Apparatuses and methods for suspending and washing the contents of a plurality of cuvettes

1. A method of washing a plurality of cuvettes each containing a fluid sample, the method comprising:

adding magnetic particles to the plurality of cuvettes, the plurality of cuvettes located in a rotor configured to rotate the plurality of cuvettes about a vertical axis;

rotating, via the rotor, the plurality of cuvettes over a traveler mechanism, the traveler mechanism located beneath the rotor, the traveler mechanism including:

a guide rail provided in a vertical orientation with respect to the rotor,

a sliding device configured to vertically translate along the guide rail, and

a magnet holder connected to the sliding device, the magnet holder configured to retain a plurality of magnets that are spaced apart at a distance to enable a respective cuvette to pass between adjacent magnets,

wherein the sliding device is configured to translate vertically toward the rotor;

raising the plurality of magnets via the traveler mechanism so that each cuvette of the plurality of cuvettes has a first magnet of the plurality of magnets adjacent to a first side and a second magnet of the plurality of magnets adjacent to an opposite second side;

causing the magnetic particles to be suspended in the plurality of cuvettes with the plurality of magnets;

lowering a plurality of probes so that each cuvette of the plurality of cuvettes has a probe of the plurality of probes located therein; and

aspirating fluid from each cuvette of the plurality of cuvettes with a respective probe.

2. The method of claim 1 , wherein the plurality of probes are included within a wash mechanism that is located above the rotor, the wash mechanism including:

an arm configured to be movable vertically and horizontally with respect to the rotor to enable lowering the plurality of probes;

a plurality of tubes supported by the arm and connected to at least one fluid supply; and

a base connected to an end of the arm, the base configured to retain the plurality of probes that are each fluidly connected to one of the plurality of tubes to enable aspirating the fluid from each cuvette of the plurality of cuvettes.

3. The method of claim 2 , wherein aspirating the fluid from each cuvette of the plurality of cuvettes includes causing the plurality of probes, via the plurality of tubes, to wash the fluid sample within each of the plurality of cuvettes by at least one of injecting fluid into or aspirating fluid from the plurality of cuvettes when the arm is horizontally aligned with the plurality of cuvettes and translated vertically to place at least a portion of the plurality of probes respectively into the plurality of cuvettes.

4. The method of claim 3 , wherein the plurality of probes is lowered while the plurality of magnets suspend the magnetic particles and the bound fluid sample located within each of the plurality of cuvettes.

5. The method of claim 3 , wherein aspirating the fluid is performed using a first section of each respective probe and injecting the fluid is performed using a second section of each respective probe.

6. The method of claim 3 , further comprising heating at least one of the injected fluid or the aspirated fluid via a heater in proximity to the plurality of tubes.

7. The method of claim 1 , further comprising translating vertically the sliding device away from the rotor after the suspension of the magnetic particles is no longer needed.

8. The method of claim 1 , wherein raising the magnets includes positioning a magnet having a first polarity adjacent to the first side and a magnet having an opposite second polarity adjacent to the second side.

9. The method of claim 1 , further comprising raising the plurality of probes away from the cuvettes after aspirating the fluid.

10. The method of claim 1 , further comprising detecting, via a probe positioning sensor, if one or more of the probes becomes misaligned when lowering the plurality of probes.

11. The method of claim 1 , further comprising causing the plurality of cuvettes to be locked into the rotor using a removable lid.

12. The method of claim 1 , further comprising raising the plurality of magnets and lowering the plurality of probes in directions parallel to the vertical axis.

13. The method of claim 1 , further comprising causing a locking mechanism of the traveler mechanism to engage a protrusion located on an underside of the rotor to hold the traveler mechanism in place when the plurality of cuvettes is located adjacent to the plurality of magnets.

14. The method of claim 1 , wherein the fluid includes a wash buffer and the fluid sample includes at least one of a reagent, a conjugate sample, or luminescent label.

15. A method of washing a plurality of cuvettes each containing a fluid sample, the method comprising:

adding magnetic particles to the plurality of cuvettes, the plurality of cuvettes located in a rotor configured to rotate the plurality of cuvettes about a vertical axis;

rotating, via the rotor, the plurality of cuvettes over a traveler mechanism, the traveler mechanism located beneath the rotor, the traveler mechanism including:

a guide rail provided in a vertical orientation with respect to the rotor,

a sliding device configured to vertically translate along the guide rail, and

a magnet holder connected to the sliding device, the magnet holder configured to retain a plurality of magnets that are spaced apart at a distance to enable a respective cuvette to pass between adjacent magnets,

wherein the sliding device is configured to translate vertically toward the rotor;

raising the plurality of magnets via the traveler mechanism so that each cuvette of the plurality of cuvettes has a first magnet of the plurality of magnets adjacent to a first side and a second magnet of the plurality of magnets adjacent to an opposite second side; and

causing the magnetic particles to be suspended in the plurality of cuvettes with the plurality of magnets for washing.

16. The method of claim 15 , wherein washing includes:

lowering a plurality of probes so that each cuvette of the plurality of cuvettes has a probe of the plurality of probes located therein; and

at least one of injecting fluid into or aspirating fluid from the plurality of cuvettes via the plurality of probes.

17. The method of claim 16 , wherein the plurality of probes is lowered via a wash mechanism that is located above the rotor, the wash mechanism including:

an arm configured to be movable vertically and horizontally with respect to the rotor to enable lowering the plurality of probes;

a plurality of tubes supported by the arm and connected to at least one fluid supply; and

a base connected to an end of the arm, the base configured to retain the plurality of probes that are each fluidly connected to one of the plurality of tubes.

18. The method of claim 17 , wherein the washing occurs after the arm is horizontally aligned with the plurality of cuvettes and translated vertically to place at least a portion of the plurality of probes respectively into the plurality of cuvettes.

19. The method of claim 16 , wherein the plurality of probes is lowered while the plurality of magnets suspend the magnetic particles and the bound fluid sample located within each of the plurality of cuvettes, and

wherein aspirating the fluid is performed using a first section of each respective probe and injecting the fluid is performed using a second section of each respective probe.

20. The method of claim 15 , wherein the fluid includes a wash buffer and the fluid sample includes at least one of a reagent, a conjugate sample, or luminescent label.