Proline hydroxylase and uses thereof
Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.
1. A proline hydroxylase, comprising a protein having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids, wherein the mutation of one or more amino acids must comprise E27K, and the mutation of one or more amino acids is selected from the group consisting of: H14R, L16N, T25R, F26L, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A and having proline hydroxylase activity.
2. The proline hydroxylase of claim 1 , wherein the mutation comprises any one of the combinations selected from the group consisting of: E27K+Y35W/K, E27K+K123/D/I/Q/S, E27K+N165H, E27K+S37C/E/F/N/W/T+I223V K123D/I/Q/S, I223V+S37C/E/F/N/W/T+E27K+N165H, wherein ‘/’ represents ‘or’.
3. A method for producing an L-hydroxyproline derivative, wherein the method comprises:
contacting an L-proline derivative substrate with the proline hydroxylase of claim 1 to catalyze hydroxylation of the L-proline derivative substrate, to obtain the L-hydroxyproline derivative as shown in general formula (I):
wherein R 1 is selected from C 1 -C 5 alkylene or C 2 -C 5 alkenylene; R 2 is selected from C 0 -C 4 alkylene or C 2 -C 4 alkenylene; R 3 is selected from hydroxyl, amino, C 1 -C 6 alkoxy, aryloxy, C 1 -C 6 alkyl sulfenyl or C 1 -C 6 aryl sulfenyl; and R 4 is selected from hydrogen, C 1 -C 6 alkyl, C 2 -C 6 alkenyl or C 2 -C 6 alkynyl.
4. The method of claim 3 , wherein the method further comprises: adding -oxoglutarate and O 2 as a common substrate and adding iron ions as a cofactor.
5. The method of claim 3 , wherein the L-hydroxyproline derivative is cis-4-hydroxy-L-proline or (2S,5S)-5-hydroxypiperidine-2-carboxylic acid.
6. The method of claim 3 , wherein the proline hydroxylase catalyzes hydroxylation of the L-proline derivative substrate in a temperature of 5-45° C. to obtain the L-hydroxyproline derivative as shown in the general formula (I).
7. The method of claim 6 , wherein the proline hydroxylase catalyzes hydroxylation of the L-proline derivative substrate in a temperature of 5-15° C. to obtain the L-hydroxyproline derivative as shown in the general formula (I).