Prime/boost immunization regimen against HIV-1 utilizing a multiepitope T cell immunogen comprising Gag, Pol, Vif, and Nef epitopes
The present invention relates to immunogenic therapies for the treatment or prevention of a human immunodeficiency virus (HIV) infection or a disease associated with an HIV infection.
1. A method for inducing an immune response against a human immunodeficiency virus (HIV) in a subject in need thereof comprising a prime-boost regimen of:
(a) administering to the subject 1 to 10 administrations of an immunogenically effective amount of a first viral vector encoding an immunogenic polypeptide as a prime, followed by;
(b) administering to the subject 1 to 10 administrations of an immunogenically effective amount of a second viral vector encoding the immunogenic polypeptide as a boost;
wherein said immunogenic polypeptide comprises:
(i) a sequence having at least 95% identity to the sequence of SEQ ID NO:1,
(ii) a sequence having at least 95% identity to the sequence of SEQ ID NO:2,
(iii) a sequence having at least 95% identity to the sequence of SEQ ID NO:3,
(iv) a sequence having at least 95% identity to the sequence of SEQ ID NO:4,
(v) a sequence having at least 95% identity to the sequence of SEQ ID NO:5,
(vi) a sequence having at least 95% identity to the sequence of SEQ ID NO:6,
(vii) a sequence having at least 95% identity to the sequence of SEQ ID NO:7,
(viii) a sequence having at least 95% identity to the sequence of SEQ ID NO:8,
(ix) a sequence having at least 95% identity to the sequence of SEQ ID NO:9,
(x) a sequence having at least 95% identity to the sequence of SEQ ID NO:10,
(xi) a sequence having at least 95% identity to the sequence of SEQ ID NO:11,
(xii) a sequence having at least 95% identity to the sequence of SEQ ID NO:12,
(xiii) a sequence having at least 95% identity to the sequence of SEQ ID NO:13,
(xiv) a sequence having at least 95% identity to the sequence of SEQ ID NO:14,
(xv) a sequence having at least 95% identity to the sequence of SEQ ID NO:15, and
(xvi) a sequence having at least 95% identity to the sequence of SEQ ID NO:16.
2. A method of treating or preventing a human immunodeficiency virus (HIV) infection or a disease associated with an HIV infection in a subject in need thereof, comprising a prime-boost regimen of:
(a) administering to the subject 1 to 10 administrations of a first viral vector encoding an immunogenic polypeptide as a prime, followed by;
(b) administering to the subject 1 to 10 administrations of a second viral vector encoding the immunogenic polypeptide as a boost;
wherein the immunogenic polypeptide comprises:
(i) a sequence having at least 95% identity to the sequence of SEQ ID NO:1,
(ii) a sequence having at least 95% identity to the sequence of SEQ ID NO:2,
(iii) a sequence having at least 95% identity to the sequence of SEQ ID NO:3,
(iv) a sequence having at least 95% identity to the sequence of SEQ ID NO:4,
(v) a sequence having at least 95% identity to the sequence of SEQ ID NO:5,
(vi) a sequence having at least 95% identity to the sequence of SEQ ID NO:6,
(vii) a sequence having at least 95% identity to the sequence of SEQ ID NO:7,
(viii) a sequence having at least 95% identity to the sequence of SEQ ID NO:8,
(ix) a sequence having at least 95% identity to the sequence of SEQ ID NO:9,
(x) a sequence having at least 95% identity to the sequence of SEQ ID NO:10,
(xi) a sequence having at least 95% identity to the sequence of SEQ ID NO:11,
(xii) a sequence having at least 95% identity to the sequence of SEQ ID NO:12,
(xiii) a sequence having at least 95% identity to the sequence of SEQ ID NO:13,
(xiv) a sequence having at least 95% identity to the sequence of SEQ ID NO:14,
(xv) a sequence having at least 95% identity to the sequence of SEQ ID NO:15, and
(xvi) a sequence having at least 95% identity to the sequence of SEQ ID NO:16.
3. The method of claim 1 , wherein at least two of the sequences (i)-(xvi) are joined by a single, dual, or triple alanine amino acid linker, wherein the linker results in the formation of an AAA sequence in the junction region between adjoining sequences.
4. The method of claim 1 , wherein the sequence of each of (i) to (xvi) is 11-85 amino acids in length.
5. The method of claim 1 , wherein (a) comprises administering to the subject 1 to 4 administrations of the first viral vector encoding the immunogenic polypeptide, and/or (b) comprises administering to the subject 1 to 4 administrations of the second viral vector encoding the immunogenic polypeptide.
6. The method of claim 1 , wherein (a) comprises administering to the subject one administration of the first viral vector encoding the immunogenic polypeptide, and (b) comprises administering to the subject one administration of the second viral vector encoding the immunogenic polypeptide.
7. The method of claim 1 , wherein the first viral vector is chimpanzee adenovirus (ChAd) vector and the second viral vector is a Modified Vaccinia Ankara (MVA) vector.
8. The method of claim 7 , wherein the chimpanzee adenovirus vector is ChAdOx1.HTI and the Modified Vaccinia Ankara (MVA) virus vector is MVA.HTI.
