Granted Patent
B2
US 12,649,925 · App. 17/166,285 · Granted Jun 9, 2026
Tissue-specific expression and hybrid plant production
Inventors:
Sandeep Kumar (Carmel, IN); Marcelo Ariel German (Portland, OR); Tristan E Coram (Zionsville, IN); Terry R. Wright (Westfield, IN)
Assignee:
CORTEVA AGRISCIENCE LLC
View Patent ↗
Loading inventors, assignments & file history…
Abstract
This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.
Claims (18)
1 . A transgenic plant cell comprising a recombinant polynucleotide that encodes an AAD-1 protein that exhibits aryloxyalkanoate dioxygenase activity, wherein said activity enzymatically degrades a phenoxy auxin herbicide and an (R)-aryloxyphenoxypropionate herbicide,
wherein, an sRNA target site comprising SEQ ID NO:7, or SEQ ID NO:8 is operably linked to the nucleotide sequence encoding the protein encoded by base pairs 1-888 of SEQ ID NO: 5.
2 . The plant cell of claim 1 wherein the sRNA target site is recognized by an endogenous sRNA of the plant cell.
3 . The plant cell of claim 1 wherein said plant cell is a dicotyledonous or a monocotyledonous plant cell.
4 . A transgenic plant comprising a plurality of the plant cells of claim 1 , wherein expression of said polynucleotide renders said plant tolerant to an aryloxyalkanoate herbicide.
5 . The plant of claim 4 wherein said aryloxyalkanoate herbicide is a phenoxy auxin herbicide.
6 . The plant of claim 4 wherein said aryloxyalkanoate herbicide is selected from the group consisting of 2,4-dichlorophenoxyacetic acid, MCPA, dichlorprop, and mecoprop.
7 . The plant of claim 4 wherein said aryloxyalkanoate herbicide is an (R)-aryloxyphenoxypropionate.
8 . The plant of claim 4 wherein said aryloxyalkanoate herbicide is selected from the group consisting of (R)-fluazifop, (R)-haloxyfop, (R)-diclofop, (R)-quizalofop, (R)-fenoxaprop, (R)-metamifop, (R)-cyhalofop, and (R)-clodinofop.
9 . The plant of claim 4 wherein expression of the AAD-1 protein is reduced or eliminated in at least one tissue of the plant as compared to tissues expressing an AAD-1 mRNA with no sRNA target site.
10 . The plant of claim 4 wherein expression of the AAD-1 protein is reduced or eliminated in tissue-specific or tissue-preferred manner as compared to plants producing AAD-1 mRNA with no target site.
11 . The plant of claim 4 wherein said plant further comprises a second herbicide resistance gene.
12 . The plant of claim 11 wherein said phenoxy auxin herbicide is an R-enantiomer of a chiral phenoxy auxin.
13 . The plant of claim 11 wherein said phenoxy auxin herbicide is an achiral phenoxy auxin selected from the group consisting of 2,4-D and MCPA.
14 . The plant of claim 11 wherein said (R)-aryloxyphenoxypropionate herbicide is selected from the group consisting of (R)-fluazifop, (R)-haloxyfop, (R)-diclofop, (R)-quizalofop, (R)-fenoxaprop, (R)-metamifop, (R)-cyhalofop, and (R)-clodinofop.
15 . A seed comprising a plurality of the plant cells of claim 1 .
16 . A method of controlling weeds in a field, wherein said method comprises applying an aryloxyalkanoate herbicide to said field and planting a seed of claim 15 in said field.
17 . A part, progeny, or asexual propagate grown from the seed of claim 15 , wherein said part, progeny, or asexual propagate comprises said polynucleotide.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 13, 2023
From: KUMAR, SANDEEP; GERMAN, MARCHELO A.; CORAN, TRISTAN E.; WRIGHT, TERRY R.
To: DOW AGROSCIENCES LLC
Reel/Frame 065536/0004 →
CHANGE OF NAME
Recorded Nov 8, 2021
From: DOW AGROSCIENCES LLC
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 058044/0184 →
Continuity (4)
Provisional Application
61922603
· Dec 31, 2013
References Cited (266)
US 5135867A
· Payne et al.
· 1992
[cited by applicant]
US 5273894A
· Strauch et al.
· 1993
[cited by applicant]
US 5316931A
· Donson et al.
· 1994
[cited by applicant]
US 5463175A
· Barry et al.
· 1995
[cited by applicant]
US 5500360A
· Ahlquist et al.
· 1996
[cited by applicant]
US 5561236A
· Leemans et al.
· 1996
[cited by applicant]
US 5589367A
· Donson et al.
· 1996
[cited by applicant]
US 5608147A
· Kaphammer
· 1997
[cited by applicant]
US 5637489A
· Strauch et al.
· 1997
[cited by applicant]
US 5646024A
· Leemans et al.
· 1997
[cited by applicant]
US 5648477A
· Leemans et al.
· 1997
[cited by applicant]
US 5879903A
· Strauch et al.
· 1999
[cited by applicant]
US 5910626A
· Haselkorn et al.
· 1999
[cited by applicant]
US 6087563A
· Dellapenna et al.
· 2000
[cited by applicant]
US 6107549A
· Feng et al.
· 2000
[cited by applicant]
US 6153401A
· Streber et al.
· 2000
[cited by applicant]
US 6268547B1
· Weeks
· 2001
[cited by applicant]
US 6518222B2
· Arndt et al.
· 2003
[cited by applicant]
US 7112665B1
· Leemans et al.
· 2006
[cited by applicant]
US 7250561B1
· Pallett et al.
· 2007
[cited by applicant]
US 7405074B2
· Castle et al.
· 2008
[cited by applicant]
US 7462481B2
· Castle et al.
· 2008
[cited by applicant]
US 7659448B2
· Ahrens et al.
· 2010
[cited by applicant]
US 7838733B2
· Wright
· 2010
[cited by examiner]
US 7863503B2
· Castle et al.
· 2011
[cited by applicant]
US 7998703B2
· Castle et al.
· 2011
[cited by applicant]
US 8222489B2
· Castle et al.
· 2012
[cited by applicant]
US 8598413B2
· Cui et al.
· 2013
[cited by applicant]
US 9074007B2
· Danilevskaya et al.
· 2015
[cited by applicant]
US 9127289B2
· Wright et al.
· 2015
[cited by applicant]
US 20010023501A1
· Johal et al.
· 2001
[cited by applicant]
US 20020059659A1
· Stemmer
· 2002
[cited by applicant]
US 20030041357A1
· Jepson et al.
· 2003
[cited by applicant]
US 20030056245A1
· Chatterjee et al.
· 2003
[cited by applicant]
US 20030135879A1
· Weeks et al.
· 2003
[cited by applicant]
US 20070199095A1
· Allen et al.
· 2007
[cited by applicant]
US 20070300329A1
· Allen et al.
· 2007
[cited by applicant]
US 20090069182A1
· Castle et al.
· 2009
[cited by applicant]
US 20090093366A1
· Wright
· 2009
[cited by examiner]
US 20120054919A1
· Allen et al.
· 2012
[cited by applicant]
US 20120245339A1
· Castle et al.
