Printer-finisher system for data storage in DNA
Provided herein are systems and methods for storing digital information by assembling an identifier nucleic acid molecule from at least a first component nucleic acid molecule and a second component nucleic acid molecule. The system may include a first printhead configured to dispense a first droplet of a first solution comprising the first component nucleic acid molecule onto a coordinate on a substrate, and a second printhead configured to dispense a second droplet of a second solution comprising the second component nucleic acid molecule onto the coordinate on the substrate, such that the first and second component nucleic acid molecules are collocated on the substrate. The system may include a finisher that dispenses a reaction mix onto the coordinate on the substrate to physically link the first and second component nucleic acid molecules, provides a condition necessary to physically link the first and second component nucleic acid molecules, or both.
1. A system for assembling an identifier nucleic acid molecule, the system comprising:
(a) a first printhead configured to dispense a first droplet of a first solution comprising a first component nucleic acid molecule of the identifier nucleic acid molecule onto a coordinate on a substrate;
(b) a second printhead configured to dispense a second droplet of a second solution comprising a second component nucleic acid molecule of the identifier nucleic acid molecule onto the coordinate on the substrate, such that the first and second component nucleic acid molecules are collocated on the substrate; and
(c) a finisher that dispenses a reaction mix onto the coordinate on the substrate to physically link the first and second component nucleic acid molecules, provides a condition necessary to physically link the first and second component nucleic acid molecules, or both;
the identifier nucleic acid molecule being configured to represent a position and a value of a symbol in a string of symbols.
2. The system of claim 1 , wherein the finisher comprises a third printhead configured to dispense the reaction mix onto the coordinate on the substrate.
3. The system of claim 1 , wherein the finisher dispenses the reaction mix onto the coordinate before the first printhead dispenses the first droplet onto the coordinate, before the second printhead dispenses the second droplet onto the coordinate, or both.
4. The system of claim 1 , wherein the first or second solutions incorporate a dye, the system further comprising a spot imager including a camera that verifies a proper dispense of the first and second droplets.
5. The system of claim 1 , wherein the first and second printheads are mounted within the system at an angle relative to motion of the substrate, wherein the angle enables overprinting on the coordinate.
6. The system of claim 1 , wherein at least one of the first printhead or the second printhead is a MEMS thin film piezo ink jet head or a MEMS thermal ink jet head.
7. The system of claim 1 , wherein at least one of the first solution, the second solution, or the reaction mix comprises an additive or a ligase.
8. The system of claim 7 , wherein the additive comprises at least one of a humectant, a surfactant, or a biocide.