Granted Patent
B2
US 12,222,346 · App. 17/218,365 · Granted Feb 11, 2025
Stable nanopores and nanopore arrays for ionic and other measurements
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Abstract
Provided are nanopore devices that include a hafnium oxide coating on the pores of the devices. Such devices exhibit improved stability, especially when in a salt solution environment. Also provided are related methods.
Claims (36)
1. An etch-resistant nanopore component, comprising:
a first membrane,
the membrane having a pore extending therethrough,
the pore having an inner wall defined by the membrane; and
a coating of hafnium oxide conforming to and surmounting at least a portion of the inner wall, and
the pore having, inclusive of the coating of hafnium oxide, a narrowest cross-sectional dimension of from about 1 nm to about 100 nm.
2. The component of claim 1 , wherein the membrane comprises silicon nitride.
3. The component of claim 1 , wherein the coating of hafnium oxide defines a thickness, at a location along the coating, in the range of from about 0.1 nm to about 2 nm.
4. The component of claim 1 , wherein the pore is characterized as an hourglass in configuration.
5. The component of claim 1 , wherein the pore is characterized as essentially cylindrical in configuration.
6. The component of claim 1 , wherein the membrane comprises a thinned region through which the pore extends.
7. The component of claim 1 , wherein the membrane comprises a plurality of pores extended therethrough, each of the plurality of pores defining an edge and an inner wall, and each of the edges and inner walls being surmounted by the coating of hafnium oxide.
8. The component of claim 1 , wherein the membrane defines a thickness of from about 1 nm to about 100 nm.
9. The component of claim 1 , wherein the narrowest cross-sectional dimension of the pore remains essentially constant upon exposure to 1 M KCl for about 6 hours.
10. The component of claim 1 , wherein the membrane is supported by a silicon oxide layer.
11. A method, comprising: translocating a macromolecule through the pore of a component according to claim 1 .
12. The method of claim 11 , further comprising applying a gradient to effect the translocation.
13. The method of claim 11 , further comprising collecting one or more signals evolved during the translocating.
14. The method of claim 13 , further comprising correlating the one or more signals to one or more structural characteristics of the macromolecule.
15. The method of claim 11 , wherein the macromolecule is an oligonucleotide or a polynucleotide.
16. The component of claim 1 , further comprising:
a second membrane defining a thickness,
the second membrane having a pore extending there through,
the pore of the second membrane defining an edge and defining an inner wall, and
the first membrane and the second membrane being arranged such a line can be drawn so as to extend through the thickness of the first membrane and the thickness of the second membrane.
17. The component of claim 16 , wherein the second membrane comprises a thinned region through which the pore of the second membrane extends.
18. The component of claim 16 , wherein the pore of the first membrane is at least partially in register with the pore of the second membrane.
19. The component of claim 16 , wherein the first membrane defines a plurality of pores formed therein.
20. The component of claim 19 , wherein at least one pore of the first membrane is at least partially in register with the pore of the second membrane.
21. The component of claim 16 , further comprising a coating of hafnium oxide surmounting at least a portion of the edge and the inner wall of the pore of the second membrane.
22. A method, comprising:
forming a pore extending through a membrane,
the pore having an edge and having an inner wall defined by the membrane; and
applying a hafnium oxide coating so as to conform to and surmount at least a portion of the inner wall,
the pore having, inclusive of the coating of hafnium oxide, a narrowest cross-sectional dimension of from about 1 nm to about 100 nm.
23. The method of claim 22 , further comprising forming a thinned region in the membrane and forming the pore such that the pore extends through the thinned region of the membrane.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 1, 2021
From: DRNDIC, MARIJA; CHOU, YUNG-CHIEN
To: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
Reel/Frame 056397/0286 →
Continuity (2)
Provisional Application
63003434
· Apr 1, 2020
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