Control of evaporation in digital microfluidics
Air-matrix digital microfluidics (DMF) apparatuses and methods of using them. These methods and apparatuses may include the use of a liquid wax coating material and/or pinning the encapsulated reaction droplet within the air gap using pinning features. Any of these methods may also include separating the liquid wax from an encapsulated aqueous droplet, e.g., using an oil absorbent wick to selectively separate the liquid oil or wax from the aqueous droplet by adsorbing and/or absorbing the liquid wax into the absorbent wick while leaving the aqueous droplet behind.
1. A digital microfluidics method, the method comprising:
driving a droplet within an air gap of an air-matrix digital microfluidic (DMF) apparatus to a sub-region of the air gap by electrowetting;
coating the droplet with a liquid wax material within the air gap;
pinning the droplet within the sub-region by contacting the droplet with two or more protrusions extending from an upper surface of the air gap into the air gap, wherein the two or more protrusions extend only partially into the air gap;
vortexing a plurality of magnetic beads within the coated droplet within a thermal zone; and
performing one or more manipulations on the pinned droplet.
2. The method of claim 1 , wherein pinning the droplet comprises pinning at least the liquid wax coating material coating the droplet.
3. The method of claim 1 , wherein performing one or more manipulations comprises heating the sub-region of the air gap including the droplet.
4. The method of claim 1 , further comprising driving the droplet away from the sub-region and off of the protrusions by electrowetting.
5. The method of claim 1 , wherein the two or more protrusions comprise three to ten protrusions.
6. The method of claim 1 , wherein each of the two or more protrusions have a cylindrical or rectangular shape.
7. The method of claim 1 , wherein each of the two or more protrusions have a lateral dimension on the upper surface of the air gap between 0.5 mm to 2.8 mm.
8. The method of claim 1 , wherein each of the two or more protrusions have a vertical dimension extending into the air gap of 1% to 90% of a vertical dimension of the air gap.
9. The method of claim 1 , wherein each of the two or more protrusions have a vertical dimension extending into the air gap of 1% to 20% of a vertical dimension of the air gap.
10. The method of claim 1 , wherein performing one or more manipulations comprises performing at least one of: vortexing a plurality of magnetic beads within the pinned droplet, cooling the sub-region of the air gap including the pinned droplet, detecting the pinned droplet; driving the pinned droplet to a channel hole within the sub-region of the air gap; aspirating the droplet into a channel of the channel hole; and driving the droplet from the sub-region by electrowetting.
11. A digital microfluidic (DMF) method comprising:
coating an aqueous reaction droplet with a liquid wax material within an air gap formed between a first hydrophobic layer of a first plate and a second hydrophobic layer of a second plate of a DMF apparatus;
pinning the liquid wax coating of the aqueous reaction droplet to at least two protrusions within a sub-region of the air gap, thereby distributing the liquid wax around the reaction droplet;
heating at least the sub-region of the air gap including the coated aqueous reaction droplet, whereby the liquid wax coating limits or prevents evaporation from the aqueous reaction droplet; and
vortexing a plurality of magnetic beads within the coated reaction droplet within a thermal zone.
12. The method of claim 11 , wherein pinning comprises pinning the liquid wax coating to the at least two protrusions disposed adjacent to the second hydrophobic layer of the second plate.
13. The method of claim 11 , wherein the at least two protrusions comprise two to ten protrusions.
14. The method of claim 11 , wherein each of the at least two protrusions has a cylindrical or rectangular shape.
15. The method of claim 11 , wherein each of the at least two protrusions has a lateral dimension between 0.5 mm to 2.8 mm.
16. The method of claim 11 , wherein each of the at least two protrusions has a vertical dimension extending into the air gap of 1% to 90% of a vertical dimension of the air gap.
17. The method of claim 11 , wherein each of the at least two protrusions has a vertical dimension into the air gap extending less than 40% of a vertical dimension of the air gap.
18. The method of claim 11 , wherein each of the at least two protrusions has a vertical dimension extending into the air gap between 1% to 20% of a vertical dimension of the air gap.
19. The method of claim 11 , further comprising moving the coated aqueous reaction droplet away from the sub-region of the air gap after completing heating.
20. The method of claim 19 , wherein moving comprises driving the coated aqueous reaction droplet by energizing a sub-set of a plurality of driving electrodes adjacent to the first hydrophobic layer of the first plate of the DMF apparatus.
21. The method of claim 19 , wherein moving further comprises withdrawing at least a portion of the coated aqueous reaction droplet from a surface of the air gap of the DMF apparatus before energizing a sub-set of the plurality of driving electrodes; and
reintroducing the at least portion of the coated aqueous reaction droplet back to the surface of the air gap of the DMF apparatus as a front of the aqueous reaction droplet exits the sub-region.
22. The method of claim 21 , wherein withdrawing comprises withdrawing the at least portion of the coated reaction droplet via a channel from a surface of the second hydrophobic layer of the second plate to at least partially through the second plate.
23. The method of claim 11 , wherein the first plate comprises a bottom plate of a cartridge configured to be seated on a seating surface of the DMF apparatus comprising a plurality of actuation electrodes.
24. A digital microfluidic (DMF) method comprising:
coating an aqueous reaction droplet with a liquid wax material within an air gap formed between a first hydrophobic layer of a first plate and a second hydrophobic layer of a second plate of a DMF apparatus, wherein the first plate comprises a bottom plate of a cartridge configured to be seated on a seating surface of the DMF apparatus comprising a plurality of actuation electrodes;
pinning the liquid wax coating of the aqueous reaction droplet to at least two protrusions within a sub-region of the air gap, thereby distributing the liquid wax around the reaction droplet; and
heating at least the sub-region of the air gap including the coated aqueous reaction droplet, whereby the liquid wax coating limits or prevents evaporation from the aqueous reaction droplet.