Rotors for immunoassays
The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.
1. A device comprising a sample port and a surface, wherein the surface comprises a single channel in fluid communication with the sample port and a circular flow path at a peripheral edge of the surface, wherein the sample port and/or the channel comprises a conjugate comprising a binding partner that can specifically bind to an analyte, and wherein the circular flow path comprises an immobilized capture ligand capable of binding an analyte-conjugate complex.
2. The device of claim 1 , wherein spinning the surface and the sample port about a fixed axis generates a centrifugal force that causes a sample deposited in the sample port to flow through the channel and come into fluid contact with the capture ligand.
3. The device of claim 1 , wherein the sample port and/or channel comprises a plurality of conjugates.
4. The device of claim 1 , wherein the circular flow path comprises a plurality of capture ligands.
5. The device of claim 1 , wherein the binding partner is conjugated to a detectable entity.
6. The device of claim 5 , wherein the detectable entity is a metallic nanoparticle or metallic nanoshell.
7. The device of claim 5 , wherein the detectable entity is selected from the group consisting of gold nanoparticles, silver nanoparticles, copper nanoparticles, platinum nanoparticles, cadmium nanoparticles, composite nanoparticles, gold hollow spheres, gold-coated silica nanoshells, and silica-coated gold shells.
8. The device of claim 5 , wherein the detectable entity is an enzyme.
9. The device of claim 8 , wherein the enzyme is selected from the group consisting of alkaline phosphatase, horseradish peroxidase, beta-galactosidase, beta-lactamase, luciferase, myeloperoxidase, and amylase.
10. The device of claim 1 , wherein the surface further comprises at least one absorbing entity located downstream from the capture ligand.
11. The device of claim 3 , wherein the plurality of conjugates comprises at least a first conjugate capable of binding a first analyte and a second conjugate capable of binding a second analyte, wherein the first and second analytes are different.
12. The device of claim 3 , wherein each conjugate in the plurality of conjugates is capable of binding a different analyte.
13. The device of claim 3 , wherein the plurality of conjugates comprise binding partners conjugated to different detectable entities.
14. The device of claim 3 , wherein each of said plurality of immobilized capture ligands specifically binds to a different analyte-conjugate complex.
15. The device of claim 3 , wherein each of said plurality of immobilized capture ligands are capable of specifically binding to the same analyte-conjugate complex.
16. The device of claim 15 , wherein the immobilized capture ligands are immobilized at different concentrations.
17. The device of claim 4 , wherein the plurality of capture ligands are arranged sequentially along the circular path.
18. The device of claim 1 , wherein the sample port comprises a blood separator material that allows plasma from a blood sample to pass into the channel, while retaining cellular material from the blood sample in the sample port.
19. A system for detecting target analytes in a sample, comprising the device of claim 1 .