Tissue specific reduction of lignin
View Patent ↗The present invention provides an expression cassette comprising a polynucleotide that encodes a protein that diverts a monolignol precursor from a lignin biosynthesis pathway in the plant, which is operably linked to a heterologous promoter. Also provided are methods of engineering a plant having reduced lignin content, as well as plant cells, plant parts, and plant tissues from such engineered plants.
1. A method of engineering a plant having reduced lignin content, the method comprising:
introducing into the plant an expression cassette comprising a polynucleotide that encodes a protein that reduces the amount of coumaroyl-CoA, caffeoyl-CoA, and/or feruloyl-CoA that is available for a lignin biosynthesis pathway in the plant, wherein the protein is a 2-oxoglutarate-dependent dioxygenase (C2′H) having at least 95% amino acid sequence identity to SEQ ID NO:14; or the protein is a curcuminoid synthase having at least 95% amino acid sequence identity to SEQ ID NO:34 or SEQ ID NO:35; and further, wherein the polynucleotide is operably linked to a heterologous secondary cell wall-specific promoter, a fiber cell-specific promoter, or a promoter from a gene in the lignin biosynthesis pathway; and
culturing the plant under conditions in which the protein is expressed.
2. The method of claim 1 , wherein the protein is the 2-oxoglutarate-dependent dioxygenase (C2′H) and comprises the amino acid sequence of SEQ ID NO:14.
3. The method of claim 2 , wherein the protein is the curcuminoid synthase and comprises the amino acid sequence of SEQ ID NO:34 or SEQ ID NO:35.
4. A plant engineered by the method of claim 1 .
5. A plant cell from the plant of claim 4 , wherein plant cell comprises the expression cassette.
6. A seed, flower, leaf, or fruit from the plant of claim 4 , wherein the seed, flower, leaf, or fruit comprises the expression cassette.