IP Library › Granted Patent US 12,338,442
Granted Patent B2
US 12,338,442 · App. 17/228,994 · Granted Jun 24, 2025

Aptamers against clostridium difficile

Inventors: Ronald J. Shannon (Ardmore, PA); Michael McIntyre (Ardmore, PA); David Bunka (York, GB); Edward Barnes (York, GB)
Assignee: LIV PROCESS, INC.
C12N15/115C12Q1/689C12N2310/16
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,338,442
App. No.
17/228,994
Filed
Apr 13, 2021
Granted
Jun 24, 2025
Kind
B2
Examiner
SHIN, DANA H
Art Unit
1635
USPC
435/6.15
Abstract

Compositions comprising aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and/or isolating Clostridium difficile spores is provided.

Claims (15)

1. An aptamer having a specific binding affinity for a surface protein of Clostridium difficile spore, wherein the aptamer comprises from position 22 to position 61 of the nucleotide sequence of SEQ ID NO: 14.

2. The aptamer of claim 1 , wherein the surface protein is exosporium layer protein CdeM.

3. The aptamer of claim 2 , wherein the surface protein is CdeM having an amino acid sequence as set forth in SEQ ID NO: 19.

4. The aptamer of claim 1 , wherein the aptamer comprises a detectable label.

5. The aptamer of claim 4 , wherein the detectable label comprises a fluorophore, a nanoparticle, a radioactive isotope, a biotin, a protein or a combination thereof.

6. An apparatus for detecting the presence, absence or level of Clostridium difficile spores in a sample, the apparatus comprising:

a support; and

at least one aptamer having a specific binding affinity for a surface protein of Clostridium difficile spore, wherein the at least one aptamer comprises from position 22 to position 61 of the nucleotide sequence of SEQ ID NO: 14.

7. The apparatus of claim 6 , wherein the surface protein is exosporium layer protein CdeM.

8. A composition comprising at least one aptamer having a specific binding affinity for a surface protein of Clostridium difficile spore, wherein the at least one aptamer comprises from position 22 to position 61 of the nucleotide sequence of SEQ ID NO: 14.