· 2012
[cited by applicant]
US 20130007908A1
· Huang
· 2013
[cited by examiner]
US 20140325713A1
· Kovalic et al.
· 2014
[cited by applicant]
US 20150184178A1
· Kumar
· 2015
[cited by examiner]
US 20160108422A1
· Ellis et al.
· 2016
[cited by applicant]
AU 2006203892A1
· 2006
[cited by applicant]
AU 2009267007A1
· 2010
[cited by applicant]
CA 2746892A1
· 2010
[cited by applicant]
CN 101466837A
· 2009
[cited by applicant]
CN 102304520A
· 2012
[cited by applicant]
EP 1025250A2
· 2000
[cited by applicant]
EP 1167531A1
· 2002
[cited by applicant]
EP 1824967A2
· 2007
[cited by applicant]
EP 1740039B1
· 2012
[cited by applicant]
EP 2535414A1
· 2012
[cited by applicant]
JP 2005287415A
· 2005
[cited by applicant]
RU 2129005C1
· 1999
[cited by applicant]
WO 8705629A1
· 1987
[cited by applicant]
WO 9013654A1
· 1990
[cited by applicant]
WO 9633270A1
· 1996
[cited by applicant]
WO 9713402A1
· 1997
[cited by applicant]
WO 9802562A2
· 1998
[cited by applicant]
WO 9808963A1
· 1998
[cited by applicant]
WO 9820144A2
· 1998
[cited by applicant]
WO 9838294A1
· 1998
[cited by applicant]
WO 9838336A1
· 1998
[cited by applicant]
WO 9844139A1
· 1998
[cited by applicant]
WO 9910513A1
· 1999
[cited by applicant]
WO 9963092A1
· 1999
[cited by applicant]
WO 0006757A1
· 2000
[cited by applicant]
WO 0009727A2
· 2000
[cited by applicant]
WO 0066748A1
· 2000
[cited by applicant]
WO 0138513A2
· 2001
[cited by applicant]
WO 03013224A2
· 2003
[cited by applicant]
WO 03034813A2
· 2003
[cited by applicant]
WO 03056904A2
· 2003
[cited by applicant]
WO 2005107437A2
· 2005
[cited by applicant]
WO 2005122751A1
· 2005
[cited by applicant]
WO 2006111512A1
· 2006
[cited by applicant]
WO 2007047016A2
· 2007
[cited by applicant]
WO 2007091099A1
· 2007
[cited by applicant]
WO 2008133643A3
· 2009
[cited by applicant]
WO 2011067745A2
· 2011
[cited by applicant]
WO 2013184768A1
· 2013
[cited by applicant]
Chuck et al Development, 2010, 137:1243-1250. (Year: 2010).
[cited by examiner]
Müller et al 2006, Applied and Environmental Microbiology, 72: 4853-4861 (Year: 2006).
[cited by examiner]
White et al 2010, PNAS 107: 20240-20245 (Year: 2010).
[cited by examiner]
Chekan et al 2019, PNAS 116: 13299-13304 (Year: 2019).
[cited by examiner]
Muller et al.2006b (Protein Science 15:1356-1368) (Year: 2006).
[cited by examiner]
IPR2023-01036 (available in unredacted form to Applicant) (Year: 2024).
[cited by examiner]
IPR2023-01037 (available in unredacted form to Applicant) (Year: 2024).
[cited by examiner]
IPR2023-01038 (available in unredacted form to Applicant) (Year: 2024).
[cited by examiner]
Arif M.A., et al., “DICER-LIKE3 Activity in Physcomitrella Patens DICER-LIKE4 Mutants Causes Severe Developmental Dysfunction and Sterility,” Molecular Plant, Nov. 2012, vol. 5, No. 6, pp. 1281-1294, Advance Access Publ…
[cited by applicant]
Chuck G., et al., “The Maize SBP-box Transcription Factor Encoded by Tasselsheath4 Regulates Bract Development and the Establishment of Meristem Boundaries,” Development and Stem Cells, 2010, vol. 137, No. 8, pp. 1243-1…
[cited by applicant]
Extended European Search Report for European Application No. 14876758.5, mailed Sep. 11, 2017, 10 Pages.
[cited by applicant]
International Preliminary Report on Patentability for International Application No. PCT/US2014/073038, mailed Jul. 14, 2016, 12 Pages.
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2014/073038, mailed Apr. 27, 2015, 16 Pages.
[cited by applicant]
Li D., et al., “Deep Sequencing of Maize Small RNAs Reveals a Diverse Set of MicroRNA in Dry and Imbibed Seeds,” PloS one, Jan. 24, 2013, vol. 8, Issue. 1(e55107), 14 Pages.
[cited by applicant]
Yi F., et al., “Genome-Wide Characterization of MicroRNA in Foxtail Millet (
[cited by applicant]
Zhang L., et al., “A Genome-Wide Characterization of MicroRNA Genes in Maize,” PLOS Genetics, Nov. 20, 2009, vol. 5, Issue No. 11(e1000716), 16 Pages.
[cited by applicant]
Wang S., et al., “Development and Validation of Vectors Containing Multiple siRNA Expression Cassettes for Maximizing the Efficiency of Gene Silencing,” BMC Biotechnology, 2006, vol. 6, No. 50, pp. 1-7.
[cited by applicant]
Ohkouchi Y., et al., “Cloning and Expression of DL-2-haloacid Dehalogenase Gene From Burkholderia Cepacia,” Water Science and Technology, 2000, vol. 42, No. 7-8, pp. 261-268.
[cited by applicant]
Park H-D., et al., “Genetic and Phenotypic Diversity of Dichlorprop-Degrading Bacteria Isolated from Soils,” Journal of Microbiology, Seoul, Republic of Korea, 2003, vol. 41, No. 1, pp. 7-15.
[cited by applicant]
Park I.H., et al., “Isolation and Characterization of 4-(2,4-dichlorophenoxy)Butyric Acid-Degrading Bacteria from Agricultural Soils,” Journal of Microbiology and Biotechnology, Jan. 2003, vol. 13, No. 2, pp. 243-250.
[cited by applicant]
Parker L.W., et al., “Kinetics of Microbial Decomposition of 2,4-D in Soil: Effects of Herbicide Concentration,” Journal of Environmental Quality, Oct. 1982, vol. 11, No. 4, pp. 679-684.
[cited by applicant]
Paulin M.M., et al., “Abundance and Expression of Enantioselective rdpA and sdpA Dioxygenase Genes during Degradation of the Racemic Herbicide (R,S)-2-(2,4-Dichlorophenoxy)Propionate in Soil,” Applied and Environmental …
[cited by applicant]
Paulin M.M., et al., “(R,S)-Dichlorprop Herbicide in Agricultural Soil Induces Proliferation and Expression of Multiple Dioxygenase-Encoding Genes in the Indigenous,” Environmental Microbiology, 2011, vol. 13, pp. 1513-…
[cited by applicant]
Perkins E.J., et al., “Use of Alcaligenes Eutrophus as a Source of Genes for 2,4-d Resistance in Plants,” Weed Science, 1987, vol. 35, No. Suppl. 1, pp. 12-18.
