Granted Patent B2

US 12,186,410 · App. 17/234,323 · Granted Jan 7, 2025

Inventors: Jesus Manuel Perez (Orlando, FL); Santimukul Santra (Orlando, FL)

Assignee: University of Central Florida Research Foundation, Inc.

A61K51/065A61K31/519A61K49/0423A61P35/00C08G63/6882C08G83/002

Abstract

Claims (18)

1. A method of identifying a solid tumor cell target, comprising,

1) contacting a cell with an effective amount of a composition comprising at least one polymeric nanoparticle conjugated with a targeting ligand that is a substrate for a solid tumor-specific cell protein, wherein the nanoparticle further comprises an imaging compound and an anti-cancer agent; wherein the polymeric nanoparticle comprises a hyperbranched polyester and/or polyamide polymer, wherein the hyperbranched polyester and/or polyamide polymer has the formula:

wherein A is a heteroatom independently selected from nitrogen and oxygen; R 1 and R 2 are independently selected from hydrogen atom, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; 1 is an integer from 1 to 5; m is 0, 1, or 2; n is an integer from 1 to 5; and o is an integer from 2 or greater;

2) identifying one or more nanoparticles bound to the cells by using imaging devices; and optionally,

3) monitoring the solid tumor cell target by repeating 1) and 2).

2. The method of claim 1 , further comprising treating the solid tumor cell by killing or inhibiting its growth.

3. The method of claim 1 , wherein the solid tumor cell is a prostate cancer cell, a breast cancer cell, a colon cancer cell, a pancreas cancer cell, or a lung cancer cell.

4. The method of claim 1 , wherein the nanoparticle further comprises, in its hydrophobic interior, a therapeutic agent.

5. The method of claim 1 , wherein the polymeric nanoparticle is biodegradable.

6. The method of claim 1 , wherein the polymeric nanoparticle has one or more internal hydrophobic pockets and a hydrophilic outer surface.

7. The method of claim 1 , wherein the polymeric nanoparticle further comprises a hydrophobic near-infrared fluorescent dye encapsulated therein.

8. The method of claim 4 , further comprising a fluorescent dye co-encapsulated with said therapeutic agent.

9. The method of claim 1 , wherein the polymeric nanoparticle further comprises one or more imaging compounds encapsulated therein.

10. The method of claim 1 , wherein the polymeric nanoparticle further comprises a X-ray, MRI, or PET detectable compound.

11. The method of claim 1 , wherein the polymeric nanoparticle further comprises a metal compound comprising Au, Ag, Pd, Pt, Cu, Ni, Co, Fe, Mn, Ru, Rh, Os, or Ir.

12. The method of claim 1 , wherein the polymeric nanoparticle further comprises a radionuclide comprising 90 Y, 177 Lu, 18 F, 64 Cu, 67 Cu, 89 Zr, 111 In, 124 I, 123 I, 99 mTc, 225 Ac, 57 La, 67/69 Ga, 68 Ga, or 152 Eu.

13. The method of claim 1 , wherein the polymeric nanoparticle is conjugated or chelated to DOTA (1,4,7,10-tetraazacyclo-dodecane-1,4,7,10-tetraacetic acid), DTPA (diethylene triamine pentaacetic acid), DOTP (1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetra (methylene phosphonic) acid), DOTMA, (1R, 4R, 7R, 10R)-α′α″α′″-Tetramethyl-1,4,7,10-tetraazacyclododecane-1,4,7, 10-tetraacetic acid) tetrasodium salt, TETA, (1,4,8,11-Tetraazacyclotetradecane-1,4,8, 11-tetraacetic acid), DOTAM (1,4,7,10-Tetrakis (carbamoylmethyl)-1,4,7, 10-tetraazacyclododecane), CB-TE2A (1,4,8,11-tetraazabicyclo[6.6.2]hexadecane-4, 11-dicetic acid), and NOTA ((1,4,7-triazacyclononane-N,N′,N″-triacetic acid).

14. The method of claim 1 , wherein the polymeric nanoparticle further comprises a folate.

Assignments (1)

Continuity (3)

References Cited (200)