Nucleic acids encoding polypeptides against IL-23
View Patent ↗The present invention relates to amino acid sequences that are directed against interleukin 23 (IL-23). The amino acid sequences of the present invention comprise two NANOBODY® molecules against IL-23 and one NANOBODY® molecule against serum albumin, linked by two linkers. In particular, the invention relates to the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 3 (listed in Table 1 and FIG. 1 ) (also referred to herein as “anti-IL 23 polypeptides of the invention”).
1. A nucleic acid that encodes an anti-interleukin 23 (IL-23) polypeptide comprising an amino acid sequence that is chosen from the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 3.
2. A nucleic acid that encodes an anti-interleukin 23 (IL-23) polypeptide comprising the amino acid sequence of SEQ ID NO: 2.
3. A nucleic acid that encodes an anti-interleukin 23 (IL-23) polypeptide comprising the amino acid sequence of SEQ ID NO: 3.
4. A non-human host or an isolated host cell that comprises the nucleic acid of claim 1 .
5. A non-human host or an isolated host cell that comprises the nucleic acid of claim 2 .
6. A non-human host or an isolated host cell that comprises the nucleic acid of claim 3 .
7. A method for producing an anti-IL-23 polypeptide, wherein said method comprises
expressing the anti-IL-23 polypeptide encoded by the nucleic acid of claim 1 in a non-human host or an isolated host cell.
8. The method of claim 7 , further comprising isolating and/or purifying the anti-IL-23 polypeptide.
9. A method for producing an anti-IL-23 polypeptide, wherein said method comprises cultivating the
non-human host or isolated host cell of claim 4 under conditions that are such that said non-human host or isolated host cell expresses the anti-IL-23 polypeptide encoded by the nucleic acid.
10. The method of claim 9 , further comprising isolating and/or purifying the anti-IL-23 polypeptide.
11. A method for producing an anti-IL-23 polypeptide, wherein said method comprises maintaining the non-human host or isolated host cell of claim 4 under conditions that are such that said non-human host or isolated host cell expresses the anti-IL-23 polypeptide encoded by the nucleic acid.
12. The method of claim 11 , further comprising isolating and/or purifying the anti-IL-23 polypeptide.