Apparatus for analysis of eggs
The present invention relates to a process for the non-destructive determination of gender, developmental stage and/or viability of an avian embryo in an egg, comprising (a) detecting at least a first developmental marker compound selected from sugars and/or amino acids, precursors and metabolites thereof in an egg at a time period of from the beginning of the incubation of the egg until the hatching; (b) measuring the amount of the at least first detected developmental marker compound, and (c) comparing the amount to a base line established for male and female, developmental stage of the embryo, and/or alive and deceased or non-developed embryo, to determine whether the embryo is viable, male and/or female, and/or the developmental stage of the embryo.
1 . A system comprising
(a) a quantitative analytic apparatus selected from the group consisting of:
a magnetic resonance spectrometer, a spectral resonance spectrometer, a GC coupled with a suitable detector, an HPLC coupled with a suitable detector, a fluorescence spectrometer, and an enzyme-linked immunosorbent assay system;
for measuring the level of at least a first developmental marker compound selected from sugars and/or amino acids, precursors and metabolites thereof in an egg;
(b) a microprocessor configured to receive data from the quantitative analytic apparatus and programmed to perform multivariate statistical analysis of the level of the at least a first developmental marker to compare the amount to a base line established for male and female, developmental stage of the embryo, and/or alive and deceased or non-developed embryo, to determine whether the embryo is viable, male and/or female, and/or the developmental stage of the embryo and identify viable female and viable male eggs; and
(c) an incubator for subjecting the viable female and/or viable male eggs to an incubation and hatching process to form a predominantly female or male chick population, and/or for subjecting the viable egg selections to an incubation and hatching process in line with the expected hatching date, to form a chick population of predominantly the same developmental stage.
2 . An apparatus for identifying one or more characteristics of an egg at any time during the embryonic development period of an avian embryo in an egg, comprising
(a) a means for detecting at least a first developmental marker compound selected from sugars and/or amino acids, precursors and metabolites thereof in an egg at a time period of from the beginning of the incubation of the egg until the hatching;
(b) a means for measuring the amount of the at least first detected developmental marker compound, and
(c) a means for comparing the amount to a base line established for male and female, developmental stage of the embryo, and/or alive and deceased or non-developed embryo, to determine whether the embryo is viable, male and/or female, and/or the developmental stage of the embryo.
3 . The apparatus according to claim 2 , for non-destructive determination of gender, developmental stage and/or viability of an avian embryo in an egg, further comprising a multivariate statistical analysis program, as well as a microprocessor implemented process for the determination thereof.
4 . The apparatus according to claim 2 , wherein the at least first developmental marker compound is detected in an embryonic fluid of an egg.
5 . The apparatus according to claim 2 , configured to detect at least a second marker by (a), and analysing the at least first and second markers and comparing these to a base line, and to each other to establish a developmental marker ratio.
6 . The apparatus according to claim 2 , wherein the developmental markers for determining whether an embryo is male or female are selected from the groups consisting of Glucose, Choline and/or Valine; and wherein the developmental markers for determining the developmental stage and the time until hatching is likely to occur, and viability are selected from Trimethylglycine; Aspartate; Asparagine; Glutamate, and Proline.
7 . The apparatus according to claim 6 , wherein the avian species is Gallus gallus domesticus , and wherein in the allantois, Glucose is detected in absolute amount for a female embryo in the range of from 30 μM/ml to 70 μM/ml, and for a male embryo of from 1 μM/ml to 30 μM/ml; Choline is present in an absolute amount for a female embryo in the range of from 110 μM/ml to 130 μM/ml, and for a male embryo of from 90 μM/ml up to, but not including 110 μM/ml, and/or Valine is detected in an absolute amount for a female embryo in the range of from 110 μM/ml to 130 μM/ml, and for a male embryo of from 90 μM/ml up to, but not including 110 μM/ml.
8 . The apparatus according to claim 2 , configured to analyze the embryonic fluid invasively or non-invasively.
9 . The apparatus according to claim 8 , configured to perform the analysis by a magnetic resonance imaging method including nuclear resonance methods; by a spectral resonance method including infrared or Raman spectroscopy; and/or an analytical method such as GC or HPLC coupled with suitable detectors, fluorescence spectroscopy and/or an enzyme-linked immunosorbent assay.
10 . The apparatus according to claim 2 , further comprising means for determining whether an embryo in an egg is viable and male, or viable and female, and separating a multitude of viable male eggs from a multitude of viable female eggs, to form a predominantly male or predominantly female egg selection.
11 . A system comprising the apparatus according to claim 10 , further comprising an incubator for subjecting the viable female or male egg selection to an incubation and hatching process to form a predominantly female or male chick population, and/or means for subjecting the viable egg selections to an incubation and hatching process in line with the expected hatching date, to form a chick population of predominantly the same developmental stage.
12 . A system according to claim 11 , further comprising a candling apparatus for determining whether an embryo in an egg is viable, and a means for separating non-viable eggs prior to hatching.
13 . The apparatus according to claim 2 , wherein the at least first developmental marker compound is detected in the allantoic fluid of an egg.
14 . The system according to claim 1 , wherein the quantitative analytic apparatus is selected from the group consisting of:
a magnetic resonance spectrometer, a Raman spectrometer, a Fourier transform infrared (FTIR) spectrometer, a GC coupled with a suitable detector, an HPLC coupled with a suitable detector, a fluorescence spectrometer, and an enzyme-linked immunosorbent assay system.
15 . The apparatus according to claim 2 , wherein the means for detecting and/or the means for measuring is selected from the group consisting of:
a magnetic resonance spectrometer, a Raman spectrometer, a FTIR spectrometer, a GC coupled with a suitable detector, an HPLC coupled with a suitable detector, a fluorescence spectrometer, and an enzyme-linked immunosorbent assay system.