IP Library › Granted Patent US 12,281,353
Granted Patent B1
US 12,281,353 · App. 17/173,065 · Granted Apr 22, 2025

Terephthalate biosensors and uses thereof

Inventors: Ramesh K. Jha (Los Alamos, NM); Taraka T. Dale (Los Alamos, NM); Ryan E. Bermel (Los Alamos, NM); Ellen Lee Neidle (Athens, GA)
Assignees: TRIAD NATIONAL SECURITY, LLC; UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
C12Q1/6825C07K14/4702C12Q1/6853G01N33/582
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Quick Facts
Patent No.
US 12,281,353
App. No.
17/173,065
Granted
Apr 22, 2025
Kind
B1
Abstract

Biosensors for detection terephthalic acid (TPA) and methods of their use are provided. The biosensors include a nucleic acid encoding a TphR protein, a promoter regulated by TPA, and a reporter operably linked to the promoter. Vectors and host cells including the biosensors are also provided.

Claims (13)

1. A terephthalic acid (TPA) biosensor comprising a nucleic acid encoding a TPA responsive transcription factor from Comamonas testosteroni (TphR) protein, a TphR regulated promoter comprising the nucleic acid sequence of nucleotides 4-114 of SEQ ID NO: 12, and a nucleic acid encoding a reporter protein operably linked to the promoter.

2. The TPA biosensor of claim 1 , wherein the reporter protein is a fluorescent protein.

3. The TPA biosensor of claim 2 , wherein the biosensor comprises a nucleic acid sequence with at least 95% sequence identity to nucleotides 2862-4457 of SEQ ID NO: 1 or the reverse complement thereof.

4. The TPA biosensor of claim 3 , wherein the biosensor comprises the nucleic acid sequence of nucleotides 2862-4457 of SEQ ID NO: 1 or the reverse complement thereof.

5. The TPA biosensor of claim 4 , wherein the biosensor consists of the nucleic acid sequence of nucleotides 2862-4457 of SEQ ID NO: 1 or the reverse complement thereof.

6. A vector or a host cell comprising the TPA biosensor of claim 1 .

7. The vector of claim 6 , wherein the vector comprises a nucleic acid sequence with at least 95% sequence identity to nucleotides 2862-4457 of SEQ ID NO: 1 or the reverse complement thereof.

8. The vector of claim 7 , wherein the vector comprises the nucleic acid sequence of nucleotides 2862-4457 of SEQ ID NO: 1 or the reverse complement thereof.

9. The vector of claim 6 , wherein the vector comprises the nucleic acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2.

10. The host cell of claim 6 , wherein the cell is a bacterial cell.

11. The host cell of claim 10 , wherein the bacterial cell is an Acinetobacter baylyi cell.

12. The host cell of claim 11 , wherein the Acinetobacter baylyi cell is an A. baylyi ADP1 cell.

13. A nucleic acid comprising the nucleic acid sequence of nucleotides 4-114 of SEQ ID NO: 12, encoding a terephthalic acid responsive transcription factor from Comamonas testosteroni (TphR) regulated promoter.

Assignments (4)
CORRECTIVE ASSIGNMENT TO CORRECT THE THE ASSIGNEE'S ADDRESS PREVIOUSLY RECORDED AT REEL: 063483 FRAME: 0531. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Jun 30, 2023
From: NEIDLE, ELLEN LEE
To: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
Reel/Frame 064185/0908 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 28, 2023
From: JHA, RAMESH K.; DALE, TARAKA T.; BERMEL, RYAN E.
To: TRIAD NATIONAL SECURITY, LLC
Reel/Frame 063483/0522 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 28, 2023
From: NEIDLE, ELLEN LEE
To: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
Reel/Frame 063483/0531 →
CONFIRMATORY LICENSE Recorded Apr 26, 2021
From: TRIAD NATIONAL SECURITY, LLC
To: U.S. DEPARTMENT OF ENERGY
Reel/Frame 056041/0391 →
Continuity (1)
Provisional Application 62972365 · Feb 10, 2020
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