Systems and methods for mounting biosensors using a consumable fluid reservoir
A system and method for controlled fluid handling and processing of singulated biosensors. The system includes a consumable for mounting and protecting biosensors.
1. A system for mounting biosensors in a protective carrier:
the system comprising at least one consumable, the at least one consumable comprising:
a cap;
a biosensor;
a bonding element;
a base; and,
a strip configured to hold at least a portion of the at least one consumable, and,
a well plate configured to hold at least one strip.
2. The system of claim 1 , wherein the base comprises at least two levels, and wherein a first level of the base is configured to mount a biosensor.
3. The system of claim 1 , wherein the cap defines at least one internal volume; the at least one internal volume defined by a cap sidewall and the base.
4. The system of claim 3 , wherein the at least one internal volume has a divider, wherein the divider divides the internal volume into a fluidic access port and a fluid reservoir.
5. The system of claim 3 , wherein the at least one internal volume is in fluid communication with an incubation chamber.
6. The system of claim 1 , wherein a bottom level of the base is configured to be accepted by a strip in a friction fit.
7. The system of claim 1 , wherein the strip is configured to be accepted by a well plate in a friction fit.
8. The system of claim 1 , wherein the biosensor is an arrayed imaging reflectometry (AIR) sensor chip for small or large molecule detection.
9. The system of claim 1 , wherein a portion of a cap sidewall is configured to be engaged by a robotic arm.
10. The system of claim 1 , wherein the bonding element is configured to bond the biosensor to the top surface to the base.
11. The system of claim 1 , wherein the cap is configured to fit on top of the base to form a biosensor incubation volume between the base and the cap.
12. The system of claim 11 , wherein the biosensor incubation volume can be filled with a fluid.
13. The system of claim 1 , wherein the base further comprises at least one channel configured to allow a tool to access a bottom surface of the biosensor.
14. The system of claim 1 , wherein the consumable further comprises a cap seal.
15. The system of claim 1 , wherein a top portion of the consumable is further configured to trap light, scatter light, or reduce optical noise.
16. A system for mounting biosensors in a protective carrier, the system comprising at least one consumable, the at least one consumable comprising:
a cap having a bottom level, a middle level, and a top level;
a biosensor;
a bonding element;
a base having a bottom level and a top level;
wherein the top level of the cap has an internal volume defined by a cap sidewall and the base, wherein the internal volume is divided into a fluidic access port and a fluid reservoir;
wherein the middle level of the cap has an incubation chamber configured to house the biosensor;
wherein the bottom level of the cap has a bottom cap volume configured to accept the top level of the base;
a strip configured to hold at least one consumable; and
a well plate configured to hold at least one strip.
17. A method of mounting a biosensor in at least one consumable, the method comprising:
a. providing the at least one consumable, the at least one consumable comprising:
a cap having a bottom level, a middle level, and a top level;
a biosensor;
a bonding element;
a base having a bottom level and a top level;
wherein the top level of the cap has an internal volume defined by a cap sidewall and the base, wherein the internal volume is divided into a fluidic access port and a fluid reservoir;
wherein the middle level of the cap has an incubation chamber configured to house the biosensor;
wherein the bottom level of the cap has a bottom cap volume configured to accept the top level of the base; and,
b. providing at least one strip configured to hold at least one consumable;
c. providing a well plate configured to hold at least one strip;
d. placing the biosensor on the top surface of the bonding element;
e. placing the cap on the base;
f. placing the base in the at least one strip; and
g. adding fluid to the fluidic access port or the fluid reservoir.
18. The method of claim 17 , wherein the well plate is placed in placed in a bioassay machine.
19. The method of claim 17 , wherein the consumable is placed in the well plate without the strip.
20. The method of claim 17 , wherein the bonding element is placed on the base before the biosensor is placed on the base.