9. The composition of claim 8 , wherein the surface protein is exosporium layer protein CdeM.

10. The composition of claim 8 , wherein at least one aptamer comprises a detectable label.

11. The aptamer of claim 4 , wherein the aptamer is conjugated to a fluorophore.

12. The aptamer of claim 5 , wherein the protein is an enzyme or an antibody.

13. The aptamer of claim 5 , wherein the nanoparticle is a quantum dot, a colloidal metallic particle, a colloidal non-metallic particle, an organic polymer, a nanofiber, or a nanotube.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 3, 2021
From: BIOVECTOR, INC.
To: LIV PROCESS, INC.
Reel/Frame 058796/0180 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 9, 2021
From: SHANNON, RONALD J.; MCINTYRE, MICHAEL; BUNKA, DAVID; BARNES, EDWARD
To: BIOVECTOR, INC.
Reel/Frame 057123/0438 →
Continuity (4)
Division 16893935 · Jun 5, 2020
Provisional Application 62983095 · Feb 28, 2020
Provisional Application 62857639 · Jun 5, 2019
Related Publication 20210332362A1 · Oct 28, 2021
References Cited (64)
US 8592202B2 · Heyduk et al. · 2013 [cited by applicant]
US 8697374B2 · Rajagopal et al. · 2014 [cited by applicant]
US 8895241B2 · Ochsner et al. · 2014 [cited by applicant]
US 9081010B2 · Ochsner et al. · 2015 [cited by applicant]
US 10145844B2 · Cameron et al. · 2018 [cited by applicant]
US 11001847B2 · Shannon · 2021 [cited by applicant]
US 11104905B2 · Shannon · 2021 [cited by applicant]
US 11898146B2 · Shannon et al. · 2024 [cited by applicant]
US 20040023266A1 · Vivekananda · 2004 [cited by applicant]
US 20080020939A1 · Stanton et al. · 2008 [cited by applicant]
US 20090304683A1 · Dimitrov et al. · 2009 [cited by applicant]
US 20100291100A1 · Macinga · 2010 [cited by applicant]
US 20110287557A1 · Zhang et al. · 2011 [cited by applicant]
US 20120231467A1 · Ochsner et al. · 2012 [cited by applicant]
US 20120308569A1 · Chan et al. · 2012 [cited by applicant]
US 20140230087A1 · Hartig et al. · 2014 [cited by applicant]
US 20150056627A1 · Karkkainen et al. · 2015 [cited by applicant]
US 20150346199A1 · Li et al. · 2015 [cited by applicant]
US 20160003835A1 · Halbert · 2016 [cited by examiner]
US 20160143274A1 · Bingham et al. · 2016 [cited by applicant]
US 20160330971A1 · Joseph · 2016 [cited by applicant]
US 20170362307A1 · Ingber et al. · 2017 [cited by applicant]
US 20180003712A1 · Haam et al. · 2018 [cited by applicant]
US 20180271423A1 · Agarwal et al. · 2018 [cited by applicant]
US 20190069836A1 · Hettrick · 2019 [cited by applicant]
US 20190071714A1 · Li et al. · 2019 [cited by applicant]
US 20200332296A1 · Kang · 2020 [cited by applicant]
US 20200385730A1 · Shannon et al. · 2020 [cited by applicant]
US 20200385731A1 · Shannon et al. · 2020 [cited by applicant]
US 20220177886A1 · Shannon et al. · 2022 [cited by applicant]
US 20220307029A1 · Shannon et al. · 2022 [cited by applicant]
US 20230200397A1 · Shannon et al. · 2023 [cited by applicant]
US 20230228751A1 · Tolley et al. · 2023 [cited by applicant]
CN 109517823A · 2019 [cited by applicant]
CN 110938632A · 2020 [cited by applicant]
CN 111849994A · 2020 [cited by applicant]
CN 112557349A · 2021 [cited by applicant]
GB 2491117A · 2012 [cited by applicant]
WO 2004058146A2 · 2004 [cited by applicant]
WO 2010126670A2 · 2010 [cited by applicant]
WO 2014169344A1 · 2014 [cited by applicant]
WO 2016073562A1 · 2016 [cited by applicant]
WO 2018106945A1 · 2018 [cited by applicant]
WO 2020247755A1 · 2020 [cited by applicant]
WO 2021202440A1 · 2021 [cited by applicant]
WO 2021211921A2 · 2021 [cited by applicant]
WO 2022120004A1 · 2022 [cited by applicant]
Wang et al., Screening of DNA aptamers against myoglobin using a positive and negative selection units integrated microfluidic chip and its biosensing application, Analytical Chemistry, vol. 86, pp. 6572-6579. (Year: 20… [cited by examiner]
Ikanovic et al., “Fluorescence Assay Based on Aptamer-Quantum Dot Binding to Bacillus thuringiensis Spores,” Journal of Fluorescence, Jan. 31, 2007, vol. 17, pp. 193-199. [cited by applicant]
International Search Report issued in corresponding International Patent Application No. PCT/US20/36333, mailed Nov. 19, 2020. [cited by applicant]
Calderon-Romero et al., “Clostridium difficile exosporium cysteine-rich proteins are essential for the morphogenesis of the exosporium layer, spore resistance, and affect C. difficile pathogenesis,” PLOS Pathogens, Aug.… [cited by applicant]
Chan et al., “Antisense Oligonucleotides: From Design to Therapeutic Application,” Clinical and Experimental Pharmacology Physiology, 2006, vol. 33, No. 5-6, pp. 533-540. [cited by applicant]
Diaz-Gonzalez et al., “Protein Composition of the Outermost Exosporium-like Layer of Clostridium difficile 630 Spores,” Journal of Proteomics, Jun. 18, 2015, vol. 123, pp. 1-13. [cited by applicant]
Hong et al., “The Spore Coat Protein CotE Facilitates Host Colonization by Clostridium Difficile,” The Journal of Infectious Diseases, Dec. 12, 2017, vol. 216, No. 11, pp. 1452-1459. [cited by applicant]
Jang, Sung Key, “A ‘15-minute’ quick diagnostic testing for newly emerging viruses introduced.” Pohang University of Science and Technology, pp. 1-3, Mar. 23, 2020. [cited by applicant]
Johansson “Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers,” Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols, 2006, No. pp. 17-29. [cited by applicant]
Marras et al., “Efficiencies of Fluorescence Resonance Energy Transfer and Contact-mediated Quenching in Oligonucleotide Probes,” Nucleic Acids Research, Nov. 1, 2002, vol. 30, No. 21, p. e122. [cited by applicant]
Liu et al., “In Vitro Selection of Circular DNA Aptamers for Biosensing Applications,” Angewandte Chemie International Edition, 2019, vol. 58, pp. 8013-8017. [cited by applicant]
Omidbakhsh, “Evaluation of Sporicidal Activities of Selected Environmental Surface Disinfectants: Carrier Tests with the Sores of Clostridium Difficile and Its Surrogates”, American Journal of Infection Control, vol. 38… [cited by applicant]
Hypothetical protein CD630_ 16130 [Clostridioides difficile 630], record removed, Old YP 001088114.1, accessed and retrieved from ncbi.nlm.nih.gov on Nov. 6, 2023. (Year: 2023). [cited by applicant]
CotA family spore coat protein [Clostridioides difficile], NCBI Reference Sequence: WP 003436448.1, accessed and retrieved from ncbi.nlm.nih.gov on Nov. 6, 2023. (Year: 2023). [cited by applicant]
Katilius et al., “Exploring the Sequence Space of a dna Aptamer Using Microarrays”, Nucleic Acids Res., vol. 35, No. 22, pp. 7626-7635, 2007. [cited by applicant]
Mascini et al., “Nucleic Acid and Peptide Aptamers: Fundamentals and Bioanalytical Aspects”, Angew Chem. Int. Ed. Engl., vol. 51, No. 6, pp. 1316-1332, Feb. 6, 2012. [cited by applicant]
Tkhawkho et al., “Destruction of Clostridium Difficile Spores Colitis Using Acidic Electrolyzed Water”, Am. J. Infect. Control, vol. 45, No. 9, pp. 1053, Sep. 1, 2017. [cited by applicant]
Cited By (1)
US 12,708,119