[cited by applicant]
Plumeier I., et al., “Importance of Different tfd Genes for Degradation of Chloroaromatics by Ralstonia Eutropha JMP134,” Journal of Bacteriology, Aug. 2002, vol. 184, No. 15, pp. 4054-4064.
[cited by applicant]
Poh R., et al., “2,4-Dichlorophenoxyacetate / .Alpha .- Ketoglutarate Dioxygenases from Burkholderia Cepacia 2a and Ralstonia Eutropha JMP134,” Microbios, 2001, vol. 105, pp. 43-63.
[cited by applicant]
Poh R.P-C., et al., “Complete Characterization of Tn5530 From Burkholderia Cepacia Strain 2a (plJB1) and Studies of 2,4-dichlorophenoxyacetate Uptake by the Organism,” Plasmid, 2002, vol. 48, No. 1, pp. 1-12.
[cited by applicant]
Poiger T., et al., “Environmental Behavior of the Chiral Herbicide Haloxyfop. 1. Rapid and Preferential Interconversion of the Enantiomers in Soil,” Journal of Agricultural and Food Chemistry, 2015, vol. 63, No. 10, pp.…
[cited by applicant]
Preston C., et al., “Multiple Resistance to Dissimilar Herbicide Chemistries in a Biotype OfLolium Rigidumdue to Enhanced Activity of Several Herbicide Degrading Enzymes,” Pesticide Biochemistry and Physiology, 1996, vo…
[cited by applicant]
Prins M., “Broad Virus Resistance in Transgenic Plants,” Trends in Biotechnology, Sep. 1, 2003, vol. 21, No. 9, ISSN: 0167-7799, pp. 373-375.
[cited by applicant]
Rousseaux S., et al., “Isolation and Characterisation of New Gram-negative and Gram-positive Atrazine Degrading Bacteria From Different French Soils,” FEMS Microbiology Ecology, 2001, vol. 36, No. 2-3, pp. 211-222.
[cited by applicant]
Saari R.E., et al., “Stereospecific Degradation of the Phenoxypropionate Herbicide Dichlorprop,” Journal of Molecular Catalysis B: Enzymatic, 1999, vol. 6, No. 4, pp. 421-428.
[cited by applicant]
Sambrook J., et al., “Molecular Cloning,” A Laboratory Manual, Cold Spring Harbor Laboratory Press, Second Edition, 1989, vol. 2, 11.45, 3 Pages.
[cited by applicant]
Schleinitz., et al., “Rhodoferax sp. P230 R-2,4-Dichlorophenoxypropionate Dioxygenase (rdpA) gene,” complete eds., Database EMBL, Accession No. AF516751, Aug. 6, 2002, pp. 1-2.
[cited by applicant]
Schleinitz K.M., et al., “Localization and Characterization of Two Novel Genes Encoding Stereospecific Dioxygenases Catalyzing 2(2,4-Dichlorophenoxy)propionate Cleavage in Delftia acidovorans MC1,” Applied and Environme…
[cited by applicant]
Schleinitz K.M., et al., Sphingobium Hervbicidovorans R-2,4-Dichlorphenoxypropionate Dioxygenase (rdpA) Gene, Complete eds., Database EMBL, Accesion No. AF516752, Aug. 6, 2002, pp. 1-2.
[cited by applicant]
Schneiderheinze J.M., et al., “Plant and Soil Enantioselective Biodegradation of Racemic Phenoxyalkanoic Herbicides,” Chirality, 1999, vol. 11, No. 4, pp. 330-337.
[cited by applicant]
Seifert S., et al., “Effect of Tillage on Microbial Characteristics and Herbicide Degradation in a Sharkey Clay Soil,” Weed Science, 2001, vol. 49, No. 5, pp. 685-693.
[cited by applicant]
Shaw L.J., et al., “Enhanced Mineralization of [U-14C]2,4-dichlorophenoxyacetic Acid in Soil From the Rhizosphere of Trifolium Pratense,” Applied and Environmental Microbiology, Aug. 2004, vol. 70, No. 8, pp. 4766-4774.
[cited by applicant]
Shimojo M., et al., “Analysis of Genes encoding the 2,4-Dichlorophenoxyacetic Acid Degrading Enzyme from Sphingomonas Agrestis 58-1,” Journal of Bioscience and Bioengineering, 2008, vol. 108, No. 1, pp. 56-59.
[cited by applicant]
Smejkal C.W., et al., “Substrate Specificity of chlorophenoxyalkanoic Acid-Degrading Bacteria is not Dependent upon Phylogenetically Related tfdA Gene Types,” Biology and Fertility of Soils, Jun. 2001, vol. 33, No. 6, p…
[cited by applicant]
Spencer T.M., et al., “Segregation of Transgenes in Maize,” Plant Molecular Biology, 1992, vol. 18, pp. 201-210.
[cited by applicant]
Streber W.R., et al., “Analysis, Cloning, and High-Level Expression of 2, 4-Dichlorophenixyacetic Monooxygenase Gene tfdA of Alcaligenes Eutrophus JMP134,” Journal of Bacteriology, 1987, vol. 169, No. 7, pp. 2950-2955.
[cited by applicant]
Streber W.R., et al., “Transgenic Tobacco Plants Expressing a Bacterial Detoxifying Enzyme Are Resistant to 2,4- D,” Bio/Technology, 1989, vol. 7, pp. 811-816, 4 Pages (Abstract Only).
[cited by applicant]
Streber W.R., “Transgenic Tobacco Plants Expressing a Bacterial Detoxifying Enzyme Are Resistant to 2,4-d,” Bio/ Technology, 1989, vol. 7, No. 8, pp. 811-816.
[cited by applicant]
Suwa Y., et al., “Characterization of a Chromosomally Encoded 2,4-Dichlorophenoxyacetic Acid/Ketoglutarate Dioxygenase from Burkholderia sp. Strain RASC,” Applied and Environmental Microbiology, Jul. 1996, vol. 62, No. …
[cited by applicant]
Swiegers J.H., et al., “Carnitine Biosynthesis in Neurospora Crassa: Identification of a cDNA Coding for Epsilon-n- trimethyllysine Hydroxylase and Its Functional Expression in Saccharomyces Cerevisiae,” FEMS Microbiolo…
[cited by applicant]
Tamura K., “Microbial Pesticide Degradations and Evolutionary Analysis of Degrading Enzymes,” Nippon Noyaku Gakkaishi, 2001, vol. 26, No. 3, pp. 309-314, 9 Pages.
[cited by applicant]
Tett V.A., et al., “Biodegradation of the Chlorophenoxy Herbicide (R)-(+)-Mecoprop by Alcaligenes Denitrificans,” Biodegradation, 1997, vol. 8, No. 1, pp. 43-52.
