Ultra-long acting insulin-Fc fusion protein and methods of use
The present disclosure relates to compositions of insulin-Fc fusion proteins and their use to treat diabetes.
1. A fusion protein comprising an insulin polypeptide and an Fc fragment, wherein the insulin polypeptide and the Fc fragment are connected by a peptide linker, wherein the fusion protein comprises the sequence:
(SEQ ID NO: 34)
FVNQHLCGSDLVEALALVCGERGFFYTDPTGGGPRRGIVEQCCHSICSLY
QLENYCNGGGGAGGGGDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMIS
RTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYKSTYRVVS
VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPS
RDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSF
FLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG.
2. The fusion protein of claim 1 , wherein the fusion protein is a homodimer comprising two identical monomers bound together via one or more disulfide bonds.
3. The fusion protein of claim 2 , wherein the percentage homodimer of insulin-Fc fusion protein is greater than or equal to 80%.