AGGREGATED CELL MASS DISPERSING DEVICE AND METHOD OF DISPERSING AGGREGATED CELL MASS
An aggregated cell mass dispersing device ( 100 ) of the present invention includes: a storage container ( 101 ) storing a liquid (L) containing an aggregated cell mass; a pipette ( 102 ) whose tip is pushed against an inner bottom surface ( 101 a ) of the storage container; and an elastic body ( 103 ) supporting an outer bottom surface of the storage container.
1 . An aggregated cell mass dispersing device, comprising:
a storage container storing a liquid comprising an aggregated cell mass;
a pipette whose tip is pushed against an inner bottom surface of the storage container; and
an elastic body supporting an outer bottom surface of the storage container.
2 . The aggregated cell mass dispersing device of claim 1 ,
wherein the elastic body is a spring disposed to extend and contract in a direction parallel to a direction in which the pipette is pushed, and
one end of the spring is fixed to the outer bottom surface of the storage container.
3 . The aggregated cell mass dispersing device of claim 1 ,
wherein the elastic body is provided with a first support member supporting the storage container at one side and a spring disposed to extend and contract in a direction parallel to a direction in which the pipette is pushed, and
one end of the spring is fixed to the other side of the first support member.
4 . The aggregated cell mass dispersing device of claim 1 , wherein the elastic body is provided with a second support member to which an end of a spring is fixed and which supports the spring.
5 . The aggregated cell mass dispersing device of claim 1 , wherein the pipette becomes thinner toward the tip.
6 . The aggregated cell mass dispersing device of claim 1 , wherein a section on a bottom of the storage container, against which the tip of the pipette is pushed, is recessed toward the elastic body side.
7 . The aggregated cell mass dispersing device of claim 1 , wherein the storage container is one or more tubes whose bottom side is closed, and which is provided upright so as to extend opposite to the bottom.
8 . The aggregated cell mass dispersing device of claim 7 , further comprising a guide member surrounding an outer peripheral portion of the one or more tubes.
9 . A method of dispersing an aggregated cell mass using the aggregated cell mass dispersing device of claim 1 , the method comprising:
storing a liquid comprising an aggregated cell mass in the storage container;
pushing the tip of the pipette against the inner bottom surface of the storage container; and
pulling the tip of the pipette away from the inner bottom surface of the storage container.
10 . The method of claim 9 , wherein the pushing and the pulling are repeated 50 times or more alternately.