Compositions and methods for cell-like calibration particles
A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.
1. A method, comprising:
calibrating a cytometric device for analysis of a target cell, by:
providing a hydrogel particle having a background fluorescent property and a spectral property that are substantially similar to the background fluorescent property and the spectral property of the target cell, respectively;
binding a reagent to the hydrogel particle to form a complex;
inserting the complex into the cytometric device;
measuring at least one property of the complex using the cytometric device; and
calculating a spectral unmixing based on the at least one measured property.
2. The method of claim 1 , wherein the reagent includes a fluorophore.
3. The method of claim 1 , wherein the hydrogel particle has been modified to bind to an antibody that is bound to a conjugated fluorophore.
4. The method of claim 3 , wherein the conjugated fluorophore is a fluorochrome.
5. The method of claim 1 , wherein the hydrogel particle is a modified hydrogel particle that has been modified to bind to at least one of an intercalating nucleic acid labeling reagent or an amine-reactive nucleic acid labeling reagent.
6. The method of claim 5 , further comprising using the modified hydrogel particle to assess a viability of the target cell.
7. The method of claim 1 , wherein the hydrogel particle has a refractive index of greater than about 1.15.
8. The method of claim 1 , wherein the hydrogel particle has a refractive index of greater than about 1.3.
9. The method of claim 1 , wherein the hydrogel particle has a refractive index of greater than about 1.7.
10. The method of claim 1 , wherein the hydrogel particle has a diameter of less than about 100 μm.
11. The method of claim 1 , wherein the hydrogel particle has a diameter of less than about 10 μm.
12. The method of claim 1 , wherein the hydrogel particle has a diameter of less than about 1 μm.
13. The method of claim 1 , wherein the hydrogel particle contains polymer nanoparticle additives.
14. The method of claim 1 , wherein the hydrogel particle is a chemically functionalized hydrogel particle.
15. The method of claim 1 , wherein the hydrogel particle comprises a free amine group.
16. The method of claim 1 , wherein the hydrogel particle comprises allylamine.
17. The method of claim 1 , wherein the target cell is an immune cell.
18. The method of claim 1 , further comprising polymerizing a droplet to produce the hydrogel particle.
19. The method of claim 1 , wherein the hydrogel particle is a hydrogel particle that has been modified by conjugating or attaching one of a fluorophore or a fluorochrome.
20. The method of claim 19 , wherein the modified hydrogel particle matches a fluorescence profile of a cell.
21. The method of claim 1 , wherein the at least one property includes one of inter-laser delay, fluorescence response, or sort timing.
22. The method of claim 1 , further comprising generating a spectral unmixing table based on the calculated spectral unmixing.
23. A method, comprising:
calculating a plurality of adjustment values for a cytometric measurement of a target cell, by:
inserting, into the cytometric device, a first hydrogel particle and a second hydrogel particle, the first hydrogel particle having a background fluorescent property and a spectral property that are substantially similar to the background fluorescent property and the spectral property of the target cell, respectively, and the second hydrogel particle one of configured to bind to a reagent or pre-bound to the reagent, the reagent being a reagent that generates a fluorescent signal different from the background fluorescent property,
measuring at least one property of the first hydrogel particle and at least one property of the second hydrogel particle using the cytometric device, and
calculating a spectral unmixing based on the at least one measured property of the second hydrogel particle; and
modifying the cytometric measurement of the target cell based on the plurality of adjustment values.
24. A method, comprising:
calibrating a cytometric device for analysis of a target cell, by:
providing a hydrogel particle having at least one of a background fluorescent property or a spectral profile that is substantially similar to the at least one of a background fluorescent property or a spectral profile of the target cell, respectively;
binding a reagent to the hydrogel particle to form a complex;
inserting the complex into the cytometric device;
measuring at least one property of the complex using the cytometric device; and
calculating a spectral unmixing based on the at least one measured property.
25. The method of claim 24 , wherein the reagent includes a fluorophore.
26. The method of claim 24 , wherein the hydrogel particle has been modified to bind to an antibody that is bound to a conjugated fluorophore.
27. The method of claim 24 , wherein the hydrogel particle is a modified hydrogel particle that has been modified to bind to at least one of an intercalating nucleic acid labeling reagent or an amine-reactive nucleic acid labeling reagent.
28. The method of claim 24 , wherein the hydrogel particle has a diameter of less than about 100 μm.
29. The method of claim 24 , wherein the hydrogel particle contains polymer nanoparticle additives.
30. The method of claim 24 , wherein the hydrogel particle is a chemically functionalized hydrogel particle.
31. The method of claim 24 , further comprising polymerizing a droplet to produce the hydrogel particle.