Conserved region T cell vaccines for coronavirus and methods of use
Immunogenic compositions and methods of their use in eliciting immune responses to coronaviruses, such as SARS-CoV-2 are provided.
1. An immunogenic composition comprising:
one or more polypeptides comprising the amino acid sequence of any one of SEQ ID NOs:5-7, or a nucleic acid encoding the one or more polypeptides; and
a pharmaceutically acceptable carrier.
2. The immunogenic composition of claim 1 , wherein the one or more polypeptides comprise:
SEQ ID NO: 6; or
SEQ ID NO: 5 and SEQ ID NO: 6; or
SEQ ID NO: 6 and SEQ ID NO: 7; or
SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7.
3. The immunogenic composition of claim 1 , wherein the nucleic acid is an mRNA and the mRNA is formulated in a lipid nanoparticle, and wherein the lipid nanoparticle comprises ALC-0315.
4. The immunogenic composition of claim 1 , wherein the one or more polypeptides comprise two or more polypeptides, and at least one of the two or more polypeptides further comprise SEQ ID NO: 1.
5. The immunogenic composition of claim 4 , wherein at least one of the two or more polypeptides comprise SEQ ID NO: 6.
6. The immunogenic composition of claim 4 , wherein the two or more polypeptides comprise:
SEQ ID NO: 1 and SEQ ID NO: 5; or
SEQ ID NO: 1 and SEQ ID NO: 6; or
SEQ ID NO: 1 and SEQ ID NO: 7; or
SEQ ID NO: 1, SEQ ID NO: 5, and SEQ ID NO: 6; or
SEQ ID NO: 1, SEQ ID NO: 5, and SEQ ID NO: 7; or
SEQ ID NO: 1, SEQ ID NO: 6, and SEQ ID NO: 7.
7. A vector, wherein the vector comprises a nucleic acid sequence encoding any one of SEQ ID NOs: 5-7.
8. The vector of claim 7 , wherein the vector is an adenovirus vector.
9. The vector of claim 8 , wherein the adenovirus vector is an Ad5, Ad26, Ad35, or Ad52 adenovirus vector or a ChAdO×1 or ChAdO×2 adenovirus vector.
10. A method of eliciting an immune response to a coronavirus in a subject, comprising administering to the subject the immunogenic composition of claim 1 ,
thereby eliciting an immune response to the coronavirus.
11. The method of claim 10 , wherein the one or more polypeptides of the immunogenic composition comprise:
SEQ ID NO: 6; or
SEQ ID NO: 5 and SEQ ID NO: 6; or
SEQ ID NO: 6 and SEQ ID NO: 7.
12. The method of claim 10 , wherein the immunogenic composition comprises the nucleic acid sequence encoding the one or more polypeptides, and the nucleic acid is mRNA.
13. The method of claim 12 , wherein the mRNA is formulated in a lipid nanoparticle, and wherein the lipid nanoparticle comprises ALC-0315.
14. The method of claim 10 , wherein the immunogenic composition comprises the nucleic acid sequence encoding the one or more polypeptides, and administering the nucleic acid comprises administering an adenovirus vector comprising the nucleic acid.
15. The method of claim 14 , wherein the adenovirus vector is an Ad5, Ad26, Ad35, or Ad52 adenovirus vector or a ChAdO×1 or ChAdO×2 adenovirus vector.