[cited by applicant]
Tett V.A., et al., “Enantioselective Degradation of the Herbicide Mecoprop [2-(2-methyl-4-chlorophenoxy) Propionic Acid] by Mixed and Pure Bacterial Cultures,” FEMS Microbiology Ecology, 1994, vol. 14, No. 3, pp. 191-20…
[cited by applicant]
Top E.M., et al., “Capture of a Catabolic Plasmid That Encodes Only 2,4-dichlorophenoxyacetic Acid:alpha- ketoglutaric Acid Dioxygenase (Tfda) by Genetic Complementation,” Applied and Environmental Microbiology, Jul. 19…
[cited by applicant]
Top E.M., et al., “Methane Oxidation as a Method to Evaluate the Removal of 2,4-dichlorophenoxyactic Acid (2,4-d) From Soil by Plasmid-mediated Bioaugmentation,” FEMS Microbiology Ecology, 1999, vol. 28, No. 3, pp. 203-…
[cited by applicant]
Top E.M., et al., “The Role of Mobile Genetic Elements in Bacterial Adaptation to Xenobiotic Organic Compounds,” Current Opinion in Biotechnology, 2003, No. 14, No. 3, pp. 262-269.
[cited by applicant]
Travkin V.M., et al., “Characterization of an Intradiol Dioxygenase Involved in the Biodegradation of the Chlorophenoxy Herbicides 2,4-d and 2,4,5-t,” FEBS Letters, 1997, vol. 407, No. 1, pp. 69-72.
[cited by applicant]
UNIPROT: “R-2,4-Dichlorophenoxypropionate Dioxygenase ((R)-2-(2,4-Dichlo Oxoglutarate Dioxygenase,” Database Accession No. Q8KSC8.2, RdpA Shingobium herbicidovorans, Sequence updated: Mar. 1, 2004, Oct. 31, 2006, 2 page…
[cited by applicant]
Vallaeys T., et al., “Isolation and Characterization of a Stable 2,4-Dichlorophenoxyacetic Acid Degrading Bacterium, Variovorax Paradoxus, using Chemostat Culture,” Biotechnology Letters, Nov. 1998, vol. 20, No. 11, pp.…
[cited by applicant]
Vallaeys T., et al., “PCR-RFLP Analysis of 16s rRNA, tfdA and tfdb Genes Reveals a Diversity of 2,4-d Degraders in Soil Aggregates,” FEMS Microbiology Ecology, 1997, vol. 24, No. 3, pp. 269-278.
[cited by applicant]
Vallaeys T., et al., “The Metabolic Pathway of 2,4-dichlorophenoxyacetic Acid Degradation Involves Different Families of tfda and tfdb Genes According to PCR-RFLP Analysis,” FEMS Microbiology Ecology, 1996, vol. 20, No.…
[cited by applicant]
Vedler E., et al., “Analysis of the 2,4-dichlorophenoxyacetic Acid-degradative Plasmid pEST4011of Achromobacter Xylosoxidans Subsp. Denitrificans Strain EST4002,” Gene, 2000, vol. 255, No. 2, pp. 281-288.
[cited by applicant]
Vedler E., et al., “TfdR, the LysR-type Transcriptional Activator, is Responsible for the Activation of the tfdCB Operon of Pseudomonas Putida 2,4-Dichlorophenoxyacetic Acid Degradative Plasmid pEST4011,” Gene, 2000, vo…
[cited by applicant]
Vedler E., et al., “The Completely Sequenced Plasmid pEST4011 Contains a Novel IncP1 Backbone and a Catabolic Transposon Harboring tfd Genes for 2,4-Dichlorophenoxyacetic Acid Degradation,” Journal of Bacteriology, Nov.…
[cited by applicant]
Velicer G.J., “Pleiotropic Effects of Adaptation to a Single Carbon Source for Growth on Alternative Substrates,” Applied and Environmental Microbiology, Jan. 1999, vol. 65, No. 1, pp. 264-269.
[cited by applicant]
Westendorf A., et al., “Kinetic Traits and Enzyme Form Patterns of (R)-2-(2,4-Dichlorophenoxy) propionate/Alpha-Ketoglutarate Dioxygenase (RdpA) after Expression in Different Bacterial Strains,” Engineering in Life Scie…
[cited by applicant]
Westendorf A., et al., “Purification and Characterisation of the Enantiospecific Dioxygenases from Delftia acidovorans MC1 Initiating the Degradation of Phenoxypropionate and Phenoxyacetate Herbicides,” Jan. 1, 2003, vo…
[cited by applicant]
Westendorf A., et al., “The Two Enantiospecific Dichlorprop/Alpha-Ketoglutarate-Dioxygenases from Delftia Acidovorans MC1protein and Sequence Data of RdpA and SdpA,” Microbiological Research, 2002, vol. 157, pp. 317-322.
[cited by applicant]
Westendorf A., et al., “The Two Enantiospecific Dichlorprop/.Alpha.—Ketoglutarate-Dioxygenases from Delftia Acidovorans MC1Protein and Sequence Data of RdpA and SdpA,” Microbiological Research, 2002, vol. 157, pp. 317-3…
[cited by applicant]
Whiting A.K., et al., “Metal Coordination Environment of a Cu(II)-Substituted -Keto Acid-Dependent Dioxygenase That Degrades the Herbicide 2,4-D,” Journal of the American Chemical Society, 1997, vol. 119, No. 14, pp. 34…
[cited by applicant]
Wu X., et al., “Rapid Biodegradation of the Herbicide 2,4- Dichlorophenoxyacetic Acid by Cupriavidus Gilardii T-I,” Journal of Agricultural and Food Chemistry, 2017, vol. 65, No. 18, pp. 3711-3720.
[cited by applicant]
Yi H-R., et al., “Phylogenetic and Phenotypic Diversity of 4-chlorobenzoate-degrading Bacteria Isolated From Soils,” FEMS Microbiology Ecology, 2000, vol. 31, No. 1, pp. 53-60.
[cited by applicant]
Zabaloy M.C., et al., “Isolation and Characterization of Indigenous 2,4-D Herbicide Degrading Bacteria from an Agricultural Soil in Proximity of Sauce Grande River, Argentina,” Annals of Microbiology, 2014, vol. 64, No.…
[cited by applicant]
Zaprasis A., et al., “Abundance of Novel and Diverse tfdA-Like Genes, Encoding Putative Phenoxyalkanoic Acid Herbicide-Degrading Dioxygenases, in Soil,” Applied and Environmental Microbiology, Jan. 2010, vol. 76, No. 1,…
[cited by applicant]
Zhang B-H., et al., “In Vitro Assay for 2,4-D Resistance in Transgenic Cotton,” In Vitro Cellular & Developmental Biology: Plant, March-Apr. 2001, vol. 37, No. 2, pp. 300-304.
[cited by applicant]
Zhang B-H., et al., “Inheritance and Segregation of Exogenous Genes in Transgenic Cotton,” Journal of Genetics, Aug. 2000, vol. 79, No. 2, pp. 71-75.
