Compositions of asymmetric interfering RNA and uses thereof
The present invention provides asymmetrical duplex RNA molecules that are capable of effecting sequence-specific gene silencing. The RNA molecule comprises a first strand and a second strand. The first strand is longer than the second strand. The RNA molecule comprises a double-stranded region formed by the first strand and the second strand, and two ends independently selected from the group consisting of 5′-overhang, 3′-overhang, and blunt end. The RNA molecules of the present invention can be used as research tools and/or therapeutics.
1. An asymmetric interfering RNA duplex molecule for modulating expression of a target gene, comprising an antisense strand and a sense strand,
wherein the antisense strand is longer than the sense strand, has a length of 19, 20, 21, 22, 23, 24, 25, 26 or 27 nucleotides and includes a 3′-overhang of 1-9 nucleotides and a 5′-overhang of 0-8 nucleotides when duplexed with the sense strand;
wherein the sense strand has a length of 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 or 24 nucleotides and forms a double-stranded region with the antisense strand, and at least the first nucleotide and last nucleotide of the sense strand base pair with nucleotides of the antisense strand, and wherein the double-stranded region has a length of 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 or 24 base pair; and
wherein the antisense strand is at least 70% complementary to an expressed nucleotide sequence of the target gene and the last nucleotide of its 3′ end is complementary to the target sequence.
2. The RNA duplex molecule of claim 1 , wherein the sense strand of the RNA duplex does not mediate off-target silencing.
3. The RNA duplex molecule of claim 1 , wherein the antisense strand consists of 19, 20, 21, 22, 23, 24 or 25 nucleotides.
4. The RNA duplex molecule of claim 1 , wherein the antisense strand consists of 19, 20, 21, 22, 23, 24 or 25 nucleotides, and the sense strand consists of 12, 13, 14, 15, 16 or 17 nucleotides.
5. The RNA duplex molecule of claim 1 , wherein the antisense strand consists of 19, 20, 22, 23, 24 or 25 nucleotides, and the sense strand consists of 18, 19, 20, 21, 22, 23 or 24 nucleotides.
6. The RNA duplex molecule of claim 1 , wherein the antisense strand consists of 21 nucleotides, the sense strand consists of 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleotides, and wherein the antisense strand is not
(SEQ ID NO: 1)
5′-UUCGAAGUAUUCCGCGUACGU,
(SEQ ID NO: 2)
5′-UCGAAGUAUUCCGCGUACGUG
or
(SEQ ID NO: 3)
5′-CGAAGUAUUCCGCGUACGUGA.
7. The RNA duplex molecule of claim 1 , wherein the double-stranded region consists of perfectly complementary sequences.
8. The RNA duplex molecule of claim 1 , wherein the double-stranded region comprises at least one nick, gap or mismatch.
9. The RNA duplex molecule of claim 1 , wherein the double-stranded region comprises one mismatch base pair between the two strands.
10. The RNA duplex molecule of claim 1 , wherein the GC content of the double stranded region is 30%-50%.
11. The RNA duplex molecule of claim 1 , wherein the antisense strand comprises a 3′-overhang of 2, 3, 4, 5, 6 or 7 nucleotides.
12. The RNA duplex molecule of claim 1 , wherein the antisense strand comprises a 5′-overhang of 0, 1, 2, 3, 4 or 5 nucleotides.
13. The RNA duplex molecule of claim 1 , wherein the antisense strand comprises a 5′-blunt end.
14. The RNA duplex molecule of claim 1 , wherein at least one nucleotide of the 5′ end of the antisense strand is selected from the group consisting of A, U, and dT.
15. The RNA duplex molecule of claim 1 , wherein the 5′-end of the antisense oligonucleotide comprises an “AA”, “UU” or “dTdT” motif.
16. The RNA duplex molecule of claim 1 , wherein the 3′-overhang and/or 5′-overhang is stabilized against degradation either through chemical modification or secondary structure.
17. The RNA duplex molecule of claim 1 , wherein the last nucleotide at the 3′ end of the antisense strand consists of an A, U, G or C ribonucleotide.
18. The RNA duplex molecule of claim 1 , wherein the RNA duplex molecule contains at least one modified nucleotide or an analogue thereof.
19. The RNA duplex molecule of claim 18 , wherein the at least one modified nucleotide or an analogue thereof is selected from the group consisting of:
a) a sugar-, backbone-, and/or base-modified ribonucleotide;
b) a backbone-modified ribonucleotide with a modification in its phosphodiester linkage with another ribonucleotide;
c) a backbone-modified ribonucleotide with its phosphodiester linkage with another ribonucleotide modified to include at least one of a nitrogen or sulphur heteroatom;
d) a backbone-modified ribonucleotide containing a phosphothioate group;
e) a modified nucleotide or an analogue thereof having an unusual base or a modified base;
f) a modified nucleotide or an analogue thereof having an inosine or a tritylated base;
g) a sugar-modified ribonucleotide, wherein the 2′-OH group is replaced by a group selected from H, OR, R, halo, SH, SR, NH 2 , NHR, NR 2 and CN, wherein each R is independently C 1 -C 6 alkyl, alkenyl or alkynyl, and halo is F, Cl, Br or I; and
h) a sugar-modified ribonucleotide, wherein the 2′-OH is replaced by a 2′-O-methyl group and/or 2′-F.
20. The RNA duplex molecule of claim 1 , wherein the antisense strand comprises at least one deoxynucleotide.
21. The RNA molecule of claim 1 , wherein at least one nucleotide of the 5′-end of antisense strand is not complementary to the target gene sequence.
22. The RNA molecule of claim 1 , wherein the antisense strand and sense strand are joined by a chemical linker.
23. The RNA duplex molecule of claim 1 , wherein the sense strand and/or antisense strand is conjugated to a natural ligand.
24. The RNA duplex molecule of claim 1 being conjugated to an entity selected from the group consisting of peptide, antibody, polymer, lipid, oligonucleotide, cholesterol, and aptamer.
25. The RNA duplex molecule of claim 1 , wherein the target gene is selected from the group consisting of:
a) a gene associated with human or animal diseases,
b) a gene of a pathogenic microorganism,
c) a viral gene,
d) a tumor-associated gene, and
e) a gene associated with a disease selected from the group consisting of autoimmune diseases, inflammatory diseases, degenerative diseases, infectious diseases, proliferative diseases, metabolic diseases, immune-mediated disorders, allergic diseases, dermatological diseases, malignant diseases, gastrointestinal disorders, respiratory disorders, cardiovascular disorders, renal disorders, rheumatoid disorders, neurological disorders, endocrine disorders, and aging.
26. A composition comprising the RNA duplex molecule of claim 1 and a pharmaceutically acceptable excipient, carrier, or diluent.
27. The composition of claim 26 , wherein the pharmaceutically acceptable excipient, carrier, or diluent is selected from the group consisting of a pharmaceutical carrier, a positive-charge carrier, a liposome, a protein carrier, a polymer, a nanoparticle, a nanoemulsion, a lipid, and a lipoid.