CAMELIDAE SINGLE-DOMAIN ANTIBODIES AGAINST YERSINIA PESTIS AND METHODS OF USE
Single-domain antibodies (SAbs) against three Yersinia pestis surface proteins (LcrV, YscF, and F1), nucleic acid sequences encoding the SAbs, and polypeptides comprising two or more SAbs capable of recognizing two or more epitopes and/or antigens. The present invention further includes methods for preventing or treating Y. pestis infections in a patient; methods for detecting and/or diagnosing Y. pestis infections; and devices and methods for identifying and/or detecting Y. pestis on a surface and/or in an environment.
1 . A single-domain antibody against Yersinia pestis ( Y. pestis ) LcrV protein comprising:
a first framing region (“FR”) sequence comprising SEQ ID No:96 or SEQ ID No:99;
a first complementarity determining region (“CDR”) sequence comprising SEQ ID No:22;
a second FR sequence comprising SEQ ID No:116, the first CDR sequence being positioned between the first FR sequence and the second FR sequence;
a second CDR sequence comprising SEQ ID No:49;
a third FR sequence comprising SEQ ID No: 141, the second CDR sequence being positioned between the second FR sequence and the third FR sequence;
a third CDR sequence comprising SEQ ID No.73 or SEQ ID No.75; and
a fourth FR sequence comprising SEQ ID No:148, the third CDR sequence being positioned between the third FR sequence and the fourth FR sequence.
2 . The single-domain antibody of claim 1 , wherein the at least one single-domain antibody further comprises:
at least one of a protein tag, a protein domain tag, or a chemical tag.
3 . The single-domain antibody of claim 1 , further comprising:
a plurality of single-domain antibodies, wherein the single-domain antibodies of the plurality are against the Y. pestis LcrV protein and a Y. pestis YscF protein or a Y. pestis F1 protein.
4 . A polypeptide comprising:
a plurality of the single-domain antibodies of claim 1 organized into a chain.
5 . The polypeptide of claim 4 , wherein at least a portion of the plurality of single-domain antibodies comprising the polypeptide is against a different epitope on the LcrV protein.
6 . The polypeptide of claim 4 , wherein the plurality of single-domain antibodies comprising the polypeptide is against the Y. pestis LcrV protein and a Y. pestis YscF protein or a Y. pestis F1 protein.
7 . The polypeptide of claim 4 , further comprising:
a fusion protein.
8 . The polypeptide of claim 4 , further comprising:
a multivalent protein complex such that the single-domain antibodies of the plurality are joined together with at least one linker molecule.
9 . The polypeptide of claim 4 , wherein at least one of the plurality of single-domain antibodies comprising the polypeptide further comprises:
at least one of a protein tag, a protein domain tag, or a chemical tag.
10 . At least one isolated nucleotide sequence encoding the at least one single-domain antibody of claim 1 , wherein the isolated nucleotide sequence is selected from the group consisting of SEQ ID No:212 or SEQ ID No:213.