Salvianolic acid-gelatin conjugate hydrogel particles
The present disclosure relates to salvianolic acid-gelatin conjugate load retentive hydrogel nanoparticles useful for oral delivery of salvianolic acid, pharmaceutical compositions comprising the same, and methods of use and preparation thereof.
1. A method of preparing a nanoparticle comprising: a core and a polymer shell coating the core, wherein the polymer shell comprises an inner shell comprising at least one polysaccharide and optionally an outer shell comprising chitosan, shellac, or an enteric coating material, wherein the core comprises a salvianolic acid B-gelatin conjugate (SAB-gelatin conjugate) comprising gelatin covalently bonded to salvianolic acid B (SAB), the method comprising:
contacting gelatin, SAB, a coupling agent, and optionally a coupling additive thereby forming the SAB-gelatin conjugate; coating the SAB-gelatin conjugate with the at least one polysaccharide thereby forming an inner shell coated gelatin conjugate; and optionally coating the inner shell coated gelatin conjugate with chitosan, shellac, or an enteric coating material thereby forming the nanoparticle, wherein the SAB-gelatin conjugate is represented by Formula 1:
or a pharmaceutically acceptable salt thereof, wherein
R 1 and R 2 are independently selected from the group consisting of OH and —NHR 3 ; and
R 3 for each instance is independently an amine containing amino acid side chain of the gelatin or a N-terminal amine of the gelatin, with the proviso that at least one of R 1 or R 2 is —NHR 3 ;
the at least one polysaccharide is selected from the group consisting of alginate, agarose, hyaluronic acid, carboxymethyl cellulose, hydroxypropyl methylcellulose, and combinations thereof; and
the enteric coating material is selected from the group consisting of an anionic copolymer derived from methacrylic acid and ethyl acrylate, hydroxypropyl methylcellulose phthalate, polyvinyl acetate phthalate, polyvinyl acetate phthalate, cellulose acetate phthalate, or a combination thereof.
2. The method of claim 1 , wherein the coupling agent is 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC); and the coupling additive is N-hydroxysuccinimide (NHS).
3. The method of claim 1 , wherein the gelatin and the SAB are present in a mass ratio between 3:1 to 1:10, respectively.
4. The method of claim 2 , wherein the EDC, gelatin and the SAB are present in a mass ratio between 4:3:1 to 40:1:10, respectively.
5. The method of claim 1 , wherein the at least one polysaccharide is selected from the group consisting of alginate, agarose, or a combination thereof; and the SAB-gelatin conjugate and the at least one polysaccharide are present in a mass ratio of 1:4 to 99:1, respectively.
6. The method of claim 1 , wherein the inner shell coated gelatin conjugate and the chitosan, shellac, or an enteric coating material are present in a mass ratio of 1:4 to 99:1, respectively.
7. The method of claim 4 , wherein the at least one polysaccharide is selected from the group consisting of alginate, agarose, or a combination thereof; and the SAB-gelatin conjugate and the at least one polysaccharide are present in a mass ratio of 7:3 to 4:1, respectively.
8. The method of claim 7 , wherein the inner shell coated gelatin conjugate and the chitosan or shellac are present in a mass ratio of 7:3 to 4:1, respectively.