[cited by applicant]
Zhang H., et al., “Two Dcm Gene Clusters Essential for the Degradation of Diclofopmethyl in a Microbial Consortium of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9,” Journal of Agricultural and Food Chemistry, 2018, v…
[cited by applicant]
Zhang L., et al., “Enantioselective Catabolismof the Two Enantiomers of the Phenoxyalkanoic Acid Herbicide Dichlorprop by Sphingopyxis sp. DBS4,” Journal of Agricultural and Food Chemistry, 2020, vol. 68, No. 26, ISSN: …
[cited by applicant]
Zhang Y., et al., “Enantioselective Environmental Behavior of the Chiral Herbicide Fenoxaprop-Ethyl and Its Chiral Metabolite Fenoxaprop in Soil,” Journal of Agricultural and Food Chemistry, 2010, vol. 58, No. 24, pp. 1…
[cited by applicant]
Zipper C., et al., “Complete Microbial Degradation Of Both Enantiomers Of The Chiral Herbicide Mecoprop [(Rs)-2- (4-chloro-2-methylphenoxy)Propionic Acid] in an Enantioselective Manner By Sphingomonas herbicidovorans Sp…
[cited by applicant]
Zipper C., et al., “Enantioselective Uptake and Degradation of the Chiral Herbicide Dichlorprop [(Rs)-2-(2,4- dichlorophenoxy)propanoic Acid] by Sphingomonas Herbicidovorans MH,” Journal of Bacteriology, Jul. 1998, vol.…
[cited by applicant]
Baelum J., et al., “Comparison of 16S rRNA Gene Phylogeny and Functional tfdA Gene Distribution in Thirty-One Different 2, 4-Dichlorophenoxyacetic Acid and 4-Chloro-2-Methylphenoxyacetic Acid Degraders,” Systematic and …
[cited by applicant]
Baelum J., et al., “Degradation of 4-Chloro-2-Methylphenoxyacetic Acid in Top- and Subsoil is Quantitatively Linked to the Class III tfdA Gene,” Applied and Environmental Microbiology, Feb. 2006, vol. 72, pp. 1476-1486.
[cited by applicant]
Baelum J., et al., “TaqMan Probe-Based Real-Time PCR Assay for Detection and Discrimination of Class I, II, and III tfdA Genes in Soils Treated with Phenoxy Acid Herbicides,” Applied and Environmental Microbiology, May …
[cited by applicant]
Bayley C., et al., “Engineering 2,4-D Resistance Into Cotton,” Theoretical and Applied Genetics, Jan. 1, 1992, vol. 83, No. 5, pp. 645-649.
[cited by applicant]
Bhat M.A., et al., “Purification of 3,5-dichlorocatechol 1,2-dioxygenase, a Nonheme Iron Dioxygenase and a Key Enzyme in the Biodegradation of a Herbicide, 2,4-dichlorophenoxyacetic Acid (2,4-d), From Pseudomonas Cepaci…
[cited by applicant]
Bisht N.C., et al., “Development of 2,4-O-Resistant Transgenics in Indian Oilseed Mustard (Brassica Juncea),” Current Science, Aug. 10, 2004, vol. 87, No. 3, ISSN: 0011-3891, pp. 367-370.
[cited by applicant]
Chaudhry G.R., et al., “Isolation and Characterization of a New Plasmid From a Flavobacterium Sp. Which Carries the Genes for Degradation of 2,4-dichlorophenoxyacetate,” Journal of Bacteriology, 1988, vol. 170, No. 9, p…
[cited by applicant]
Chen S., et al., “Reasons for the Acclimation for 2,4-D Biodegradation in Lake Water,” Journal of Environmental Quality, Apr. 1989, vol. 18, No. 2, pp. 153-156.
[cited by applicant]
Cho S.H., et al., “Isolation and Characterization of 2-Methyl-4-Chlorophenoxyacetic Acid-Degrading Bacteria from Agricultural Soils,” Agricultural Chemistry and Biotechnology (English Edition), 1999, vol. 42, No. 2, pp.…
[cited by applicant]
Cosper N.J., et al., “X-ray Absorption Spectroscopic Analysis of Fe(II) and Cu(II) Forms of a Herbicide-degrading Alpha-ketoglutarate Dioxygenase,” Journal of Biological Inorganic Chemistry, 1999, vol. 4, No. 1, pp. 122…
[cited by applicant]
Daane L.L., et al., “Earthworm Egg Capsules as Vectors for the Environmental Introduction of Biodegradative Bacteria,” Applied and Environmental Microbiology, Jun. 1999, vol. 65, No. 6, pp. 2376-2381.
[cited by applicant]
Dellaporta S.L., et al., “A Plant DNA Minipreparation: Version 11,” Plant Molecular Biology Reporter, Jan. 1, 1983, vol. 1, No. 4, ISSN: 0735-9640, pp. 19-21.
[cited by applicant]
Don R.H., et al., “Genetic and Physical Map of the 2,4-Dichlorophenoxyacetic Acid-Degradative Plasmid pJP4,” Journal of Bacteriology, Jan. 1985, vol. 161, No. 1, pp. 466-468.
[cited by applicant]
Donr.H., et al., “Transposon Mutagenesis and Cloning Analysis of the Pathways for Degradation of 2,4- Dichlorophenoxyacetic Acid and 3-Chlorobenzoate in Alcaligenes Eutrophus JMP134(pJP4),” Journal of Bacteriology, Jan.…
[cited by applicant]
“sdpA MC1 Delftia Aciovorans,” UniProtKB Accession No. P83309.2, Sep. 18, 2019, 3 Pages.
[cited by applicant]
Dunbar J., et al., “Genetic Diversity Through the Looking Glass: Effect of Enrichment Bias,” Applied and Environmental Microbiology, Apr. 1997, vol. 63, No. 4, pp. 1326-1331.
[cited by applicant]
Ehrig A., et al., “Isolation of Phenoxy Herbicide-Degrading Rhodoferax Species from Contaminated Building Material,” Acta Biotechnologica, 1997, vol. 17, No. 4, pp. 351-356.
[cited by applicant]
Eichhorn E., et al., “Characterization of Alpha-Ketoglutarate-Dependent Taurine Dioxygenase from Escherichia Coli,” Journal of Biological Chemistry, Sep. 1997, vol. 272, No. 37, ISSN: 0021-9258, p. 23031-23036.
[cited by applicant]
Feng L., et al., Biodegradation and Plant Protection From the Herbicide 2,4-D by Plant-microbial Associations in Cotton Production Systems, Biotechnology and Bioengineering, 1997, vol. 54, No. 6, pp. 513-519.
[cited by applicant]
Fuchslin H.P., et al., “Effect of Integration of a Gfp Reporter Gene on Fitness of Ralstonia Eutropha During Growth With 2,4-dichlorophenoxyacetic Acid,” Environmental Microbiology, Oct. 2003, vol. 5, No. 10, pp. 878-88…
[cited by applicant]
Fukamori F., et al., Alcaligenes Eutrophus JMP134 “2,4-Dichlorophenoxyacetate Monooxygenase is an a-Ketoglutarate-Dependent Dioxygenase,” Journal of Bacteriology, 1993, vol. 175, pp. 2083-2086.
[cited by applicant]
Fukamori F., et al., “Purification and Characterization of 2,4-Dichlorophenoxyacetate/Alpha-Ketoglutarate Dioxygenase,” Journal of Biological Chemistry, 1993, vol. 268, No. 32, pp. 24311-24317.