9. A method for inducing an immune response against a human immunodeficiency virus (HIV) in a subject in need thereof, the method consisting essentially of a prime-boost regimen of:
(a) administering to the subject 1 to 5 administrations of a first viral vector encoding the immunogenic polypeptide as a prime, followed by;
(b) administering to the subject 1 to 5 administrations of a second viral vector encoding the immunogenic polypeptide as a boost;
wherein the immunogenic polypeptide comprises:
(i) a sequence having at least 95% identity to the sequence of SEQ ID NO:1,
(ii) a sequence having at least 95% identity to the sequence of SEQ ID NO:2,
(iii) a sequence having at least 95% identity to the sequence of SEQ ID NO:3,
(iv) a sequence having at least 95% identity to the sequence of SEQ ID NO:4,
(v) a sequence having at least 95% identity to the sequence of SEQ ID NO:5,
(vi) a sequence having at least 95% identity to the sequence of SEQ ID NO:6,
(vii) a sequence having at least 95% identity to the sequence of SEQ ID NO:7,
(viii) a sequence having at least 95% identity to the sequence of SEQ ID NO:8,
(ix) a sequence having at least 95% identity to the sequence of SEQ ID NO:9,
(x) a sequence having at least 95% identity to the sequence of SEQ ID NO:10,
(xi) a sequence having at least 95% identity to the sequence of SEQ ID NO:11,
(xii) a sequence having at least 95% identity to the sequence of SEQ ID NO:12,
(xiii) a sequence having at least 95% identity to the sequence of SEQ ID NO:13,
(xiv) a sequence having at least 95% identity to the sequence of SEQ ID NO:14,
(xv) a sequence having at least 95% identity to the sequence of SEQ ID NO:15, and
(xvi) a sequence having at least 95% identity to the sequence of SEQ ID NO:16.
10. The method of claim 9 , wherein (a) comprises administering to the subject a single administration of the first viral vector encoding the immunogenic and/or (b) comprises administering to the subject a single administration of the second viral vector encoding the immunogenic polypeptide.
11. The method of claim 9 , wherein the first viral vector is a ChAd vector and/or the second viral vector is an MVA vector.
12. The method of claim 1 , wherein the first viral vector is administered at a dose of from about 1×10 8 to about 1×10 11 viral particles (vp).
13. The method of claim 12 , wherein the first vector is administered at a dose of about 5×10 10 vp.
14. The method of claim 1 , wherein the second viral vector is administered at a dose of about 1×10 6 to about 1×10 10 plaque forming units (pfu).
15. The method of claim 14 , wherein the second viral vector is administered at a dose of about 2×10 8 pfu.
16. The method of claim 1 , wherein the administration of the first viral vector and the administration of the second viral vector is separated by a period of about two weeks to about 24 weeks.
17. The method of claim 16 , wherein the period between the administration of the first viral vector and the administration of the second viral vector is about 8 weeks.
18. The method of claim 1 , wherein the immunogenic polypeptide further comprises a signal peptide at the N-terminus of the immunogenic polypeptide.
19. The method of claim 1 , wherein the immunogenic polypeptide comprises the sequence of SEQ ID NO:99 or wherein the immunogenic polypeptide is encoded by a nucleic acid comprising SEQ ID NO:100 or 101.
20. The method of claim 2 , wherein the disease associated with an HIV infection is an acquired immune deficiency syndrome (AIDS), AIDS-related complex (ARC), or HIV opportunistic disease.
21. The method of claim 20 , wherein the HIV is HIV type 1 (HIV-1) or HIV type 2 (HIV-2).
22. The method of claim 1 , wherein the subject is a human subject.
23. The method of claim 1 , wherein the first viral vector is administered in a pharmaceutical composition comprising L-Histidine: 10 mM; NaCl: 35 mM; Sucrose: 7.5% (w/v); MgCl 2 : 1 mM; EDTA disodium: 0.1 mM; Tween 80 (Polysorbate-80): 0.1% (w/v); Ethanol: 0.5% (v/v); HCl: Adjusted to pH 6.6, and/or the second viral vector is administered in a pharmaceutical composition comprising 0.5 mL Tris buffer (10 mM Tris HCl, pH 7.7, 140 mM NaCl).
24. The method of claim 2 , wherein the method of treating or preventing a HIV infection comprises achieving virologic suppression in the subject.
25. The method of claim 2 , wherein the method of treating or preventing a HIV infection comprises maintaining virologic suppression in the subject.
26. The method of claim 1 , wherein the method further comprises administering to the subject one or more other anti-HIV therapies in conjunction with the first and second viral vectors encoding the immunogenic polypeptide.
27. The method according to claim 26 , wherein said one or more anti-HIV therapies are administered sequentially or concurrently with the first and second viral vectors encoding the immunogenic polypeptide.
28. The method of claim 1 , wherein the method treats or prevents a human immunodeficiency virus (HIV) infection or a disease associated with an HIV infection in the subject.
29. The method of claim 28 , wherein the disease associated with an HIV infection is an acquired immune deficiency syndrome (AIDS), AIDS-related complex (ARC), or HIV opportunistic disease.
30. The method of claim 29 , wherein the HIV is HIV type 1 (HIV-1) or HIV type 2 (HIV-2).
31. The method of claim 28 , wherein the method comprises achieving virologic suppression in the subject.
32. The method of claim 28 , wherein the method of treating or preventing a HIV infection comprises maintaining virologic suppression in the subject.