.
3. The inoculant composition of claim 1 , in which said fungal spores comprise about 5 to about 15% w/w of said inoculant composition based upon the total weight of said inoculant composition.
4. The inoculant composition of claim 1 , in which said fungal spores are present in a concentration ranging from about 1×10 1 to about 1×10 15 colony-forming units per gram of said inoculant composition.
5. The inoculant composition of claim 1 , in which said fungal spores are present in a concentration ranging from about 1×10 4 to about 1×10 11 colony-forming units per gram of said inoculant composition.
6. The inoculant composition of claim 1 , in which said fungal spores are present at a concentration of at least 1×10 7 colony-forming units per gram of said inoculant composition.
7. The inoculant composition of claim 1 , in which said one or more disaccharides comprises maltose.
8. The inoculant composition of claim 1 , in which said one or more maltodextrins and said one or more disaccharides are present in a maltodextrin:disaccharide ratio of about 75:25 to about 95:5.
9. The inoculant composition of claim 1 , in which said one or more maltodextrins and said one or more disaccharides comprise about 85 to about 95% w/w of said inoculant composition based upon the total weight of said inoculant composition.
10. The inoculant composition of claim 1 , in which said carrier further comprises one or more dispersants.
11. The inoculant composition of claim 10 , in which said one or more dispersants comprise about 2.5 to about 7.5% w/w of said inoculant composition based upon the total weight of said inoculant composition.
12. An inoculant composition comprising fungal spores in a carrier,
said carrier comprising one or more maltodextrins and one or more disaccharides in a maltodextrin:disaccharide ratio of about 85:15 to about 95:5, and
said one or more maltodextrins and said one or more disaccharides comprising about 70 to about 95% w/w (weight by weight) of said inoculant composition based upon the total weight of said inoculant composition.
13. The inoculant composition of claim 12 , in which said one or more maltodextrins and said one or more disaccharides are present in a maltodextrin:disaccharide ratio of about 90:10.
14. An inoculant composition comprising fungal spores in a carrier,
said carrier comprising one or more maltodextrins having a dextrose equivalent value of about 15 to about 20 and maltose,
said one or more maltodextrins and said maltose being present in a maltodextrin:maltose ratio of about 75:25 to about 95:5, and
said one or more maltodextrins and said maltose comprising about 70 to about 95% w/w (weight by weight) of said inoculant composition based upon the total weight of said inoculant composition.
15. The inoculant composition of claim 14 , in which said one or more maltodextrins and said maltose are present in a maltodextrin:maltose ratio of about 85:15 to about 95:5.
16. The inoculant composition of claim 14 , in which said one or more maltodextrins and said maltose are present in a maltodextrin:maltose ratio of about 90:10.
17. The inoculant composition of claim 14 , in which said one or more maltodextrins and said maltose comprise about 85 to about 95% w/w of said inoculant composition based upon the total weight of said inoculant composition.