[cited by applicant]
Gazitua M.C., et al., “Novel Alpha-Ketoglutarate Dioxygenase tfdA-Related Genes are found in Soil DNA after Exposure to Phenoxyalkanoic Herbicides,” Environmental Microbiology, 2010, vol. 12, No. 9, pp. 2411-2425.
[cited by applicant]
GenBank: “(S)-2-(2,4-dichlorophenoxy)propionate/alpha-ketoglutarate dioxygenase [Rhodoferax sp. P230],” GenBank Accession No. ABD67501.1, Mar. 14, 2006, 2 Pages.
[cited by applicant]
Griffin S.L., et al., “Characterization Of Aryloxyalkanoate Dioxygenase-12, A Nonheme Fe(II)/Alpha.-Ketoglutarate- Dependent Dioxygenase, Expressed in Transgenic Soybean and Pseudomonas Fluorescens,” Journal of Agricult…
[cited by applicant]
Han L., et al., “16S rRNA Gene Phylogeny and tfdA Gene Analysis of 2,4-D-Degrading Bacteria Isolated in China,” World Journal of Microbiology & Biotechnology, 2014, vol. 30, No. 10, pp. 2567-2576.
[cited by applicant]
Han L., et al., “Cloning, Expression, Characterization and Mutational Analysis of the tfdA Gene from Cupriavidus Campinensis BJ71,” World Journal of Microbiology & Biotechnology, 2015, vol. 31, No. 7, pp. 1021-1030.
[cited by applicant]
Harker A.R., et al., “Phenoxyacetic Acid Degradation by the 2,4-dichlorophenoxyacetic Acid (TFD) Pathway of Plasmid pJP4: Mapping and Characterization of the TFD Regulatory Gene, tfdR,” Journal of Bacteriology, 1989, vo…
[cited by applicant]
Hausinger R.P., “Biochemical Diversity of 2-0xoglutarate-Dependent Oxygenases,” 2015, vol. 3, No. 1, pp. 1-58.
[cited by applicant]
Hausinger R.P., et al., “Characterization of the First enzyme in 2,4-Dichlorphenoxyacetic Acid Metabolism,” Environmental Health Perspectives, Jun. 1995, vol. 103, Supplement 5, pp. 37-39.
[cited by applicant]
Hausinger R.P., et al., “Fe(II)/Alpha-Ketoglutarate-Dependent Hydroxylases and Related Enzymes,” Critical Reviews in Biochemsitry, 2004, vol. 39, No. 1, pp. 21-68, 51 Pages.
[cited by applicant]
Hawkins A.C., et al., “Chemotaxis of Ralstonia eutropha JMP134(pJP4) to the Herbicide 2,4- dichlorophenoxyacetate,” Applied and Environmental Microbiology, Feb. 2002, vol. 68, No. 2, pp. 968-972.
[cited by applicant]
Hegg E.L., et al., “Herbicide-Degrading Alpha-Keto Acid-Dependent Enzyme TfdA: Metal Coordination Environment and Mechanistic Insights,” Biochemistry, Dec. 14, 1999, vol. 38, No. 50, ISSN 0006-2960, XP002549095, p. 1671…
[cited by applicant]
Hoffmann D., et al., “A Transposon Encoding the Complete 2,4-dichlorophenoxyacetic Acid Degradation Pathway in the Alkalitolerant Strain Delftia Acidovorans P4a,” Microbiology, 2003, vol. 149, No. 9, pp. 2545-2556.
[cited by applicant]
Hoffmann D., et al., “Development and Application of PCR Primers for the Detection of the Lid Genes in Delftia Acidovorans P4a Involved in the Degradation of 2,4-D,” Acta Biotechnologica, 2001, vol. 21, No. 4, pp. 321-3…
[cited by applicant]
Hogan D.A., et al., “Distribution of the Tfda Gene in Soil Bacteria That Do not Degrade 2,4-dichlorophenoxyacetic Acid (2,4-d),” Microbial Ecology, 1997, vol. 34, No. 2, pp. 90-96.
[cited by applicant]
Hogan D.A., et al., “Site-directed Mutagenesis of 2,4-dichlorophenoxyacetic Acid/alpha-ketoglutarate Dioxygenase. Identification of Residues Involved in Metallocenter Formation and Substrate Binding,” Journal of Biologi…
[cited by applicant]
Horvath M., et al., “Isolation and Characterization of a 2-(2,4-dichlorophenoxy) Propionic Acid-degrading Soil Bacterium,” Applied Microbiology and Biotechnology, 1990, vol. 33, pp. 213-216, 1 Page (Abstract only).
[cited by applicant]
Hotopp J.C.D., et al., “Alternative Substrates of 2,4-Dichlorophenoxyacetate/a-Ketoglutarate Dioxygenase,” Journal of Molecular Catalysis B: Enzymatic, Nov. 1, 2001, vol. 15, No. 4-6, pp. 155-162.
[cited by applicant]
Hotopp J.C.D., et al., “Probing the 2,4-Dichlorophenoxyacetate/Alpha-Ketoglutarate Dioxygenase Substrate-Binding Site by Site-Directed Mutagenesis and Mechanism-Based Inactivation,” Biochemistry, 2002, vol. 41, No. 31, …
[cited by applicant]
Hulse J.D., et al., “Carnitine Biosynthesis Beta-Hydroxylation of Tri Methyl Lysine by an Alpha-Keto Glutarate Dependent Mitochondrial Dioxygenase,” Journal of Biological Chemistry, Mar. 10, 1978, vol. 253, No. 5, ISSN:…
[cited by applicant]
Itoh K., et al., “Root Nodule Bradyrhizobium spp. Harbor tfdA and cadA, Homologous with Genes Encoding 2,4- Dichlorophenoxyacetic Acid-Degrading Proteins,” Applied and Environmental Microbiology, 2004, vol. 70, No. 4, p…
[cited by applicant]
Itoh K., et al., “tfdA-like Genes in 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia Cluster in Proteobacteria,” Applied and Environmental Microbiology, Jul…
[cited by applicant]
Jackman S.A., et al., “Electrokinetic Movement and Biodegradation of 2,4-dichlorophenoxyacetic Acid in Silt Soil,” Biotechnology and Bioengineering, Jul. 5, 2001, vol. 74, No. 1, pp. 40-48.
[cited by applicant]
Jing X., et al., “Enantioselective Toxicity and Degradation of Chiral Herbicidefenoxaprop-Ethyl in Earthworm Eisenia Fetida,” Ecological indicators, 2017, vol. 75, pp. 126-131.
[cited by applicant]
Ka J.O., et al., “Integration and Excision of a 2,4-dichlorophenoxyacetic Acid-degradative Plasmid in Alcaligenes Paradoxus and Evidence of Its Natural Intergeneric Transfer,” Journal of Bacteriology, Sep. 1994, vol. 17…
[cited by applicant]
Ka J.O., et al., “Use of Gene Probes to Aid in Recovery and Identification of Functionally Dominant 2,4- dichlorophenoxyacetic Acid-degrading Populations in Soil,” Applied and Environmental Microbiology, Apr. 1994, vol.…
[cited by applicant]
Kaneko T., et al., “Complete Genomic Sequence of Nitrogen-Fixing Symbiotic Bacterium Bradyrhizobium Japonicum USDA110,” Dna Research, 2002, vol. 9, pp. 189-197.
