Anti-PD-1 antibodies
The present disclosure provides monoclonal antibodies against protein programmed cell death 1 (PD-1), which can block the binding of PD-1 ligands to PD-1, and therefore block the inhibitory function of PD-1 ligands on PD-1 expressing T cells. The antibodies of disclosure provide very potent agents for the treatment of multiple cancers via modulating human immune function
1. An isolated polynucleotide encoding an antibody comprising a heavy chain variable region comprising SEQ ID NO: 53 and a light chain variable region comprising SEQ ID NO: 67.
2. The isolated polynucleotide of claim 1 , wherein the antibody is an IgG4 antibody.
3. An isolated polynucleotide encoding an scFv comprising a heavy chain variable region comprising SEQ ID NO: 53; and a light chain variable region comprising SEQ ID NO: 67.
4. A vector comprising the isolated polynucleotide of claim 1 .
5. A vector comprising the isolated polynucleotide of claim 3 .
6. A host cell comprising the vector of claim 4 .
7. A host cell comprising the vector of claim 5 .
8. A method for expressing an antibody comprising a heavy chain variable region comprising SEQ ID NO: 53; and a light chain variable region comprising SEQ ID NO: 67, the method comprising culturing the host cell of claim 5 under conditions at which an antibody comprising a heavy chain variable region comprising SEQ ID NO: 53; and a light chain variable region comprising SEQ ID NO: 67 is expressed.
9. The method of claim 8 , wherein the antibody is an IgG4 antibody.