Multiplexed amplitude modulation photometer and performing multiplexed amplitude modulation photometry
A multiplexed amplitude modulation photometer includes a microchannel; a first input light path that: receives a first modulated light at a first modulation frequency; and communicates the first modulated light to a first optical region that receives the first analyte that produces a first output light including the first modulation frequency communicated to a first detection light path; the first optical region; the first detection light path that receives the first output light; a second input light path that: receives a second modulated light with a second modulation frequency; and communicates second modulated light to a second optical region that receives the second analyte that produces a second output light with the second modulation frequency communicated to a second detection light path; the second optical region; and the second detection light path that receives the second output light from the second optical region.
1. A process for performing multiplexed amplitude modulation photometry, the process comprising:
introducing a first analyte in a first optical region;
subjecting the first analyte to a first modulated light, the first modulated light comprising a first wavelength that is modulated at a first modulation frequency;
producing, from the first analyte, first output light in response to subjecting the first analyte to the first modulated light, the first output light modulated at the first modulation frequency;
propagating the first output light to an optical detector from the first optical region;
receiving, by the optical detector, the first output light;
introducing a second analyte in a second optical region, the second optical region separated from the first optical region;
subjecting the second analyte to a second modulated light, the second modulated light comprising a second wavelength that is modulated at a second modulation frequency, the second modulation frequency being different than the first modulation frequency;
producing, from the second analyte, second output light in response to subjecting the second analyte to the second modulated light, the second output light modulated at the second modulation frequency;
propagating the second output light to the optical detector from the second optical region;
receiving, by the optical detector, the second output light;
producing, by the optical detector, a multiplexed amplitude modulation signal from the first output light and the second output light;
determining, from the multiplexed amplitude modulation signal, a first amount c1 of the first analyte in the first optical region subjected to the first modulated light and a second amount c2 of the second analyte in the second optical region subjected to the second modulated light to perform multiplexed amplitude modulation photometry;
receiving, by a demodulator, the multiplexed amplitude modulation signal from the optical detector;
deconvolving, by the demodulator, the multiplexed amplitude modulation signal to produce a first demodulated signal and a second demodulated signal;
subjecting first demodulated signal to a first frequency filter to obtain the first amount c1 of first analyte; and
subjecting second demodulated signal to a second frequency filter to obtain the second amount c2 of second analyte.
2. The process of claim 1 , wherein deconvolving the multiplexed amplitude modulation signal comprises applying sin(w1*t) to the multiplexed amplitude modulation signal as:
[ c 1( t )*( a 1+ b 1*sin( w 1* t ))+ c 2( t )*( a 2+ b 2*sin( w 2* t ))]*sin( w 1* t )
to produce the first demodulated signal.
3. The process of claim 1 , wherein deconvolving the multiplexed amplitude modulation signal comprises applying sin(w2*t) to the multiplexed amplitude modulation signal as:
[ c 1( t )*( a 1+ b 1*sin( w 1* t ))+ c 2( t )*( a 2+ b 2*sin( w 2* t ))]*sin( w 2* t )
to produce the second demodulated signal.
4. The process of claim 1 , further comprising receiving, by the demodulator, the first modulation frequency of the first output light.
5. The process of claim 1 , further comprising receiving, by the demodulator, the second modulation frequency of the second output light.
6. The process of claim 1 , further comprising, prior to producing the multiplexed amplitude modulation signal by the optical detector:
receiving, by a beam combiner, the first output light and the second output light;
optically combining, by the beam combiner, the first output light and the second output light;
communicating the combined first output light and second output light from the beam combiner to the optical detector; and
receiving, by the demodulator, the combined first output light and second output light from the beam combiner.