[cited by applicant]
Kaneko T., et al., “Complete Genomic Sequence of Nitrogen-fixing Symbiotic Bacterium Bradyrhizobium Japonicum USDA110 (Supplement),” DNA Research, Nov. 18, 2002, vol. 9, No. 6, pp. 225-256 (Supplement).
[cited by applicant]
Kitagawa W., et al., “Novel 2,4-Dichlorophenoxyacetic Acid Degradation Genes From Oligotrophic Bradyrhizobium Sp. Strain HW13 Isolated From a Pristine Environment,” Journal of Bacteriology, Jan. 2002, vol. 184, No. 2, p…
[cited by applicant]
Kleinsteuber S., et al., “Expression of the 2,4-d Degradative Pathway of pJP4 in an Alkaliphilic, Moderately Halophilic Soda Lake Isolate, Halomonas Sp. EF43,” Extremophiles, 2001, vol. 5, No. 6, pp. 375-384.
[cited by applicant]
Kohler H.P., et al., “Sphingomonas Herbicidovorans MH: a Versatile Phenoxyalkanoic Acid Herbicide Degrader,” Genbank Accession No. Q8KSC8, Oct. 31, 2006, 2 Pages, First Published on Oct. 1, 2002.
[cited by applicant]
Kohler H.P.E., “Sphingomonas Herbicidovorans Mh: A Versatile Phenoxyalkanoic Acid Herbicide Degrader,” Journal of Industrial Microbiology and Biotechnology, 1999, vol. 23, XP002587234, pp. 336-340.
[cited by applicant]
Kohler, “Sphingomonas Herbicidovorans tnpA Gene for Transposase and rdpA Gene for (R)-2- (2,4- Dichlorophenoxy) Propionate, 2-Oxoglutarate Dioxygenase,” Database EMBL, Accesion No. AJ628859, Mar. 3, 2004, pp. 1-2.
[cited by applicant]
Kraube J., et al., “Crystal Structure of the 2-Oxoglutarate Dependent Dioxygenase RdpA,” 4th Biotechnology Symposium of the University of Leipzig (Abstract), Protein Engineering and Bioanalytics, 2005, 1 Page.
[cited by applicant]
Kwon J.K., et al., “Expression of 2,4-Dichlorophenoxyacetic Acid Monooxygenase Gene in Tobacco Plants,” Journal of Korean Society of Horticultural Science, 1997, vol. 38, No. 6, pp. 796-799.
[cited by applicant]
Laemmli C., et al., “Mutation Analysis of the Different Tfd Genes for Degradation of Chloroaromatic Compounds in Ralstonia Eutropha JMP134,” Archives of Microbiology, 2004, vol. 181, No. 2, pp. 112-121.
[cited by applicant]
Larue C.T., et al., “Development of Enzymes for Robust Aryloxyphenoxypropionate and Synthetic Auxin Herbicide Tolerance Traits in Maize and Soybean Crops,” Pest Management Science, 2019, vol. 75, No. 8, pp. 2086-2094.
[cited by applicant]
Laurent F., et al., “2,4-Dichlorophenoxyacetic Acid Metabolism in Transgenic Tolerant Cotton (Gossypium hirsutum),” Journal of Agricultural and Food Chemistry, 2000, vol. 48, No. 11, pp. 5307-5311.
[cited by applicant]
Leibeling S., et al., “Posttranslational Oxidative Modification of (R)-2- (2,4-dichlorophenoxy) Propionate/Alpha- Ketoglutarate-Dependent Dioxygenases (RdpA) Leads to Improved Degradation of 2,4-Dichlorophenoxyacetate (…
[cited by applicant]
Letouze A., et al., “Enhanced Activity of Several Herbicide-degrading Enzymes: a Suggested Mechanism Responsible for Multiple Resistance in Blackgrass (Alopecurus Myosuroides Huds.),” Agronomie, 2003, vol. 23, No. 7, pp…
[cited by applicant]
Lewis D.L., et al., “Influence of Environmental Changes on Degradation of Chiral Pollutants in Soils,” Nature, 1999, vol. 401, No. 6756, pp. 898-901.
[cited by applicant]
Lim J-S., et al., “Genetic and Phenotypic Diversity of (R/S)-Mecoprop [2-(2-Methyl-4-Chlorophenoxy)Propionic acid]- Degrading Bacteria Isolated from Soils,” Journal of Microbiology, Seoul, Republic of Korea, 2004, vol. …
[cited by applicant]
Lipthay J.R.D., et al., “Enhanced Degradation of Phenoxyacetic Acid in Soil by Horizontal Transfer of the Tfda Gene Encoding a 2,4-dichlorophenoxyacetic Acid Dioxygenase,” FEMS Microbiology Ecology, 2001, vol. 35, No. 1…
[cited by applicant]
Lipthay J.R.D., et al., “Expression of Tfda Genes in Aquatic Microbial Communities During Acclimation to 2,4- Dichlorophenoxyacetic Acid,” FEMS Microbiology Ecology, 2002, vol. 40, No. 3, pp. 205-214.
[cited by applicant]
Lipthay J.R.D., et al., “In Situ Exposure to Low Herbicide Concentrations Affects Microbial Population Composition and Catabolic Gene Frequency in an Aerobic Shallow Aquifer,” Applied and Environmental Microbiology, Jan…
[cited by applicant]
Liu Y.J., et al., “Consumers of 4-Chloro-2-Methylpheoxyacetic Acid from Ggricultural Soil and Drilosphere Harbor cadA, r/sdpA, and tfdA-like Gene Encoding Oxygenases,” FEMS Microbiology Ecology, 2013, vol. 86, No. 1, pp…
[cited by applicant]
Llewellyn D., et al., “Genetic Engineering of Crops for Tolerance to 2,4-D,” Herbicide-Resistant Crops, 1996, Chapter 10, pp. 159-174.
[cited by applicant]
Loos M.A., et al., “Phenoxyacetate Herbicide Detoxication by Bacterial Enzymes,” Journal of Agricultural and Food Chemistry, 1967, vol. 15, No. 5, pp. 858-860.
[cited by applicant]
Ludwig P., et al., “Chromatographic Separation of the Enantiomers of Marine Pollutants. Part 5: Enantioselective Degradation of Phenoxycarboxylic Acid Herbicides by Marine Microorganisms,” Chemosphere, 1992, vol. 24, No…
[cited by applicant]
Lyon B.R., et al., “Cotton Plants Transformed with a Bacterial Degradation Gene are Protected from Accidental Spray Drift Damage by the Herbicide 2,4-Dichlorophenoxyacetic Acid,” Transgenic Research, 1993, vol. 2, No. 3…
[cited by applicant]
Lyon B.R., et al., “Expression of a Bacterial Gene in Transgenic Tobacco Plants Confers Resistance to the Herbicide 2,4-dichlorophenoxyacetic Acid,” Plant Molecular Biology, 1989, vol. 13, No. 5, pp. 533-540.
