Stable inoculant compositions and methods for producing same
[A]queous inoculant compositions comprising microbial spores and methods for enhancing the survival and/or stability of microbial spores in an inoculant composition. In some embodiments, the aqueous inoculant compositions comprise microbial spores, one or more dispersants, one or more protectants, one or more aqueous additives in a non-aqueous liquid carrier.
1. An aqueous inoculant composition, comprising:
microbial spores comprising about 0.1 to about 15% by weight of said inoculant composition;
one or more protectants comprising about 0.5 to about 5% by weight of said composition, said one or more protectants comprising one or more sugars and/or one or more sugar alcohols;
one or more dispersants comprising about 0.1 to about 5% by weight of said composition;
one or more aqueous additives comprising about 0.01 to about 5% by weight of said composition; and
one or more polyethylene glycols comprising about 75 to about 95% by weight of said inoculant composition.
2. The inoculant composition of claim 1 , wherein said one or more protectants comprises about 1 to about 2% by weight of said composition.
3. The inoculant composition of claim 1 , wherein said one or more protectants comprises one or more disaccharides and one or more sugar alcohols.
4. The inoculant composition of claim 1 , wherein said one or more protectants comprises maltose, sucrose and/or trehalose.
5. The inoculant composition of claim 1 , wherein said one or more protectants comprises arabitol, mannitol, sorbitol and/or xylitol.
6. The inoculant composition of claim 1 , wherein said one or more dispersants comprises about 1 to about 4% by weight of said composition.
7. The inoculant composition of claim 1 , wherein said one or more dispersants comprises one or more non-ionic surfactants and/or one or more wetting agents.
8. The inoculant composition of claim 1 , wherein said one or more dispersants comprises one or more alcohol ethoxylates.
9. The inoculant composition of claim 1 , wherein said one or more dispersants comprises one or more naphthalene sulfonates.
10. The inoculant composition of claim 1 , wherein said one or more aqueous additives comprises about 0.5 to about 2.5% by weight of said composition.
11. The inoculant composition of claim 1 , wherein water comprises at least 0.75% by weight of said composition.
12. The inoculant composition of claim 1 , wherein said one or more polyethylene glycols comprises about 80 to about 90% by weight of said inoculant composition.
13. The inoculant composition of claim 1 , wherein said one or more polyethylene glycols comprises PEG 200, PEG 300 and/or PEG 400.
14. The inoculant composition of claim 1 , wherein said inoculant composition further comprises one or more pesticides.
15. The inoculant composition of claim 1 , wherein said inoculant composition further comprises one or more lipo-chitooligosaccharides, one or more chitin oligomers and/or one or more flavonoids.
16. The inoculant composition of claim 1 , wherein said inoculant composition further comprises one or more drying agents.
17. A plant propagation material at least partially coated on the outer surface with a coating comprising the inoculant composition of claim 1 .
18. A kit, comprising:
the coated plant propagation material of claim 17 ; and
a container housing said coated plant propagation material.
19. A method, comprising applying the inoculation composition of claim 1 to a plant propagation material, optionally a seed.
20. A method, comprising planting the coated plant propagation material of claim 17 in a growth medium, optionally a soil.