[cited by applicant]
Mae A.A., et al., “Characterization of a New 2,4-dichlorophenoxyacetic Acid Degrading Plasmid pEST4011: Physical Map and Localization of Catabolic Genes,” Journal of General Microbiology, 1993, vol. 139, No. 12, pp. 316…
[cited by applicant]
Maltseva O., et al., “Degradation of 2,4-dichlorophenoxyacetic Acid by Haloalkaliphilic Bacteria,” Microbiology, 1996, vol. 142, No. 5, pp. 1115-1122.
[cited by applicant]
Marriott M.W., et al., “Biodegradation of Mixtures of Chlorophenoxyalkanoic Acid Herbicides by Alcaligenes Denitrificans,” Journal of Industrial Microbiology & Biotechnology, 2000, vol. 25, No. 5, pp. 255-259.
[cited by applicant]
Matheson V.G., et al., “Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/ (alpha)-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp,” Applied and Environmental Microbiology, …
[cited by applicant]
McGowan C., et al., “Evidence for Interspecies Gene Transfer in the Evolution of 2,4- Dichlorophenoxyacetic Acid Degraders,” Applied and Environmental Microbiology, Oct. 1998, vol. 64, No. 10, pp. 4089-4092.
[cited by applicant]
Mierzejewska E., et al., “Biodegradation Potential and Ecotoxicity Assessment in Soil Extracts Amended with Phenoxy Acid Herbicide (2,4-D) and a Structurally-Similar Plant Secondary Metabolite (Ferulic Acid),” Bulletin …
[cited by applicant]
Mortensen S.K., et al., “Influence of Frozen Storage on Herbicide Degradation Capacity in Surface and Subsurface Sandy Soils,” Environmental Science & Technology, 2004, vol. 38, No. 24, pp. 6625-6632.
[cited by applicant]
Mueller R.H., et al., “Degradability and Recalcitrance of Phenoxyalkanoic Acid Herbicides—Traits of the Microbial Metabolism,” In Second International Conference on Remediation of Chlorinated and Racalcitrant Compounds,…
[cited by applicant]
Mueller T.A., et al., “Insights into the Enantiospecificities of (R)- and (S)-Dichlorprop/a-Ketoglutarate Dioxygenases,” Un Mich ICBIC, 2005, 3 Pages (Abstract).
[cited by applicant]
Muller R.H., “Activity and Reaction Mechanism of the Initial Enzymatic Step Specifying the Microbial Degradation of 2,4-Dichlorophenoxyacetate,” Engineering in Life Sciences, 2007, vol. 7, No. 4, pp. 311-321.
[cited by applicant]
Muller R.H., et al., “Comamonas Acidovorans Strain Mc1. A New Isolate Capable of Degrading the Chiral Herbicides Dichlorprop and Mecoprop and the Herbicides 2,4-d and Mcpa,” Microbiological Research, 1999, vol. 154, No.…
[cited by applicant]
Muller R.H., et al., “Separation of Two Dichlorprop/a-ketoglutarate Dioxygenases With Enantiospecific Properties From Comamonas Acidovorans Mc1,” Acta Biotechnologica, 1999, vol. 19, No. 4, pp. 349-355.
[cited by applicant]
Muller R.H., et al., “Physiological and Genetic Characteristics of Two Bacterial Strains Utilizing Phenoxypropionate and Phenoxyacetate Herbicides,” Microbiological Research, 2001, vol. 156, No. 2, pp. 121-131.
[cited by applicant]
Muller R.H., et al., “Pseudo-Recalcitrance of Chlorophenoxyalkanoate Herbicides—Correlation tothe Availability of Ketoglutarate,” Acta Biotechnologica, 2001, vol. 21, No. 3, pp. 227-242.
[cited by applicant]
Muller T.A., et al., “Genetic Analysis of Phenoxyalkanoic Acid Degradation in Sphingomonas Herbicidovorans MH,” Applied and Environmental Microbiology, Oct. 2004, vol. 70, No. 10, pp. 6066-6075.
[cited by applicant]
Muller T.A., “Metabolism of Phenoxyalkanoic Acid Herbicides in Sphingomonas Herbicidovorans Mh Cloning And Characterization of Two Enantiospecific -ketoglutarate-dependent Dioxygenases and Degradation Pathway Analysis,”…
[cited by applicant]
Ncbi: “2,4-D Dioxygenase (Plasmid) [Burkholderia Cepacia],” GenBank Accession No. AAB47567.1, Jul. 26, 2016, 1 Page.
[cited by applicant]
NCBI: “2,4-D/Alpha-ketoglutarate Dioxygenase [Delftia Acidovorans],” GenBank Accession No. AAM76772.1, Jul. 26, 2016, 2 Pages.
[cited by applicant]
NCBI: “2,4-D/Alpha-ketoglutarate Dioxygenase (Plasmid) [Burkholderia Cepacia],” GenBank Accession No. AAK81681.1, Jul. 23, 2016, 2 Pages.
[cited by applicant]
NCBI: “2,4-Dichlorophenoxyacetate Monooxygenase (Gtg Start Codon) [Cupriavidus Necator],” GenBank Accession No. AAA21983.1, Apr. 26, 1993, 1 Page.
[cited by applicant]
NCBI: “A. eutrophus 2, Dichlorophenoxyacetate Monooxygenase Gene, Complete Cds,” GenBank Database Accession No. M16730, Apr. 26, 1993, 2 Pages.
[cited by applicant]
NCBI: “Alpha KG dependent 2,4-D Dioxygenase [Alpha Proteobacterium HW13],” GenBank Accession No. BAB92966.1, Jul. 2, 2002, 1 page.
[cited by applicant]
NCBI: “Alpha KG dependent 2,4-D Dioxygenase [Alpha Proteobacterium HWK12],” GenBank Accession No. BAB92965.1, Jul. 2, 2002, 1 Page.
[cited by applicant]
NCBI: “Alpha KG dependent 2,4-D Dioxygenase [Alpha Proteobacterium RD5-C2],” GenBank Accession No. BAB92964.1, Jul. 2, 2002, 1 Page.
[cited by applicant]
NCBI: “RecName: Full=Alpha-ketoglutarate-dependent 2,4-dichlorophenoxyacetate Dioxygenase; Short=2,4-D Dioxygenase,” UniProtKB Accession No. Q45423.1, Aug. 12, 2020, 2 Pages.
[cited by applicant]
NCBI: “TfdA (Plasmid) [Achromobacter Denitrificans],” GenBank Accession No. AAS49436.1, Apr. 3, 2020, 2 Pages.
[cited by applicant]
Nickel K., et al., “Involvement of Two Alpha-ketoglutarate-dependent Dioxygenases in Enantioselective Degradation of (R)- and (S)-mecoprop by Sphingomonas Herbicidovorans MH,” Journal of Bacteriology, 1997, vol. 179, No…
[cited by applicant]
Nielsen T.K., et al., “Novel Insight into the Genetic Context of the cadAB Genes from a 4-Chloro-2-Methylphenoxyacetic Acid-Degrading Sphingomonas,” PLOS One, 2013, vol. 8, No. 12, e83346, pp. 1-9.
[cited by applicant]